1. Eine methode zur darstellung der gestalt von chromosomen-oberflächen im lichtmikroskop
- Author
-
I.H. Pawlowitzki and G. Pfefferkorn
- Subjects
Surface (mathematics) ,genetic structures ,business.industry ,G banding ,Thin layer ,Chromosome ,Cell Biology ,Biology ,Surface shape ,Giemsa stain ,law.invention ,Optics ,Optical microscope ,law ,Microscopy ,business - Abstract
Visualization of chromosomal surface architecture using a new light-microscopical method A new method is described, by which the surface architecture of chromosomes can be visualized using light microscope. The principles of the method include 1. 1. the coating of chromosomes with a thin layer of evaporated gold to increase light reflection; 2. 2. the use of oblique and asymmetrical illumination which creates shadows across the surface of chromosomes such that elevations appear bright and depression dark. The chromosomes thus give a three-dimensional appearance. This method can be used to demonstrate correlations between the Giemsa banding pattern and the surface architecture of chromosomes: each Giemsa positive area clearly corresponds to an elevation of the chromosomal surface.
- Published
- 1973