1. Neurochemical factors associated with the antidepressant-like effect of flavonoid chrysin in chronically stressed mice
- Author
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Franciele Donato, Carlos Borges Filho, Renata Giacomeli, Cristiano Ricardo Jesse, Michelle S. Antunes, André Tiago Rossito Goes, Cristiane Luchese, Lucian Del Fabbro, Marcelo Gomes de Gomes, Silvane Souza Roman, Silvana Peterini Boeira, and Leandro Cattelan Souza
- Subjects
0301 basic medicine ,Serotonin ,medicine.medical_specialty ,Kynurenine pathway ,Adrenocorticotropic hormone ,Receptors, Corticotropin-Releasing Hormone ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Neurochemical ,Adrenocorticotropic Hormone ,Internal medicine ,medicine ,Animals ,Indoleamine-Pyrrole 2,3,-Dioxygenase ,Chronic stress ,Chrysin ,Kynurenine ,Caspase ,Flavonoids ,Pharmacology ,Behavior, Animal ,biology ,Tryptophan ,Neurochemistry ,Antidepressive Agents ,Tail suspension test ,030104 developmental biology ,Endocrinology ,chemistry ,Caspases ,biology.protein ,Cytokines ,Female ,Stress, Psychological ,030217 neurology & neurosurgery - Abstract
Chrysin is a flavonoid which is found in bee propolis, honey and various plants. Antidepressant-like effect of chrysin in chronically stressed mice was previously demonstrated by our group. Conversely, neurochemical factors associated with this effect require further investigations. Thus, we investigated the possible involvement of pro-inflammatory cytokines, kynurenine pathway (KP), 5-hydroxytryptamine (5-HT) metabolism and caspases activities in the effect of chrysin in mice exposed to unpredictable chronic stress (UCS). UCS applied for 28 days induced a depressive-like behavior, characterized by decrease in the time of grooming in the splash test and by increase in the immobility time in the tail suspension test. Oral treatment with chrysin (5 or 20mg/kg, 28 days), similarly to fluoxetine (10mg/kg, positive control), culminated in the prevention of these alterations. UCS elevated plasma levels of corticotropin-releasing hormone and adrenocorticotropic hormone, as well the tumor necrosis factor-α, interleukin-1β, interleukin-6 and kynurenine levels in the prefrontal cortex (PFC) and hippocampus (HP). UCS induced the decrease in the 5-HT levels in the HP and the increase in the indoleamine-2,3-dioxygenase, caspase 3 and 9 activities in the PFC and HP. Treatment with chrysin, similarly to fluoxetine, promoted the attenuation of these alterations occasioned by UCS. These results corroborated with the antidepressant potential of chrysin in the treatment of psychiatric diseases. Furthermore, this work indicated the association of pro-inflammatory cytokines synthesis, KP, 5-HT metabolism and caspases activities with the action exercised by chrysin in mice exposed to UCS.
- Published
- 2016