1. Structure-kinetics relationships of Capadenoson derivatives as adenosine A 1 receptor agonists
- Author
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Laura H. Heitman, Adriaan P. IJzerman, Julien Louvel, Tamara A. M. Mocking, Marjolein Soethoudt, Thea Mulder-Krieger, Eelke B. Lenselink, and Dong Guo
- Subjects
Structure-affinity relationships ,Models, Molecular ,Stereochemistry ,Kinetics ,Aminopyridines ,Extracellular loops ,Structure-Activity Relationship ,Adenosine A1 receptor ,Capadenoson ,Drug Discovery ,Humans ,Homology modeling ,Structure-kinetics relationships ,Pharmacology ,Ligand efficiency ,Dose-Response Relationship, Drug ,Molecular Structure ,Receptor, Adenosine A1 ,Chemistry ,Drug candidate ,Residence time ,Organic Chemistry ,General Medicine ,Receptor–ligand kinetics ,Adenosine A1 Receptor Agonists ,Thiazoles ,Adenosine A(1) receptor ,Dissociation kinetics - Abstract
We report the synthesis and biological evaluation of new derivatives of Capadenoson, a former drug candidate that was previously advanced to phase IIa clinical trials. 19 of the 20 ligands show an affinity below 100 nM at the human adenosine A1 receptor (hA1AR) and display a wide range of residence times at this target (from approx. 5 min (compound 10) up to 132 min (compound 5)). Structure-affinity and structure-kinetics relationships were established, and computational studies of a homology model of the hA1AR revealed crucial interactions for both the affinity and dissociation kinetics of this family of ligands. These results were also combined with global metrics (Ligand Efficiency, cLogP), showing the importance of binding kinetics as an additional way to better select a drug candidate amongst seemingly similar leads.
- Published
- 2015