1. PCA3 gene expression in prostate cancer tissue in a Chinese population: Quantification by real-time FQ-RT-PCR based on exon 3 of PCA3
- Author
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Cheng-di Li, Xiao-hua Zhang, Zhi-liang Weng, Xiao-lu Mao, Kaiyuan Yu, Zhihua Tao, Mo Shen, Ou-chen Wang, Yan-Bo Zheng, Qi-tong Song, Zhixian Yu, Hui Xie, Yibing Yin, Yuan-Ping Hu, Xiuling Wu, and Zhan-guo Chen
- Subjects
Male ,PCA3 ,Pathology ,medicine.medical_specialty ,Clinical Biochemistry ,Prostatic Hyperplasia ,Pathology and Forensic Medicine ,Prostate cancer ,Exon ,Asian People ,Antigens, Neoplasm ,Biomarkers, Tumor ,Humans ,Medicine ,RNA, Messenger ,Molecular Biology ,Neoplasm Staging ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,Prostatic Neoplasms ,Cancer ,Exons ,Amplicon ,Prognosis ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Real-time polymerase chain reaction ,Cancer research ,Biomarker (medicine) ,Primer (molecular biology) ,business - Abstract
Prostate cancer (PCa) is the second most common cancer in men, and its incidence is still increasing. PCA3 is the most prostate cancer specific biomarker. Here we confirmed that both exon 3 and exon 4 are in the prostate-specific region of the PCA3 gene, and established the methodology of real-time fluorescent quantitative RT-PCR (FQ-RT-PCR) detecting PCA3 mRNA with primer spanning exons 1 and 3, and evaluated its clinical utility in a Chinese population. What disclosed that PCA3 mRNA is prostate cancer specific and shows increased expression in prostate cancer. It could be a reliable molecular marker in prostate cancer diagnosis. Exon 3-based real-time FQ-RT-PCR may prove useful in prostate cancer diagnosis, given that the associated primer would span only exons 1 and 3, relative to other models spanning exons 1 to 4. A shorter amplicon would not only enhance the efficiency of real-time FQ-RT-PCR, but may also simplify the quantification of PCA3 mRNA.
- Published
- 2010
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