Search

Five populations of a new dagger nematode species were recovered from natural grasslands and forests of north and northwest Iran, and described based upon morphological and molecular data in present paper. Xiphinema hyrcaniense n. sp. is characterized by 3.9–5.5 mm long females, having 102–142 μm long odontostyle, 64–88 μm long odontophore, guiding ring located at 115–147 μm distance from anterior end, two equally developed genital branches having crystalloids in tubular part of uteri and pseudo-Z-organ at their junction with pars dilatata uteri, short, rounded to dorsally more convex tail with a mucro or in few specimens, without it. Common males with 72–95 μm long spicules and four juvenile developmental stages. The new species is similar to six known species belonging to artificial morphospecies group 5, especially looking closest to X. montenegrinum in its general morphology, but could be separated by ontogenesis of tail shape and morphometric indices. In molecular phylogenetic analyses using partial large subunit, and internal transcribe spacer 1 ribosomal DNA (LSU D2-D3 and ITS1 rDNA) sequences, the new species formed a clade with X. cretense and Xiphinema sp. in LSU; and in ITS1 tree, with X. dentatum and X. paradentatum. © 2021, Koninklijke Nederlandse Planteziektenkundige Vereniging.

The genus Dolichodorus was discovered from a natural forest in Gilan province, north Iran, in association with an oak tree. The recovered population represents a new species, and was described and illustrated based upon morphological and molecular data, as D. rex n. sp. It is characterized by 2171–3275 μm long females having offset crown-like cephalic region with well sclerotized cephalic framework, raised perioral disc apparently with a small raised oral plate, 118.5, 130.0 μm long stylet, its conus ca. 55% of the total length and three posteriorly sloping knobs, 291–316 μm long pharynx with a short overlap, secretory-excretory pore at slightly variable position (one-two annuli posterior, or four annuli anterior to metacorpus base), vulva sunken in body, at %50 of the body length, vagina heavily sclerotized with two drop-like refractive pieces close to the vulval opening, 78–104 μm long tail with proximal convex conoid, and conical distal part with pointed to blunt tip; and a 2634 μm long male with 108.5 μm long stylet, 291 μm long pharynx, 20 μm long tail and 53 μm long spicules. In molecular phylogenetic analyses using partial sequences of the small, and D2-D3 expansion segments of the large subunit ribosomal DNA, the new species formed a clade with two other currently sequenced species of the genus in both phylogenies.

A Correction to this paper has been published: https://doi.org/ https://doi.org/10.1007/s10658-021-02272-1