12 results on '"Ochs R"'
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2. Localization of nucleolar phosphoproteins B23 and C23 during mitosis
- Author
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Ochs, R., primary, Lischwe, M., additional, O'Leary, P., additional, and Busch, H., additional
- Published
- 1983
- Full Text
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3. Association of nuclear matrix proteins with granular and threaded nuclear bodies in cell lines undergoing apoptosis.
- Author
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Zweyer M, Riederer BM, Ochs RL, Fackelmayer FO, Kohwi-Shigematsu T, Bareggi R, Narducci P, and Martelli AM
- Subjects
- Antigens, Nuclear, Cell Line, Chromatin metabolism, Chromosomal Proteins, Non-Histone analysis, DNA, Endopeptidases metabolism, HL-60 Cells, Histones metabolism, Humans, Inclusion Bodies metabolism, Jurkat Cells, Promyelocytic Leukemia Protein, Transcription Factors analysis, Tumor Cells, Cultured, Tumor Suppressor Proteins, Apoptosis physiology, Biomarkers analysis, Neoplasm Proteins, Nuclear Proteins analysis
- Abstract
The granules which appear in the nucleolar area in apoptotic HL-60 cells after camptothecin administration (Zweyer et al., Exp. Cell Res. 221,27-40, 1995) were detected also in several other cell lines induced to undergo apoptosis by different stimuli, such as MOLT-4 treated with staurosporine, K-562 incubated with actinomycin D, P-815 exposed to temperature causing heat shock, Jurkat cells treated with EGTA, U-937 growing in the presence of cycloheximide and tumor necrosis factor-alpha, and HeLa cells treated with etoposide. Using immunoelectron microscopy techniques, we demonstrate that, besides the already described nuclear matrix proteins p125 and p160, these granules contain other nucleoskeletal polypeptides such as proliferating cell nuclear antigen, a component of ribonucleoprotein particles, a 105-kDa constituent of nuclear spliceosomes, and the 240-kDa nuclear mitotic apparatus-associated protein referred to as NuMA. Moreover, we also found in the granules SAF-A/hn-RNP-U and SATB1 proteins, two polypeptides that have been reported to bind scaffold-associated regions DNA sequences in vitro, thus mediating the formation of looped DNA structures in vivo. Fibrillarin and coilin are not present in these granules or the PML protein. Thus, the granules seen during the apoptotic process apparently are different from coiled bodies or other types of nuclear bodies. Furthermore, these granules do not contain chromatin components such as histones and DNA. Last, Western blotting analysis revealed that nuclear matrix proteins present in the granules are not proteolytically degraded except for the NuMA polypeptide. We propose that these granules might represent aggregates of nuclear matrix proteins forming during the apoptotic process. Moreover, since the granules are present in several cell lines undergoing apoptosis, they could be considered a previously unrecognized morphological hallmark of the apoptotic process.
- Published
- 1997
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4. Quantitative changes of the two major AgNOR proteins, nucleolin and protein B23, related to stimulation of rDNA transcription.
- Author
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Derenzini M, Sirri V, Pession A, Trerè D, Roussel P, Ochs RL, and Hernandez-Verdun D
- Subjects
- Animals, Blotting, Western, Hepatectomy, Molecular Weight, Nuclear Proteins analysis, Nucleophosmin, Phosphoproteins analysis, RNA, Ribosomal biosynthesis, Rats, Rats, Wistar, Reference Values, Nucleolin, DNA, Ribosomal metabolism, Liver metabolism, Liver Regeneration, Nuclear Proteins biosynthesis, Nucleolus Organizer Region metabolism, Phosphoproteins biosynthesis, RNA-Binding Proteins, Transcription, Genetic
- Abstract
The relationship between the amount of the two major AgNOR proteins, nucleolin and protein B23, and the rate of ribosomal RNA (rRNA) synthesis was studied in cortisol-treated and regenerating rat hepatocytes after partial hepatectomy. In both experimental models the synthesis of rRNA was greatly stimulated, but only in regenerating hepatocytes was the increased synthesis associated with cells entering the mitotic cycle. Nucleolin and protein B23 were identified on SDS-polyacrylamide gels of nuclear proteins transferred to nitrocellulose and detected (1) by immunoreaction with specific monoclonal antibodies revealed by second antibodies coupled to peroxidase followed by chemiluminescence or (2) by the silver-staining procedure for AgNOR proteins. Nucleolin and protein B23 were then quantified by computerized densitometric analysis of the immunolabeling signals or the silver-stained bands at 105 kDa (nucleolin) and 39 kDa (protein B23). The synthesis of rRNA was measured by evaluating the amount of radioactivity incorporated into pre-rRNA after [3H]orotic acid injection. Densitometric analysis of silver-stained bands and immunolabeling signals showed no change in nucleolin and protein B23 amounts in cortisol-stimulated hepatocytes, whereas a moderate increase was found in regenerating hepatocytes at 12 h after partial hepatectomy. In both cortisol-stimulated and regenerating hepatocytes the synthesis of rRNA was highly increased (2.6-fold and 4.3-fold above the control level, respectively). To ascertain the relationship between quantitative changes in nucleolin and protein B23 and stimulation of rRNA transcriptional activity in regenerating hepatocytes, the quantitative distribution of these proteins was also investigated in the early times of regeneration using silver-stained nitrocellulose-transblotted nuclear proteins. The quantity of protein B23 was unchanged until 12 h after partial hepatectomy, whereas nucleolin appeared to be slightly increased at 9 h (1.15-fold above the control value) after partial hepatectomy. On the other hand, at just 6 h after partial hepatectomy, a significant increase of rRNA synthesis occurred in regenerating rat hepatocytes (1.8-fold above the control value). These data demonstrated that stimulation of rRNA transcriptional activity occurring in rat hepatocytes after cortisol treatment and in the early times after partial hepatectomy was not associated with quantitative changes in the amounts of nucleolin and protein B23.
- Published
- 1995
- Full Text
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5. Nuclear bodies (NBs): a newly "rediscovered" organelle.
- Author
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Brasch K and Ochs RL
- Subjects
- Animals, Humans, Microscopy, Electron, Organelles metabolism, Cell Nucleus ultrastructure, Organelles ultrastructure
- Abstract
Nuclear bodies (NBs) were first described in detail some 30 years ago, by conventional electron microscopy, as prominent interchromatin structures found primarily in the nuclei of malignant or hyperstimulated animal cells. Subsequent studies have shown that NBs are ubiquitous organelles, but they are numerically and morphologically quite varied. With the recent discovery of human autoantibodies against several key nuclear antigens present in some NBs, these structures are once again the subject of much attention. At least one class of NBs, coiled bodies, has been shown to be nucleolus-derived and to contain not only nucleolus-associated antigens, but also many of the snRNP components involved in pre-mRNA splicing. These data suggest that coiled bodies, and perhaps other NBs as well, are multifunctional and may be involved in the processing or transport of both pre-mRNA and pre-rRNA. Further evidence is provided showing that NBs constitute distinct nuclear domains whose functional significance is just now emerging.
- Published
- 1992
- Full Text
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6. Centromere autoantigens are associated with the nucleolus.
- Author
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Ochs RL and Press RI
- Subjects
- Autoantigens chemistry, Dactinomycin pharmacology, Fluorescent Antibody Technique, HeLa Cells, Humans, Interphase, Microscopy, Electron, Molecular Weight, Scleroderma, Systemic immunology, Autoantigens metabolism, Cell Nucleolus ultrastructure, Centromere ultrastructure
- Abstract
Because of their importance as target antigens in scleroderma and since all other major autoantigens in scleroderma can be localized to the interphase nucleolus, we were interested in a further investigation of the potential relationship between interphase centromeres and the nucleolus. Using human anticentromere autoantibodies (ACA) from patients with the CREST form of scleroderma as probes in indirect immunofluorescence microscopy, we observed nonrandom interphase "clumping" of centromeres in a distribution suggestive of nucleoli. By double-label immunofluorescence comparing the localization of centromeres to nucleolar proteins Ki-67, fibrillarin, or protein B23 (nucleophosmin), interphase centromeres appeared to be localized around and within nucleoli. A number of different ACA sera were tested on HEp-2, HeLa, PtK2, Indian muntjac, 3T3, and NRK cells, all with identical results indicating colocalization between centromeres and nucleoli. Immunoelectron microscopy revealed that interphase centromeres were distributed free in the nucleoplasm, in contact with the nuclear envelope, in contact with and on the periphery of nucleoli, and totally embedded within the confines of the nucleolus itself. Interestingly, actinomycin D treatment dissociated centromeres from localization within the segregated nucleolus. To determine if interphase centromeres were integral components of nucleoli, nucleoli were isolated according to classical methods. By double-label immunofluorescence, immunoelectron microscopy, and Western blotting, it was demonstrated that centromere autoantigens copurified with isolated nucleoli. These studies offer proof that some interphase centromeres can be associated with, and may even be considered part of, the interphase nucleolus. Furthermore, all of the major autoantigens in scleroderma can now be localized to the nucleolus.
- Published
- 1992
- Full Text
- View/download PDF
7. Detection of fibrillarin in nucleolar remnants and the nucleolar matrix.
- Author
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Ochs RL and Smetana K
- Subjects
- Animals, Cells, Cultured, Chickens, Erythrocytes ultrastructure, Female, Fluorescent Antibody Technique, HeLa Cells, Humans, Liver ultrastructure, Microscopy, Electron methods, Nuclear Matrix ultrastructure, Oocytes ultrastructure, Ribonucleoproteins analysis, Xenopus laevis, Cell Nucleolus ultrastructure, Chromosomal Proteins, Non-Histone analysis, Nucleolus Organizer Region ultrastructure
- Abstract
In order to gain further insights into the fundamental structure of the nucleolus, nucleolar remnants of Xenopus and chickens were examined for the presence of fibrillarin and nucleolus organizer region (NOR) silver staining. Nucleolar remnants of Xenopus nucleated red blood cells were found to contain easily detectable amounts of fibrillarin and NOR silver staining. Upon examination of various tissues, fibrillarin and NOR silver staining were detected in nucleoli of Xenopus liver hepatocytes and within nucleoli of oocytes and follicle cells from ovaries of mature female toads. By comparison, nucleolar remnants of adult chicken nucleated red blood cells contained only trace amounts of fibrillarin and NOR silver staining, whereas red blood cell nucleolar remnants of immature chicks had easily detectable amounts of fibrillarin and NOR silver staining. Nucleoli from hepatocytes of both adult and immature chickens demonstrated comparable levels of fibrillarin and NOR silver staining. Since fibrillarin was found in nucleolar remnant structures, we tested for (and detected) its presence in residual nucleoli of in situ nuclear matrix derived from HeLa cells. These findings are discussed in terms of the basic structural and functional organization of the nucleolus.
- Published
- 1991
- Full Text
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8. Immunological and ultrastructural studies of the nuclear coiled body with autoimmune antibodies.
- Author
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Raska I, Andrade LE, Ochs RL, Chan EK, Chang CM, Roos G, and Tan EM
- Subjects
- Animals, Blotting, Western, Cell Line, Chromosomal Proteins, Non-Histone metabolism, Humans, Mice, Microscopy, Electron, Nuclear Proteins metabolism, Rats, Autoantibodies immunology, Autoantigens immunology, Cell Nucleus ultrastructure, Nuclear Proteins immunology
- Abstract
Studies with human autoimmune sera identified auto-antibodies reacting with a novel antigen of 80 kDa. In interphase mammalian cells, the 80-kDa antigen was enriched in nuclear coiled bodies and was used as a marker for this nuclear structure. This antigen was subsequently named p80-coilin. By light and electron microscopic immunocytochemistry, a number of other antigens were also localized to the coiled body, including components of small nuclear ribonucleoproteins which are involved in the processing of nucleolar and extranucleolar RNA. Although the function of the coiled body is unknown, the presence of these subcellular particles might indicate an involvement in RNA metabolism. The identification of a protein highly enriched in this structure and the availability of specific antibodies might help in its isolation and the study of its function.
- Published
- 1991
- Full Text
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9. Further evidence that phosphoprotein C23 (110 kD/pI 5.1) is the nucleolar silver staining protein.
- Author
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Ochs RL and Busch H
- Subjects
- Animals, Antibodies, Monoclonal, Cell Line, Interphase, Macropodidae, Mitosis, Phosphoproteins immunology, Silver, Staining and Labeling, Cell Nucleolus analysis, Nucleolus Organizer Region analysis, Phosphoproteins analysis
- Abstract
Previously we demonstrated a similar distribution between nucleolar organizing region-(NOR)-specific silver staining and localization of nucleolar phosphoprotein C23 (MW 110 kD/pI 5.1) [1, 2]. We now report that under fixation conditions which allow for antibody binding and subsequent silver staining, monoclonal antibody against protein C23 blocks NOR silver staining as well as silver staining in interphase nucleoli. Monoclonal antibody against nucleolar phosphoprotein B23 (MW 37 kD/pI 5.1) did not block silver staining in either NORs or interphase nucleoli. These, along with earlier observations, provide evidence that nucleolar phosphoprotein C23 is the major silver staining protein of the nucleolus and that it is directly or indirectly associated with rDNA.
- Published
- 1984
- Full Text
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10. Immunofluorescence studies on proteins B23 and C23 in nucleoli of human lymphocytes.
- Author
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Smetana K, Ochs R, Lischwe MA, Gyorkey F, Freireich E, Chudomel V, and Busch H
- Subjects
- Cell Nucleolus ultrastructure, Fluorescent Antibody Technique, Humans, Leukemia, Lymphoid drug therapy, Lymphocytes ultrastructure, Nucleophosmin, RNA blood, Cell Nucleolus analysis, Leukemia, Lymphoid blood, Lymphocytes analysis, Nuclear Proteins, Phosphoproteins blood, Ribonucleoproteins blood
- Abstract
Nucleoli of normal and leukemic lymphocytes were studied by cytochemical and immunofluorescence methods to provide more information on the nucleolar presence and distribution of proteins B23 and C23. Annular nucleoli of human lymphocytes represent a very convenient subject for such studies, since they consist of one centrally located large fibrillar center surrounded by RNP components. In such nucleoli, protein C23 was present mainly in the central nucleolar region and protein B23 was found mostly in the periphery. The nucleolar area immunostained for protein B23 was usually larger than that stained for protein C23. The distribution of protein C23 appeared to be similar to that of intensely stained nucleolar argyrophilic components. No substantial differences were found between the distribution of proteins B23 and C23 in nucleoli of normal and leukemic lymphocytes. In lymphocytes of patients treated with chemotherapy, the immunofluorescence was diminished for protein B23 and particularly so for protein C23.
- Published
- 1984
- Full Text
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11. Intermediate filament proteins in human sperm heads.
- Author
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Ochs D, Wolf DP, and Ochs RL
- Subjects
- Antibodies, Monoclonal, Electrophoresis, Polyacrylamide Gel, Humans, Immunologic Techniques, Intermediate Filament Proteins immunology, Male, Microscopy, Fluorescence, Intermediate Filament Proteins metabolism, Sperm Head metabolism, Spermatozoa metabolism
- Abstract
Monoclonal antibodies made against human sperm cells have been characterized with regard to binding patterns and molecular coordinates of the recognized antigens. Antibodies T5 and T6 gave uniform binding to the acrosomal cap in an intact cell, and decreased to equatorial segment binding in an 'acrosome-reacted' cell. Monoclonal antibody T15 gave the reverse: equatorial segment binding in intact cells and uniform acrosomal cap binding in reacted cells. From staining patterns on cultured cell lines, determination of molecular coordinates, immunoblots, and partial peptide analysis, we have determined that T15 is directed against the cytoskeletal protein, vimentin, while T5 and T6 recognize a keratin-like protein which may be unique to sperm cells. This is the first immunological and biochemical study to analyse both types of intermediate filament proteins in human sperm cells.
- Published
- 1986
- Full Text
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12. Fibrillar center distribution in nucleoli of PHA-stimulated human lymphocytes.
- Author
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Ochs RL and Smetana K
- Subjects
- Cell Cycle, Cell Nucleolus analysis, Fluorescent Antibody Technique, Histological Techniques, Humans, Immunohistochemistry, Microscopy, Electron, RNA analysis, RNA Polymerase I metabolism, Staining and Labeling, Cell Nucleolus ultrastructure, Lymphocyte Activation
- Abstract
We have utilized acidic toluidine blue staining for RNA and immunofluorescence staining for RNA polymerase 1 to visualize the distribution of fibrillar centers (FCs) in nucleoli of PHA-stimulated human lymphocytes. At 0 h, there is a single large fibrillar center in each nucleolus which splits into smaller and more numerous FCs until the number of FCs reaches five, the number of nucleolus organizers in normal haploid human cells. With time, each FC then "unwinds" to form linear arrays of smaller FCs until the maximum number of FCs approaches the ribosomal gene copy number of 200 at 48 h in culture. It is hypothesized that in the most active state, each nucleolar FC visualized by RNA polymerase 1 staining actually represents a single transcription unit and the distance between adjacent FCs is occupied by the nontranscribed spacer region. We conclude that the number of fibrillar centers per nucleolus can be used as a direct quantitative measure of nucleolar transcriptional activity.
- Published
- 1989
- Full Text
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