1. Production and rearing of germ-free X-SCID pigs
- Author
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Yutaka Hanazono, Hiroaki Shibata, Hideki Uosaki, Osamu Nureki, Hiroshi Nagashima, Satoshi Kunita, Shuji Hishikawa, Kazuaki Nakano, Tomoyuki Abe, Masahito Watanabe, Hiromasa Hara, and Takahiro Ohnuki
- Subjects
0301 basic medicine ,Litter (animal) ,Resuscitation ,Microbiological culture ,germ-free pig ,Original ,Swine ,medicine.medical_treatment ,Biology ,Hysterectomy ,Infections ,X-Linked Combined Immunodeficiency Diseases ,General Biochemistry, Genetics and Molecular Biology ,Uterus excision ,Andrology ,03 medical and health sciences ,Pregnancy ,medicine ,Animals ,Derivation ,gnotobiote ,Animal Husbandry ,Severe combined immunodeficiency ,General Veterinary ,General Medicine ,medicine.disease ,Specific Pathogen-Free Organisms ,Disease Models, Animal ,030104 developmental biology ,interleukin-2 receptor gamma chain gene (IL2RG) knock-out ,Mutation ,Animal Science and Zoology ,Female ,Disease Susceptibility ,X-linked severe combined immunodeficiency (X-SCID) ,Interleukin Receptor Common gamma Subunit - Abstract
Pigs with X-linked severe combined immunodeficiency (X-SCID) caused by a mutation of the interleukin-2 receptor gamma chain gene (IL2RG) are of value for a wide range of studies. However, they do not survive longer than 8 weeks because of their susceptibility to infections. To allow longer survival of X-SCID pigs, the animals must be born and reared under germ-free conditions. Here, we established an efficient system for piglet derivation by hysterectomy and used it to obtain and maintain a germ-free X-SCID pig. In four trials using pregnant wild-type pigs, 66% of piglets after hysterectomy started spontaneous breathing (range of 20-100% per litter). The resuscitation rate was found to negatively correlate with elapsed time from the uterus excision to piglet derivation (r=-0.97, P
- Published
- 2017