1. Enhancing surgical outcomes: accurate identification and removal of prostate cancer with B7-H3-targeted NIR-II molecular imaging.
- Author
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Tian, Yaqi, Shen, Hongliang, Li, Lingbing, Jia, Xiaohua, Liu, Jin, Hu, Zhenhua, Wang, Liang, and Tian, Jie
- Subjects
PROSTATE ,PROSTATE cancer ,ENDORECTAL ultrasonography ,BENIGN prostatic hyperplasia ,SURGICAL margin ,MOLECULAR probes ,SIGNAL-to-noise ratio - Abstract
Purpose: One of the main reasons for prostate cancer (PCa) recurrence is the difficulty in identifying and removing cancerous lesions during surgery. Accurately localizing and excising cancerous tissue remains a significant challenge. The second near-infrared window (NIR-II, 1000–1700 nm) fluorescence offers enhanced resolution, a high signal-to-noise ratio, and the potential for deeper tissue penetration. However, this technology is not currently employed for intraoperative imaging of PCa. This study aims to construct a new NIR-II probe targeting B7-H3 (Ab
B7−H3 -800CW) for accurate intraoperative identification and resection of PCa. Methods: Based on the differential expression of B7-H3 in PCa, we designed a novel imaging probe to accurately identify and guide the resection of preclinical PCa models and ex vivo human PCa tissues using NIR-II fluorescence imaging technology. Results: Analyzing tissue samples from 60 clinical cases of PCa, along with benign prostatic hyperplasia and normal prostate tissue from 22 cases, we observed a significant difference in B7-H3 protein expression levels (P < 0.001). Subcutaneous and orthotopic mouse models of PCa were imaged using NIR-II fluorescence after AbB7−H3 -800CW injection, showing promising results with successful tumor targeting and high-contrast images achieved within 24–48 h post-injection. The imaging also enabled the detection of occult PCa lesions approximately 1 mm in diameter. In addition, imaging analysis of human PCa and adjacent tissues using AbB7−H3 -800CW incubation revealed that cancer tissues exhibited a significantly higher fluorescence intensity than adjacent tissues (P < 0.05), which was conducive to the evaluation of tumor resection margin in vitro. Conclusion: The findings revealed that B7-H3 was a compelling imaging target for PCa. The AbB7−H3 -800CW molecular imaging probe is capable of accurately identifying PCa lesions and guiding their removal. This approach can potentially reduce the rate of surgical margins under NIR-II fluorescence guidance. [ABSTRACT FROM AUTHOR]- Published
- 2024
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