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Growing evidence has revealed that circular RNAs (circRNAs) play important roles in the development of cancers, including colorectal cancer (CRC). In this study, we mainly focused on the expression of circ_0056618 and potential functions of circ_0056618 in CRC patients.RT-PCR was performed to detect circ_0056618 and miR-206 expressions in CRC tissues and adjacent non-tumor tissues. Correlation analysis was used to analyze the correlation between circ_0056618 and miR-206. Kaplan-Meier method was conducted to analyze the overall survival (OS) for CRC patients. Moreover, CCK-8 assay was used to measure cell proliferation ability and transwell assay was performed to detect cell migration ability. Besides, tube formation assay was performed to measure cell angiogenesis capacity. Western blot (WB) was performed to measure protein levels of tissues samples and CRC cell lines. Notably, the Luciferase reporter assay was performed to prove the binding sites in circ_0056618 with miR-206, miR-206 with CXCR4 and VEGF-A.We found that circ_0056618 was elevated in CRC tumor tissues and CRC cell lines, which was related to poor diagnosis for CRC patients. MiR-206 was reduced in CRC tissues, which was negatively related with circ_0056618. Protein levels of CXCR4 and VEGF-A were elevated in CRC tumor tissues, which were negatively related with miR-206. Circ_0056618 inhibition inhibited proliferation, angiogenesis and migration of HT29 cells, and repressed protein levels of Cyclin D1, VEGF-A and N-cadherin and increased E-cadherin. Notably, Luciferase reporter assay indicated that circ_0056618 could sponge with miR-206, which could directly target at CXCR4 and VEGF-A. Finally, we proved a pathway that circ_0056618 promoted cell proliferation, migration and angiogenesis through sponging with miR-206 and removing the repressing effects of miR-206, thereby upregulating CXCR4 and VEGF-A in CRC.Above all, this study revealed that circ_0056618 was increased in CRC tissues, which was related with the poor OS of CRC patients. We found that circ_0056618 could promote cell proliferation, migration and angiogenesis through sponging with miR-206 and upregulating CXCR4 and VEGF-A in CRC, which might provide a novel potential therapeutic target for treating CRC.

The aim is to determine the efficacy and specific mechanism of microRNA-543-5p (miR-543-5p) on spinal cord injury (SCI).The model of spinal cord injury was established in 6-week-old rats. Firstly, quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the changes of miR-543-5p in the spinal cord of rats in each group after spinal cord injury. Next, we observed the alterations in nuclear factor-kappa B (NF-κB) RNA level after injection of miR-543-5p. In addition, the enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of the inflammatory cytokines, and we used Western blotting to detect the protein associated with nerve regeneration at the protein levels. Finally, the Basso-Beattie-Bresnahan (BBB) rating scale was utilized to measure the recovery of hindlimbs function in rats.After spinal cord injury, the RNA expression of miR-543-5p in the rat spinal cord was decreased, and the RNA level of NF-κB was found to be decreased after the artificial injection of miR-543-5p. In the inflammatory expression, we found that the expression of various inflammatory mediators was also downregulated. However, the expression of nerve regeneration related factors was significantly upregulated, and it was observed that the evaluated score of the miR-543-5p group was higher than that of the SCI group within 1 month.MiR-543-5p inhibited NF-κB pathway and reduced the inflammatory factors, and ameliorated nerve regeneration, which ultimately promoted hindlimbs locomotor function.

The aim of this study was to investigate clinical application of remifentanil in local anesthesia for resection of tumors in functional brain area.Twenty-four patients were randomly divided into two groups: control group and remifentanil group. In remifentanil group remifentanil was infused intravenously with micro pump in 0.05-0.1 µg·kg-1·min-1. The hemodynamic changes and complications during operation were monitored.The satisfactory degree for surgical procedure was evaluated. The surgery of two groups could be completed in a conscious state. Mean artery pressure (MAP), heart rate (HR) in remifentanil group during opening or closing skull or intra- cranial period were significantly lower than control group (p0.05). There were no conspicuous complications in two groups such as respiratory depression, nausea, vomitting and dysphoria. Satisfaction rate of remifentanyl group was significantly higher than control group (p0.05).Awake brain tumor surgery could be completed in rational use of remifentanil on the base of good local anesthesia, and hemodynamics were stable and the patients were well tolerated.