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Your search keyword '"FERLAZZO, V."' showing total 3 results
Start Over Author "FERLAZZO, V." Journal european journal of immunology
3 results on '"FERLAZZO, V."'

1. Reciprocal stimulation of gammadelta T cells and dendritic cells during the anti-mycobacterial immune response

Author

Viviana Ferlazzo, Serena Meraviglia, Cesira T. Bonanno, Juraj Ivanyi, Nadia Caccamo, Francesco Dieli, Carmela La Mendola, Alfredo Salerno, Guido Sireci, DIELI F, CACCAMO N, MERAVIGLIA S, IVANYI J, SIRECI G, BONANNO CT, FERLAZZO V, LA MENDOLA C, and SALERNO A

Subjects
Male, Immunology, Antigen presentation, Enzyme-Linked Immunosorbent Assay, Biology, CD8-Positive T-Lymphocytes, Lymphocyte Activation, Interleukin 21, Interferon-gamma, Mice, T-Lymphocyte Subsets, Immunology and Allergy, Cytotoxic T cell, Animals, Tuberculosis, IL-2 receptor, Antigen-presenting cell, Mice, Knockout, CD28, Cell Differentiation, Receptors, Antigen, T-Cell, gamma-delta, Dendritic Cells, Mycobacterium tuberculosis, Acquired immune system, Natural killer T cell, Cytotoxicity Tests, Immunologic, Interleukin-12, Coculture Techniques, Cell biology, Specific Pathogen-Free Organisms, Mice, Inbred C57BL, Female
Abstract

Gammadelta T cells and dendritic cells (DC) are two distinct cell types of innate immunity that participate in early phases of immune response against Mycobacterium tuberculosis infection. Here we show that a close functional relationship exists between these cell populations. Using an in vitro coculture system, Vgamma1 T cells from Tcrb(-/- )mice were found to be activated by DC infected in vitro with BCG, as indicated by the elevated CD69 expression, IFN-gamma secretion and cytotoxic activity. This activation process was due to a non-cognate mechanism since it required neither cell to cell contact nor interaction between the TCR and a specific antigen, but was mediated by DC-derived IL-12. Reciprocally, Vgamma1 T cells provided a key cytokine, IFN-gamma, which increased IL-12 production by BCG-infected DC. Moreover, exposure of BCG-infected DC to Vgamma1 T cells conditioned the former to prime a significantly stronger anti-mycobacterial CD8 T cell response. Consequently, stimulation of gammadelta T cells and their non-cognate interaction with DC could be applied as an immune adjuvant strategy to optimize vaccine-induced CD8 T cell immunity.

Published
2004

2. Differential requirements for antigen or homeostatic cytokines for proliferation and differentiation of human Vgamma9Vdelta2 naive, memory and effector T cell subsets.

Author

Caccamo N, Meraviglia S, Ferlazzo V, Angelini D, Borsellino G, Poccia F, Battistini L, Dieli F, and Salerno A

Subjects
Cell Differentiation, Cells, Cultured, Homeostasis, Humans, Interleukin-15 pharmacology, Leukocyte Common Antigens analysis, Receptors, Interleukin-15, Receptors, Interleukin-2 analysis, Tumor Necrosis Factor Receptor Superfamily, Member 7 analysis, Antigens immunology, Cytokines pharmacology, Immunologic Memory, Lymphocyte Activation, Receptors, Antigen, T-Cell, gamma-delta analysis, T-Lymphocyte Subsets immunology
Abstract

We have compared four human subsets of Vgamma9Vdelta2 T cells, naive (T(naive), CD45RA(+)CD27(+)), central memory (T(CM), CD45RA(-)CD27(+)), effector memory (T(EM), CD45RA(-)CD27(-)) and terminally differentiated (T(EMRA), CD45RA(+)CD27(-)), for their capacity to proliferate and differentiate in response to antigen or homeostatic cytokines. Cytokine responsiveness and IL-15R expression were low in T(naive) cells and progressively increased from T(CM) to T(EM) and T(EMRA) cells. In contrast, the capacity to expand in response to antigen or cytokine stimulation showed a reciprocal pattern and was associated with resistance to cell death and Bcl-2 expression. Whereas antigen-stimulated cells acquired a T(CM) or T(EM) phenotype, IL-15-stimulated cells maintained their phenotype, with the exception of T(CM) cells, which expressed CD27 and CD45RA in various combinations. These results, together with ex vivo bromodeoxyuridine incorporation experiments, show that human Vgamma9Vdelta2 memory T cells have different proliferation and differentiation potentials in vitro and in vivo and that T(EMRA) cells are generated from the T(CM) subset upon homeostatic proliferation in the absence of antigen.

Published
2005
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3. Reciprocal stimulation of gammadelta T cells and dendritic cells during the anti-mycobacterial immune response.

Author

Dieli F, Caccamo N, Meraviglia S, Ivanyi J, Sireci G, Bonanno CT, Ferlazzo V, La Mendola C, and Salerno A

Subjects
Animals, CD8-Positive T-Lymphocytes microbiology, Cell Differentiation immunology, Coculture Techniques, Cytotoxicity Tests, Immunologic, Dendritic Cells cytology, Dendritic Cells microbiology, Enzyme-Linked Immunosorbent Assay, Female, Interferon-gamma immunology, Interleukin-12 immunology, Lymphocyte Activation immunology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Specific Pathogen-Free Organisms, T-Lymphocyte Subsets microbiology, Tuberculosis microbiology, Tuberculosis prevention & control, CD8-Positive T-Lymphocytes immunology, Dendritic Cells immunology, Mycobacterium tuberculosis immunology, Receptors, Antigen, T-Cell, gamma-delta immunology, T-Lymphocyte Subsets immunology, Tuberculosis immunology
Abstract

Gammadelta T cells and dendritic cells (DC) are two distinct cell types of innate immunity that participate in early phases of immune response against Mycobacterium tuberculosis infection. Here we show that a close functional relationship exists between these cell populations. Using an in vitro coculture system, Vgamma1 T cells from Tcrb(-/- )mice were found to be activated by DC infected in vitro with BCG, as indicated by the elevated CD69 expression, IFN-gamma secretion and cytotoxic activity. This activation process was due to a non-cognate mechanism since it required neither cell to cell contact nor interaction between the TCR and a specific antigen, but was mediated by DC-derived IL-12. Reciprocally, Vgamma1 T cells provided a key cytokine, IFN-gamma, which increased IL-12 production by BCG-infected DC. Moreover, exposure of BCG-infected DC to Vgamma1 T cells conditioned the former to prime a significantly stronger anti-mycobacterial CD8 T cell response. Consequently, stimulation of gammadelta T cells and their non-cognate interaction with DC could be applied as an immune adjuvant strategy to optimize vaccine-induced CD8 T cell immunity.

Published
2004
Full Text
View/download PDF

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