1. Cell Envelope Composition of Escherichia coli K12: A Comparison of the Cell Poles and the Lateral Wall
- Author
-
Uli Schwarz, Ernest W. Goodell, and Ron M. Teather
- Subjects
Chromatography, Paper ,Peptidoglycan ,Biology ,Cell Fractionation ,medicine.disease_cause ,Biochemistry ,chemistry.chemical_compound ,Cell Wall ,Deoxy Sugars ,Ketoses ,Centrifugation, Density Gradient ,Escherichia coli ,medicine ,Inner membrane ,NADH, NADPH Oxidoreductases ,Phospholipids ,Phosphatidylglycerol ,Strain (chemistry) ,Proteins ,Sugar Acids ,Culture Media ,Succinate Dehydrogenase ,Membrane ,chemistry ,Cytoplasm ,Glycerophosphates ,Isotope Labeling ,Biophysics ,Electrophoresis, Polyacrylamide Gel ,Chromatography, Thin Layer ,Cell envelope ,Bacterial outer membrane ,Subcellular Fractions - Abstract
Minicells and filaments of Escherichia coli strain P678–54 provide a convenient means of preparing cell-envelope fractions that are, respectively, greatly enriched or reduced in material derived from the cell poles. The inner (cytoplasmic) membrane, outer membrane, and sacculus were prepared both from minicells and from filaments purified from a single culture. One protein component and phosphatidylglycerol were found to be enriched in minicell inner membrane.
- Published
- 1974