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This article presents a report on otitis externa due to Trichoderma longibrachiatum. Fungi are occasional pathogens in otitis externa, mainly in chronic infections. Aspergillus, the main pathogens of otomycosis, are responsible for 54-80% of cases. The researchers report here a well-documented case of otitis externa due to Trichoderma longibrachiatum. The identification was confirmed by direct sequencing of a fragment coding for the ribosomal DNA. This case and other reports in the literature illustrate the wide spectrum of fungal pathogens isolated in otomycosis and the growing importance of molecular biology in the field of identification in medical mycology.