1. Mesenchymal stromal cells‐derived small extracellular vesicles modulate DC function to suppress Th2 responses via IL‐10 in patients with allergic rhinitis
- Author
-
Peng, Ya‐Qi, Wu, Zi‐Cong, Xu, Zhi‐Bin, Fang, Shu‐Bin, Chen, De‐Hua, Zhang, Hong‐Yu, Liu, Xiao‐Qing, He, Bi‐Xin, Chen, Dong, Akdis, Cezmi A, Fu, Qing‐Ling, University of Zurich, and Fu, Qing‐Ling
- Subjects
2403 Immunology ,Immunology ,Cell Differentiation ,Mesenchymal Stem Cells ,610 Medicine & health ,Dendritic Cells ,Rhinitis, Allergic ,Extracellular Vesicles ,10183 Swiss Institute of Allergy and Asthma Research ,Leukocytes, Mononuclear ,2723 Immunology and Allergy ,Humans ,Immunology and Allergy ,Cells, Cultured - Abstract
Mesenchymal stromal cells (MSCs) are well known for their immunoregulatory roles on allergic inflammation particularly by acting on T cells, B cells, and dendritic cells (DCs). MSC-derived small extracellular vesicles (MSC-sEV) are increasingly considered as one of the main factors for the effects of MSCs on immune responses. However, the effects of MSC-sEV on DCs in allergic diseases remain unclear. MSC-sEV were prepared from the induced pluripotent stem cells (iPSC)-MSCs by anion-exchange chromatography, and were characterized with the size, morphology, and specific markers. Human monocyte-derived DCs were generated and cultured in the presence of MSC-sEV to differentiate the so-called sEV-immature DCs (sEV-iDCs) and sEV-mature DCs (sEV-mDCs), respectively. The phenotypes and the phagocytic ability of sEV-iDCs were analyzed by flow cytometry. sEV-mDCs were co-cultured with isolated CD4
- Published
- 2022