1. CD40L-expressing CD8 T cells prime CD8alpha(+) DC for IL-12p70 production.
- Author
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Wong KL, Lew FC, MacAry PA, and Kemeny DM
- Subjects
- Animals, Cell Communication, H-2 Antigens immunology, Interferon-gamma physiology, Mice, Mice, Inbred C57BL, CD40 Ligand physiology, CD8 Antigens physiology, CD8-Positive T-Lymphocytes physiology, Dendritic Cells metabolism, Interleukin-12 biosynthesis
- Abstract
CD8alpha(+) DC are implicated as the principle DC subset for cross-presentation and cross-priming of cytotoxic CD8 T cell responses. In this study, we demonstrate another unique facet of the CD8alpha(+) DC and CD8 T cell relationship, by showing that CD8 T cells reciprocally activate CD8alpha(+) DC, but not CD8alpha(-) DC, for IL-12p70 production, the key Th1-promoting cytokine. This effect was observed during an antigen-specific interaction between DC and activated CD8 T cells, along with secondary TLR stimulation of DC by LPS. Activated CD8 T cells use a combination of IFN-gamma and CD40L, which is rapidly up-regulated post-stimulation, to prime DC for IL-12p70 production during an antigen-specific response. Our results suggest that the interaction between CD8alpha(+) DC and antigen-primed CD8 T cells may form an important component of Th1-mediated immunity through the induction of IL-12p70.
- Published
- 2008
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