1. Isolation of a Strong Suppressor of Nonsense Mutations in <em>Bacillus subtilis</em>.
- Author
-
Mellado, Rafael P., Viñuela, Eladio, and Salas, Margarita
- Subjects
BACILLUS subtilis ,ESCHERICHIA coli ,OCHER ,GENES ,STOICHIOMETRY ,PROTEINS - Abstract
By treatment of Bacillus subtilis MO-101-P spoA
- met thr- su- with ethyl methanesulfonate, a strong suppressor strain of nonsense mutations, B. subtilis MO-101-P spoA- [met- ]+ thr- su+44 , was isolated. This strain does not suppress phage Φ29 mutant susB47, selected on a B. subtilis strain containing the su+3 suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the su+3 and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. The experiments shown in this paper suggest that strain su+44 contains an amber and strain su+3 an ochre suppressor. [ABSTRACT FROM AUTHOR]- Published
- 1976
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