1. Treatment of inflamed ferret dental pulps with recombinant bone morphogenetic protein-7
- Author
-
Keni Gu and R. Bruce Rutherford
- Subjects
Pathology ,medicine.medical_specialty ,business.industry ,Chemistry ,Dentistry ,medicine.disease ,Bone morphogenetic protein ,law.invention ,Bone morphogenetic protein 7 ,stomatognathic diseases ,medicine.anatomical_structure ,stomatognathic system ,law ,In vivo ,Dentinogenesis ,Recombinant DNA ,medicine ,Dentin ,Pulp (tooth) ,Pulpitis ,business ,General Dentistry - Abstract
Recombinant human BMP-7 (bone morphogenetic protein-7, osteogenic protein-1) is osteogenic, dentinogenic and cementogenic when implanted into the appropriate tissue in vivo. However, most studies characterizing the induction of these tissues have implanted BMP-7 into freshly surgerized, clinically healthy tissues. To determine if BMP-7 is dentinogenic in inflamed dental pulps, we applied BMP-7 to inflamed ferret pulps. A single application of 5 microg of a commercial preparation of lipopolysaccharide (LPS) from Salmonella typhimurium directly to the coronal pulp induced a reversible mixed inflammatory exudate of moderate intensity within 3 d. Treatment with a single application of 2.5, 7.5 or 25 microg recombinant human BMP-7/mg collagen (2 mg total mass/tooth) induced reparative dentinogenesis in controls but not LPS treated dental pulps. These data reveal that a single application of up to 50 microg/tooth of exogenous recombinant BMP-7 is insufficient to induce reparative dentinogenesis in ferret teeth with reversible pulpitis. Given that pulp cells in the inflamed tissues likely retain the capacity to respond to exogenous BMP-7, it is possible that insufficient active recombinant protein is available to induce tissue formation in experimentally inflamed dental pulps.
- Published
- 2000