This study aimed to address the effects of increased longitudinal bending stiffness (LBS) on running economy (RE) and running biomechanics. A systematic search on four electronic databases (Pubmed, WOS, Medline and Scopus) was conducted on 26 May 2021. Twelve studies met the inclusion criteria and were included. Standardised mean difference with 95% confidence intervals (CI) between footwear with increased LBS vs. non-increased LBS conditions and effect sizes were calculated. To assess the potential effects of moderator variables (type and length plate, increased LBS, shoe mass and running speed) on the main outcome variable (i.e. RE), subgroup analyses were performed. Increased LBS improved RE (SMD = -0.43 [95% CI -0.58, -0.28], Z = 5.60, p < 0.001) compared to non-increased LBS. Significant increases of stride length (SMD = 0.29 [95% CI 0.10, 0.49], Z = 2.93, p = 0.003) and contact time (SMD = 0.17 [95% CI 0.03, 0.31], Z = 2.32, p = 0.02) were found when LBS was increased. RE improved to a greater degree at higher running speeds with footwear with increased LBS. RE improved 3.45% with curve plate compared to no-plate condition without improvements with flat plate shoes. When shoe mass was matched between footwear with increased LBS vs. non-increased LBS conditions, RE improved (3.15%). However, when shoe mass was not controlled (experimental condition with ∼35 grams extra), a significant small improvement was found. These RE improvements appear along with an increase of stride length and contact time. Shoe mass, type of plate (flat or curve) and running speed should be taken into consideration when designing a shoe aimed at improving long-distance running performance.