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Your search keyword '"D, Spallarossa"' showing total 3 results
Start Over Author "D, Spallarossa" Journal european respiratory journal
3 results on '"D, Spallarossa"'

1. Orally exhaled nitric oxide levels are related to the degree of blood eosinophilia in atopic children with mild-intermittent asthma

Author

Michela Silvestri, B. Fregonese, Elena Battistini, D. Spallarossa, G. A. Rossi, and V Frangova Yourukova

Subjects
Hypersensitivity, Immediate, Male, Pulmonary and Respiratory Medicine, Allergy, medicine.medical_specialty, Adolescent, Vital Capacity, Maximal Midexpiratory Flow Rate, Nitric Oxide, Gastroenterology, Nitric oxide, Atopy, Leukocyte Count, chemistry.chemical_compound, Forced Expiratory Volume, Internal medicine, medicine, Humans, Eosinophilia, Child, Skin Tests, Asthma, business.industry, Respiratory disease, medicine.disease, respiratory tract diseases, Eosinophils, Breath Tests, chemistry, Child, Preschool, Luminescent Measurements, Exhaled nitric oxide, Immunology, Female, medicine.symptom, business, Airway
Abstract

Increased levels of nitric oxide have been found in expired air of patients with asthma, and these are thought to be related to the airway inflammatory events that characterize this disorder. Since, in adults, bronchial inflammatory changes are present even in mild disease, the present study was designed to evaluate whether a significant proportion of children with mild-intermittent asthma could have increased exhaled air NO concentrations. Twenty-two atopic children (aged 11.1+/-0.8 yrs) with mild-intermittent asthma, treated only with inhaled beta2-adrenoreceptor agonists on demand and 22 age-matched controls were studied. NO concentrations in orally exhaled air, measured by chemiluminescence, were significantly higher in asthmatics, as compared to controls (19.4+/-3.3 parts per billion (ppb) and 4.0+/-0.5 ppb, respectively; p0.01). Interestingly, 14 out of 22 asthmatic children had NO levels8.8 ppb (i.e.2 standard deviations of the mean in controls). In asthmatic patients, but not in control subjects, statistically significant correlations were found between exhaled NO levels and absolute number or percentage of blood eosinophils (r=0.63 and 0.56, respectively; p0.01, each comparison). In contrast, exhaled NO levels were not correlated with forced expiratory volume in one second (FEV1) or forced expiratory flows at 25-75% of vital capacity (FEF25-75%) or forced vital capacity (FVC), either in control subjects, or in asthmatic patients (p0.1, each correlation). These results suggest that a significant proportion of children with mild-intermittent asthma may have airway inflammation, as shown by the presence of elevated levels of nitric oxide in the exhaled air. The clinical relevance of this observation remains to be established.

Published
1999
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2. Stimulation of eosinophil IgE low-affinity receptor leads to increased adhesion molecule expression and cell migration.

Author

Lantero S, Alessandri G, Spallarossa D, Scarso L, and Rossi GA

Subjects
Adolescent, Antibodies, Monoclonal pharmacology, Asthma blood, Asthma etiology, Binding, Competitive, Cell Adhesion Molecules immunology, Cell Adhesion Molecules physiology, Cell Movement drug effects, Cell Movement physiology, Child, Endothelium, Vascular cytology, Female, Humans, Hypersensitivity complications, Integrins metabolism, Ligands, Male, Receptors, IgE drug effects, Receptors, IgE immunology, Reference Values, Umbilical Cord blood supply, Cell Adhesion Molecules metabolism, Eosinophils metabolism, Receptors, IgE metabolism
Abstract

Immunoglobulin binding on eosinophil surface receptors results in activation of these cells. Evaluating blood eosinophils from atopic subjects, it was investigated whether ligation of immunoglobulin E low-affinity receptor (FcepsilonRII/ CD23) with specific monoclonal antibodies (Mabs) resulted in enhanced eosinophil migration and adhesion molecule expression. Eosinophils from 20 subjects with allergic asthma (atopic individuals) and nine nonatopic normal individuals (controls) were purified using Percoll gradients. The effect of antihuman CD23 Mabs on: 1) eosinophil migration through human umbilical vein endothelial cells (HUVECs); and 2) eosinophil expression of the adhesion molecules leukocyte function-associated antigen-1 (LFA-1, CD11a/CD18), macrophage antigen-1 (Mac-1, CD11b/CD18) and very late activation antigen-1 (VLA-4, CD49d/CD29) was evaluated by specific Mab staining and flow cytometric analysis. As compared to controls, freshly isolated eosinophils from atopic individuals showed enhanced migration through HUVECs (p<0.05) and increased LFA-1 expression (p<0.01), but similar Mac-1 and VLA-4 expression (p>0.1 for both). In both controls and atopic individuals, eosinophil incubation with antihuman CD23 Mabs induced a dose-dependent increase in cell migration through HUVECs, significant at antihuman CD23 Mab concentrations of 5 microg x mL(-1) (p>0.05 for all). Similarly, incubation of the cells with antihuman CD23 Mabs induced dose-dependent upregulation of LFA-1 and Mac-1 expression, whereas no changes in VLA-4 expression were observed (p>0.1). Finally, the enhanced eosinophil migration induced by antihuman CD23 Mab stimulation was significantly inhibited by antihuman LFA-1 (84+/-14% (mean+/-SEM); p<0.01) and VLA-4 Mabs (47+/-15%; p<0.05) but not by antihuman Mac-1 Mabs (p>0.1). In both atopic and control subjects, immunoglobulin E, low-affinity receptor stimulation induces functional changes in eosinophils characterized by increased eosinophil migration associated with enhanced late function antigen-1 and Mac-1 expression.

Published
2000
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3. LFA-1 expression by blood eosinophils is increased in atopic asthmatic children and is involved in eosinophil locomotion.

Author

Lantero S, Alessandri G, Spallarossa D, Scarso L, and Rossi GA

Subjects
Asthma complications, Asthma physiopathology, Cell Adhesion Molecules blood, Child, Child, Preschool, Endothelium, Vascular cytology, Female, Humans, Hypersensitivity, Immediate complications, In Vitro Techniques, Integrin alpha4beta1, Integrins blood, Leukocyte Count, Macrophage-1 Antigen blood, Male, Receptors, Lymphocyte Homing blood, Receptors, Very Late Antigen blood, Asthma immunology, Cell Movement physiology, Eosinophils immunology, Eosinophils physiology, Hypersensitivity, Immediate immunology, Lymphocyte Function-Associated Antigen-1 blood
Abstract

Allergic asthma is characterized by eosinophil migration in the airways, which is strictly dependent on the expression of adhesion molecules. This study investigated whether the expression of adhesion molecules on eosinophils is increased and associated with disease activity in allergic asthma. Twenty atopic asthmatic (AA) subjects and nine controls were studied and the expression of lymphocyte function-associated antigen-1 (LFA-1; CD11a/CD18), Mac-1 (CD11b/CD18) and very late antigen-4 (VLA-4; CD49d/CD29) on blood eosinophils was evaluated by specific monoclonal antibody (Mab) staining and flow-cytometric analysis. Compared with controls, eosinophils from AA showed increased expression of LFA-1 (p<0.005), but not of Mac-1 or VLA-4 (p>0.1). In addition, LFA-1 expression correlated positively with blood eosinophil number (r=0.792, p<0.05), while no correlations were observed between Mac-1 or VLA-4 expression and blood eosinophil number. The migration of eosinophils through human umbilical vein endothelial cells with or without anti-LFA-1, Mac-1 and VLA-4-blocking Mab was studied. Compared with controls, eosinophils from AA showed increased migration toward C5a (p<0.01). Cell migration was totally inhibited by preincubating eosinophils with anti-LFA-1 (p<0.05), while anti-Mac-1 had no effect (p>0.1). Thus, the expression of lymphocyte function-associated antigen-1 by blood eosinophils is increased in atopic asthmatics and seems to modulate the enhanced eosinophil migration observed in allergic asthma.

Published
1998
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