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The effect of heat shock on protein synthesis in three related Drosophila species belonging to the obscura group was analyzed on SDS-acrylamide gels. Four major heat shock proteins (hsps) were found in these species, in which synthesis reaches a maximum at 34°C. Although the higher molecular weight proteins are conserved, differences in size were found for the small hsps in these species. By means of in situ hybridization using D. melanogaster probes for the small hsp genes, it was inferred that the small hsp genes of the obscura group species are clustered at the 27A locus in all three species. [ABSTRACT FROM AUTHOR]

When individuals of Drosophila guanche are submitted to heat shock, five new puffs are induced. These puffs usually do not appear during normal development. Comparing these results with those obtained in Drosophila subobscura, also belonging to the obscura group, differences between the induced puffing pattern of both species have been found. [ABSTRACT FROM AUTHOR]

The karyotype of Microtus cabrerae, a species endemic to Spain, is described. It comprises 2n=54 chromosomes, with a 'fundamental number' of 64, and with very large sex chromosomes. An evolutionary relationship between this species and M. chrotorrhinus is suggested. [ABSTRACT FROM AUTHOR]