1. Immunomodulatory Effects of Hydrolyzed Seawater Pearl Tablet (HSPT) on Th1/Th2 Functionality in a Mice Model of Chronic Obstructive Pulmonary Disease (COPD) Induced by Cigarette Smoke
- Author
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Tai-Jin Lan, Zhongmin Zhang, Qiang-Qiang Yan, Jiang Lin, Zhenxing Chen, Si-Yin Han, Peng Liu, and Yong Lin
- Subjects
CD3 ,Pulmonary disease ,Spleen ,Inflammation ,Review Article ,Pharmacology ,03 medical and health sciences ,Other systems of medicine ,0302 clinical medicine ,medicine ,030304 developmental biology ,0303 health sciences ,COPD ,Lung ,medicine.diagnostic_test ,biology ,business.industry ,medicine.disease ,respiratory tract diseases ,Bronchoalveolar lavage ,medicine.anatomical_structure ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,biology.protein ,medicine.symptom ,business ,Infiltration (medical) ,RZ201-999 - Abstract
Chronic obstructive pulmonary disease (COPD) is predicted to become the third leading cause of death around the world. The present study is designed to investigate whether hydrolyzed seawater pearl tablet (HSPT) has immunoregulatory effects on the Th1/Th2 functionality in cigarette smoke-induced COPD model mice. The determination of the amino acid composition of HSPT was carried out by high-performance liquid chromatography (HPLC) with precolumn phenylisothiocyanate (PITC) derivatization. COPD model mice were constructed by cigarette smoking (CS) treatment and HSPT was administered. HSPT inhibited the infiltration of inflammation in the airway of the lung, reduced influx of eosinophils (EOSs), lymphocytes (LYMs), neutrophils (NEUs), and macrophages (MACs) in the bronchoalveolar lavage fluid (BALF), decreased the levels of IFN-γ, IL-2, IL-4, and IL-10 in the serum and lung, and decreased the expression of aforementioned cytokines in the spleen and lung in CS-treated mice. Besides, HSPT also had the ability to reduce the amount of CD3+CD4+ T cells and modulate the Th1/Th2 balance. Taken together, this study supports the consensus that CS is a critical factor to induce and aggravate COPD. HSPT could regulate the balance of Th1/Th2 in CS-induced COPD model mice, indicating its effects on inhibiting the development of COPD.
- Published
- 2020