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Tropical Cichlid fishes (Tilapia mariae) and guinea pigs are able to produce antibodies to ovine anterior pituitary hormones. The immune response in fish is weaker than in mammals. Antisera of both animal groups are suitable specific ones but the fish immune system does not react very sensitively to small contaminations of the antigens. Electrophoretical examinations of anterior pituitary hormones (LTH, STH, FSH, LH and TSH) on gelatinized cellulose acetate strips show that except STH all the other hormones consist of several components. This is confirmed by immunological methods. There is some evidence that LH occurs in several molecular forms or components. Cross reactions and immunoelectrophoreses show a distinct separation of the immunological properties of the protein LTH and its antibody resp. and the glycoproteids FSH, LH, TSH and their antibodies. The protein STH and its homologous antibody, however, precipitate with all other hormones and their antibodies tested, except TSH. This possibly depends on the contamination of these hormones one with another.