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Mono‐ and binuclear benzimidazole based half‐sandwich organoruthenium(II) compounds; [Run(triazolateCOOCH3,COOCH3)n(η6‐p‐Cym)nL]0/+/2+ (n = 1, L = LCH2CH3 (7) and HLSO3H (8); n = 2, L = LDTP (9)) were synthesized by [3+2] free catalyzed cycloaddition reaction of azide complexes 4–6 with electron‐poor alkyne dimethyl acetylene dicarboxylate. In comparison with the parent [RunCln(η6‐p‐Cym)nL]0/+/2 complexes (1–3), the lysozyme binding affinity of the corresponding triazolate compounds 7–9 was investigated by electrospray ionization mass spectrometry. Complexes bearing benzimidazole ligand with an alkylated sulfonate side chain (2 and 8) are able to bind lysozyme noncovalently, which have not been clearly seen by other investigated Ru(II) complexes. The complexes were assessed for their potential antimicrobial activity against some representative microbes. Complex 9 exhibits antifungal activity against C. albicans (MIC = 24 nm) and C. neoformans (MIC = 12 nm), perfect blood compatibility as well as no toxicity to non‐malignant cell line (human embryonic kidney cells (HEK293). [ABSTRACT FROM AUTHOR]

In this work, facial tricarbonylmanganese(I) complexes [Mn(bpeaCH2C6H4R)(CO)3]PF6 incorporating a functionalized 2,2-bis(pyrazolyl)ethylamine (bpea) ligand with R = I, C≡CH, and CHO have been explored for their utility in bioorthogonal coupling to carrier peptides bearing N-terminal alkyne, azide, and aminoxy residues. As a model system, the transforming growth factor β-recognizing (TGF-β) peptide sequence R′-Leu-Pro-Leu-Gly-Asn-Ser-His-OH was used in which R′ is the reactive group complementary to the metal complex functionality. The use of catalyst-free oxime ligation gave the most stable conjugate with no degradation observed by HPLC over 96 h even after repeated freeze-thaw cycles. Both the parent complex as well as the functionalized peptide were investigated for photoactivated CO delivery to heme proteins by using the myoglobin assay and found to have essentially identical release properties. This work has established a new strategy for the conjugation of photoactivatable CO-releasing molecules (PhotoCORMs) to biological carrier systems. [ABSTRACT FROM AUTHOR]