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The objective of the work was to study the qualitative composition and determine the quantitative content of phenolic compounds of Daucus carota subsp. sativus fruits of Yaskrava, Nantska Kharkivska and Olenka species and of Dauci carotae subsp. sativi fructuum extractum siccum. Phenolic compounds were studied by UPLC-ESI-MS/MS method. High content of phenol-origin bioactive substances was fixed in Daucus carota subsp. sativus fruits of Nantska Kharkivska variety (363.19 μg/g). Dominating compounds were chlorogenic acid, cynaroside, rutin and hyperoside. Daucus carota subsp. sativus fruits of all study species under question were found to possess permanent qualitative composition of phenolic compounds, whereas their quantitative content in experimental samples differed slightly. The results of analysis confirm promising development of novel antioxidant and cardioprotective drugs on the basis of Dauci carotae subsp. sativi fructuum extractum siccum.