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Your search keyword '"Kishihara, Kenji"' showing total 14 results
Start Over Author "Kishihara, Kenji" Journal european journal of immunology
14 results on '"Kishihara, Kenji"'

4. T lymphocyte development in p56lck deficient mice: allelic exclusion of the TcR β locus is incomplete but thymocyte development is not restored by TcR β or TcR αβ transgenes

Author

Wallace, Valerie A., primary, Kawai, Kazuhiro, additional, Levelt, Christiaan N., additional, Kishihara, Kenji, additional, Molina, Thierry, additional, Timms, Emma, additional, Pircher, Hanspeter, additional, Penninger, Josef, additional, Ohashi, Pamela S., additional, Eichmann, Klaus, additional, and Mak, Tak W., additional

Published
1995
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13. Mechanism of murine Vγ1<SUP>+</SUP> γ δ T cell-mediated innate immune response against Listeria monocytogenes infection

Author

Matsuzaki, Goro, Yamada, Hisakata, Kishihara, Kenji, Yoshikai, Yasunobu, and Nomoto, Kikuo

Abstract

Murine γ δ T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the γ δ T cell-mediated response against L. monocytogenes infection. γ δ T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-γ in vitro in response to L. monocytogenes-infected spleen cells. The IFN-γ production was abrogated by depletion of Vγ1+ γ δ T cells. IFN-γ production of the Vγ1+ γ δ T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR γ δ monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vγ1+ γ δ T cells of naive mice also produced IFN-γ in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vγ1+ γ δ T cells of naive mice as well as those of L. monocytogenes-infected mice although naive α β T cells lack IL-12R expression. All the results suggest that the Vγ1+ γ δ T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-γ in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation.

Published
2002
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14. Mechanism of murine Vgamma1+ gamma delta T cell-mediated innate immune response against Listeria monocytogenes infection.

Author

Matsuzaki G, Yamada H, Kishihara K, Yoshikai Y, and Nomoto K

Subjects
Animals, Antibodies, Monoclonal immunology, Antibodies, Monoclonal pharmacology, Cells, Cultured drug effects, Cells, Cultured immunology, Immunity, Cellular, Immunity, Innate, Immunoglobulin Fab Fragments immunology, Immunoglobulin Fab Fragments pharmacology, Interferon-gamma metabolism, Interleukin-12 pharmacology, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Receptors, Antigen, T-Cell, gamma-delta genetics, Receptors, Interleukin biosynthesis, Receptors, Interleukin-12, Spleen cytology, T-Lymphocyte Subsets drug effects, T-Lymphocyte Subsets metabolism, Listeria monocytogenes immunology, Listeriosis immunology, Receptors, Antigen, T-Cell, gamma-delta immunology, T-Lymphocyte Subsets immunology
Abstract

Murine gamma delta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gamma delta T cell-mediated response against L. monocytogenes infection. gamma delta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells. The IFN-gamma production was abrogated by depletion of Vgamma1+ gamma delta T cells. IFN-gamma production of the Vgamma1+ gamma delta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gamma delta monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vgamma1+ gamma delta T cells of naive mice also produced IFN-gamma in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vgamma1+ gamma delta T cells of naive mice as well as those of L. monocytogenes-infected mice although naive alpha beta T cells lack IL-12R expression. All the results suggest that the Vgamma1+ gamma delta T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-gamma in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation.

Published
2002
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