1. Metabolomics Profiling to Investigate the Pharmacologic Mechanisms of Berberine for the Treatment of High-Fat Diet-Induced Nonalcoholic Steatohepatitis.
- Author
-
Jian Li, Zezhou Liu, Mingxing Guo, Kejia Xu, Miao Jiang, Aiping Lu, and Xiaoyan Gao
- Subjects
- *
LIPID metabolism , *ALKALOIDS , *ANIMAL experimentation , *BIOMARKERS , *BIOLOGICAL assay , *CYTOKINES , *DIET , *FATTY liver , *FAT content of food , *HIGH performance liquid chromatography , *INFLAMMATION , *MASS spectrometry , *PHOSPHOLIPIDS , *RATS , *RESEARCH funding , *STATISTICAL sampling , *T-test (Statistics) , *UNSATURATED fatty acids , *DATA analysis software , *DESCRIPTIVE statistics , *MANN Whitney U Test , *ONE-way analysis of variance - Abstract
Objective. Berberine has been used to treat nonalcoholic steatohepatitis (NASH), which has been addressed in many studies. In this study, we investigated the molecular pharmacology mechanisms of berberine using metabolomic techniques. Methods. Sprague-Dawley rats were randomly divided into three groups (10 rats in each group): (i) normal control group; (ii) high-fat diet- (HFD-) induced NASH model group; and (iii) HFD berberine-treated group (i.d. 200mg/kg). The handling procedure lasted eight weeks. Then, UPLC-Q-TOF/MS techniques coupled with histopathology and biochemical analyses were adopted to explore the mechanisms of berberine on the protective effects against NASH. Key Findings. (i) According to conventional test results, berberine treatment plays a fighting role in HFD-induced NASH due to its beneficial effects against insulin resistance, inflammation, and lipid metabolism. (ii) Based on UPLC-Q-TOF/MS techniques, metabolic profiles that involved sphingomyelin (SM), phosphatidylcholine (PC), lysophosphatidylcholine (LysoPC), 13-hydroperoxy-9, 11-octadecadienoic acid (13-HpODE), eicosatrienoic acid, docosatrienoic acid, and eicosenoic acid could provide potential metabolic biomarkers to address the pharmacological mechanisms of berberine. Conclusions. The parts of molecular pharmacological mechanisms of berberine for NASH treatment are related to the regulation of metabolic disruption involving phospholipid and unsaturated fatty acids in rats with NASH. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF