1. ADAM-HCV, a new-concept diagnostic assay for antibodies to hepatitis C virus in serum.
- Author
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Minenkova, Olga, Gargano, Nicola, De Tomassi, Amedeo, Bellintani, Francesca, Pucci, Andrea, Fortugno, Paola, Fuscaldi, Elena, Pessi, Antonello, Rapicetta, Maria, Miceli, Michela, Iudicone, Paola, Cortese, Riccardo, Felici, Franco, and Monaci, Paolo
- Subjects
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BIOLOGICAL assay , *HEPATITIS C virus - Abstract
We screened phage libraries using sera from noninfected individuals and patients infected by hepatitis C virus (HCV). By applying different selection and maturation strategies, we identified a wide collection of efficient phage-borne ligands for HCV-specific antibodies. The selected ligands retained their antigenic properties when expressed as multimeric synthetic peptides. Peptides that mimic several immunodominant epitopes of the virus were used to develop a novel type of diagnostic assay which efficiently detects antibodies to HCV in serum. This type of analysis provides a conclusive diagnosis for many patients identified as indeterminate according to presently available serological assays. [ABSTRACT FROM AUTHOR]
- Published
- 2001
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