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We describe a sensitive immunological procedure for the detection of specific proteins in total cell extracts and for the comparison of antigenically related polypeptides. Proteins are fractionated in polyacrylamide gels and transferred electrophoretically to diazophenylthioether paper, to which they bind covalently. Specific proteins are identified by incubation with specific antibody and ⊃125 -labeled protein A from Staphylococcus aureus, followed by autoradiography. High-resolution separation of proteins prior to transfer is achieved by polyacrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate or by nonequilibrium pH gradient electrophoresis, followed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Further information can be obtained by limited enzymatic proteolysis of the proteins in the gel following polyacryllamide gel electrophoresis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by gel electrophoresis at right angles to the first gel. We show the application of this technique to the detection and comparison in extracts from infected cells of proteins related immunologically to the simian virus 40 capsid proteins VP1 and VP3. [ABSTRACT FROM AUTHOR]

In human breast cancer MCF-7 and MCF-7ras cells, we demonstrated that whereas insulin had a mitogenic effect on both cell lines, fibroblast growth factor-2 (FGF-2) had opposite effects, stimulating MCF-7 and inhibiting MCF-7ras cell proliferation. The inhibitory signal induced by FGF-2 was related to sustained mitogen-activated protein kinase (MAPK) activation in MCF-7ras cells, while transient MAPK activation was associated with MCF-7 cell proliferation. FGF-2 was further used in combination with insulin or cAMP. In MCF-7 cells, insulin and cAMP reversed the mitogenic effect of FGF-2. In MCF-7ras cells, insulin did not modify the inhibitory effect of FGF-2, but cAMP markedly enhanced it. These effects were also associated with an increased level and duration of MAPK activation. PD98056 abolished the effect of FGF-2 on DNA synthesis in both cell lines, demonstrating that the dual effect of FGF-2 on cell proliferation is dependent on the activity of the extracellular-signal-regulated kinase 1 and 2 (ERK1/2) signalling pathway. [ABSTRACT FROM AUTHOR]

Carotenoid-depleted fruit flies, Drosophila melanogaster, were reared on yeast/glucose medium containing lipid-depleted white corn grits and cholesterol. After rearing for more than a year, the yield of flies remained constant and the content of 3-hydroxyretinal in a head was three logarithmic units less than that of normal flies reared on medium containing yellow corn grits. When all-trans retinal was supplied as the sole source of retinoids, the flies formed and accumulated all-trans 3-hydroxyretinal in the dark. To examine the metabolic pathway to produce (3S)-3-hydroxyretinal in Drosophila, all-trans retinal was supplemented for two hours to carotenoid-depleted flies in the dark, and the subsequent changes in the composition of 3-hydroxyretinal enantiomers were analyzed using a chiral column on HPLC. The results indicated initial formation of (3R)-3-hydroxyretinal followed by isomerization into the 3S enantiomer. In another set of experiments, the membrane fraction was obtained from the head homogenate of retinoid-depleted flies and an in vitro assay of 3-hydroxyretinal formation from retinal was performed. The 3-hydroxyretinal produced was the 3R enantiomer, supporting the result obtained from the in vivo experiment whereby (3S)-3-hydroxyretinal is produced from retinal via (3R)-3-hydroxyretinal. Addition of NADPH enhanced 3-hydroxyretinal formation and the presence of carbon monoxide inhibited it, suggesting that hydroxylation at the C3 position of retinal occurred via the monooxygenase activity of cytochrome P-450. [ABSTRACT FROM AUTHOR]

This article presents a list of several research papers to be published in the European Journal of Biochemistry. Some of listed papers are "Changes in the Insulin-Sensitive Glycosyl-Phosphatidyl-Inositol Signalling System With Aging in Rat Hepatocytes," "Localization of the Tightly Bound Divalent-Cation-Dependcnt and Nucleotidc-Dependent Conformation Changes m G-Actin Using Limited Proteolytic Digestion" and "Structure of the Promoter Region of the Gene Encoding Cytochrome c Oxidase Subunit V in Dictyostelium."

Presents the titles and authors of articles to be published in 1994 issues of the "European Journal of Biochemistry."

Several studies indicate that in human choriogonadotropin the N-linked oligosaccharide at position 52 of the α-subunit is important for bioactivity. We have generated choriogonadotropin mutants in which the α52 glycosylation site is removed and the α and β subunits are covalently linked by intersubunit disulfide bonds. These mutants display wild-type receptor binding and bioactivity. Furthermore, we show that removal of the α52 sugar leads to instability of heterodimeric choriogonadotropin. Therefore, we conclude that the α52 oligosaccharide of choriogonadotropin is not involved in signal transduction, but in the stability of the heterodimer. [ABSTRACT FROM AUTHOR]

We have shown previously that the processing of asparagine-linked oligosaccharides in baby hamster kidney (BHK) cells is blocked only partially by the glucosidase inhibitors, 1-deoxynojidmycin and A'-methyl-1-deoxynojidmycin [Hughes, R, C, Foddy, L. & Bause, E. (1987) Biochem. J. 247, 537-544], Similar results are now reported for castanospermine, another inhibitor of processing glucosidases, and a detailed study of oligosaccharide processing in the inhibited cells is reported. In steady-state conditions the major endo-H-released oligosaccharides contained glucose residues but non-glycosylated oligosaccharides, including Man9GlcNAc to Man5GlcNAc, were also present. To determine the processing sequences occurring in the presence of castanospermine, BHK cells were pulse-labelled for various times with [³H]mannose and the oligosaccharide intermediates, isolated by gel filtration and paper chromatography, characterized by acetolysis and sensitivity to jack bean α-mannosidase. The data show that Glc3Man8GlcNAc2 is transferred to protein and undergoes processing to produce Glc3Man8GlcNAc2 and Glc3Man7GlcNAc2 as major species as well as a smaller amount of Man9GlcNAc2, Glucosidase-processed intermediates, Glc1Man8GlcNAc2 and Glc1Man7GlcNAc2, were also obtained as well as a Man7GlcNAc2 species derived from Glc1Man7GlcNAc2 and different from the Man7GlcNAc2 isomer formed in the usual processing pathway. No evidence for the direct transfer of non-glucosylated oligosacchaddes to proteins was obtained and we conclude that the continued assembly of complex-type glycans in castanospermine-inhibited BHK cells results from residual activity of processing glucosidases. [ABSTRACT FROM AUTHOR]

This study describes the structure of the N-linked oligosaccharide chains of bile-salt-dependent lipase isolated from the pancreatic juice of a normal donor. After hydrazinolysis, neutral sugar chains were separated from acidic chains by paper electrophoresis and were fractionated using serial column chromatography with immobilized lectins and Bio-Gel P-4 filtration. Structural analysis was performed by means of sequential glycosidase digestion and revealed that the neutral sugar chains are mainly of the biantennary complex type. Fucose residues were identified for some trimannosyl core structures and were α(1–6) or α(1–2) linked to the innermost GlcNAc residue and a terminal Gal residue, respectively. Sialyl residues were also involved in the oligosaccharide structures. Most of these structures have no linear N-acetyllactosamine repeats. Evidence from several approaches suggests that the sugar chains of the human pancreatic bile-salt-dependent lipase possess a blood-group-related antigenic determinant. [ABSTRACT FROM AUTHOR]

This article presents information on several forthcoming research papers relate to biochemistry. Some of the papers include: purification and characterization of rat-liver 3,4-dihydroxyphenylalanine decarboxylase; identification of novel arachidonic acid metabolites formed during prostaglandin biosynthesis in vitro; nucleotide sequence of the cDNA encoding the precursor for mitochondrial serine, integration of porin synthesized in vitro into outer mitochondrial membranes; structure of a synthetic pepsin inhibitor complexed with endothiapepsin, etc.

This article presents information on the forthcoming papers related to biochemistry. Some of the papers are Isolation and Characterization of Nucleic-Acid-Like Polymers Containing Glucose From Erich Ascites Tumor Cells by K. Kagawa and H. Kagawa, Abundant Messenger RNA Sequences Common to Transplanted Mouse Tumours of Different Tissue Origins by G. Mercier, M. Guillier and J. Harel and Proline Isomerization in Unfolded Ribonuclease A. The Equlibrium between Fast-Folding and Slow-Folding Species is independent of temperature by F. X. Schmid.

The article presents information on forthcoming articles and papers to be published in "European Journal of Biochemistry." The papers that have been listed are: "The Complete Nucleotide Sequence of a 23S RNA Gene From Tobacco Chloroplasts," "The Distribution of Ubiquinone-10 in Phospholipid Bilayers," "Relation of Oxidation of Long-Chain Fatty Acids to Gluconeogenesis in the Perfused Liver of the Guinea Pig," "Isolation and Characterization of New Collagens From Chick Cartilage," "Peroxisomal and Mitochondrial Carnitine Acetyltransferases in Alkane-Grown Yeast Candida Tropicalis," etc.

The article presents information on several forthcoming research papers, which will be published in the coming issues of the European Journal of Biochemistry. Some of the topics of the papers are: lipolysis, biosynthesis, electron transfer, molecular weights, etc.

Presents the forthcoming papers to be published in the "European Journal of Biochemistry." "Thioredoxin regenerates proteins inactivated by oxidative stress in endothelial cells," by M. R. Fernando, H. Nanri, S. Yoshitake, K. Nagata-Kuno and S. Minakami; "Tyrosine phosphorylation and the mechanism of signal transduction by the B-lymphocyte antigen receptor," by A. L. DeFranco; "Dissociation of F-actin induced by hydrostatic pressure," by C. R. S. Garcia, J. A. Amaral Jr., P. Abrahamsohn and S. Verjovski-Almelda; "Biological methane activation involves the intermediacy of carbon-cenetered radicals," by P. C. Wilkins, H. Dalton, I. D. Podmore, N. Deighton, and M. C. R. Symons.

Presents a list of forthcoming papers for the "European Journal of Biochemistry."

Lists the research papers to be published in the succeeding issues of the "European Journal of Biochemistry." "The structure of DNA junctions and their interaction with enzymes," by D.R. Duckett and colleagues; "CD4 gene transcription is transiently repressed during differentiation of myeloid cells to macrophage-like cells," by F.X. Pimentel-Muiñoz and colleagues; "Malonate decarboxylase of Malonomas rubra: a novel type of biotin-containing acetyl-enzyme," by H. Hilbi and colleagues.

Presents a lists of forthcoming papers for the journal "European Journal of Biochemistry." "Substance-p-related and Neurokinin-a-related Peptides From the Brain of the cod and Trout," by Jensen and J. M. Conlon; "Biochemical and Photochemical Properties of the Photophobic Receptors From Halobacterium Halobium and Natronobacterium Pharaonis," by B. Scharf, B. Pevec, and B. Hess; "Phosphatidylinositol-glycan-specific Phospholipase D is an Amphiphilic Glycoprotein That in Serum is Associated With High-Density Lipoproteins," by M. C. Hoener and U. Brodbeck.

Lists forthcoming papers on biochemistry, compiled as of April 1992. 'The Effect of Polylysine on Casein-kinase-2 Activity is Influenced by Both the Structure of the Protein/Peptide Substrates and the Subunit Composition of the Enzyme,' by F. Meggio, B. Boldyreff and O. Marin; 'Increased Steady-State Levels of Several Mitochondrial and Nuclear Gene Transcripts in Rat Hepatoma With a Low Content of Mitochondria,' by K. Luctakova and S. Kuzela; 'Phycobilisome Structure in the Cyanobacteria Mastiglocladus laminosus and Anabaena Sp. PCC 7120,' by M. Glauser and D.A. Bryant.

Presents the titles and authors of various research papers that will be published in the "European Journal of Biochemistry."

Lists papers scheduled for publication in the "European Journal of Biochemistry," as of February 2004. Subjects; Authors; Data presented; Experiments conducted.

Lists titles of research papers on biochemistry. "Redox control of catalysis in ATP-citrate lyase from rat liver"; "Construction and molecular dynamics of a soluble, enzymatically active form of the Escherichia coli penicillin-binding protein 5 and purification by dye chromatography"; "Studies on the variants of the protein toxins ricin and abrin".

Presents a listing of papers on biochemistry to be published in the "European Journal of Biochemistry.

Lists forthcoming papers on topics related to biochemistry.

Presents forthcoming papers published on the August 1, 1991 issue of the "European Journal of Biochemistry." "Purification and chemical modification of a phosphatidylinositol kinase from sheep brain," by G. Scholz, G. J. Barritt and F. Kwok; "Heat-shock response of rat hepatoma variant cells" by M. Baranyi, A. Hever-Szabo and A. Venetianer; "Structural studies of yeast iso-1 cytochrome c mutants by resonance Raman spectroscopy," by P. Hildebrandt, G. J. Pielak and R. J. P. Williams; "An amplified insect dihydrofolate reductase gene contains a single intron," by F. A. Shotkoski and A. M. Fallon.

Presents a list of research papers on biochemistry to be published in the "European Journal of Biochemistry."

Lists the research papers to be published in the succeeding issues of the "European Journal of Biochemistry." "Stimulation of non-selective cation channels providing Ca2+ influx into platelets by platelet-activating factor and other aggregation inducers," by P.V. Avdonin and colleagues; "Complete nucleotide sequence of the gene for human C1 inhibitor with an unusually high density of Alu elements," by P.E. Carter and colleagues.

Presents a list of papers scheduled to be published in forthcoming issues of the periodical "European Journal of Biochemistry," as of 1990.

Presents forthcoming papers for the "European Journal of Biochemistry". "A guanyloribonuclease mouse liver cytosol," by A. Pantazaki and colleagues; "Revised nonmenclature for eukaryotic DNA polymerases," by P.M.J. Burgers and colleagues; "The folding and solution conformation of penicillin G acylase," by C.D. Lindsay and colleagues.

Lists several upcoming papers related to microbiology published on the January 26, 1990 issue of the "European Journal of Biochemistry."

Cites several research papers on biochemistry. "Nematocysts (stinging capsules of Cnidaria) as Donnan-potential-dominated osmotic systems," by J. Weber; "Characterization of a high-affinity monoclonal antibody to calcineurin whose epitope defines a new structural domain of calcineurin A," by M.J. Hubbard and C.B. Klee; "Immunological detection of phytoene by bolulinum neurotoxin A - Contribution of various fragments to the intoxication process," by B. Poulain and colleagues.

Presents a list of forthcoming papers to be published in Volume 181, Number 1, April 1989 issue of the periodical "European Journal of Biochemistry."

Lists forthcoming papers on biochemistry, published in the June 1988 issue of the "European Journal of Biochemistry". 'Post-Translational Translocation of Polypeptides Across the Mammalian Endoplasmic Reticulum Membrane Is Dependent Upon Its Size and on the Ribosomes,' by T. Roitsch and L. Lehle; 'Characterization of the Inducible Polyamine Transporter in Bovine Lymphocytes,' by Y. Kakinuma, K.S. Montine, E.C. Henshaw and R. Panniers; 'Isolation and Characterization of a Highly Phosphorylated Troponin From Bovine Heart,' by N. Beier and K. Jacquet.

The article presents a list of topics that are will be published in the forthcoming issues of the "European Journal of Biochemistry." Some of them are Excision of a purinic and/or a pyridinic sites from DNA nucleolytical enzymes from rat brain, Purification and some properties of the corrinoid containing membrane protein from Methanobacterium thermoutotrophicum, Specific inhibition of simian virus 40 protein synthesis by heat and arsenite treatment and many other papers.

Lists papers to be published in the "European Journal of Biochemistry." Chemical modification of phenol hydroxylase; Fructose metabolism; Ester composition of carnitine; Structure of sidechain lipopolysaccharide from Erwinia amylovora T.

Presents upcoming research papers on biochemistry to be published in "European Journal of Biochemistry." Discussion of the molecular cloning and sequencing of a cDNA encoding the acyl carrier protein; Exploration of the primary structure of human thyroglobulin; Examination of the reversible activation of hydrogenase from Escherichia coli.

Presents a table depicting forthcoming papers in biochemistry, published in the April 1, 1987 issue of the "European Journal of Biochemistry."

Lists the titles and authors of papers that will be published in 1987 issues of the "European Journal of Biochemistry." Titles of articles and authors.

Presents the forthcoming research papers to be published in the "European Journal of Biochemistry."

The article presents name of some of the papers that will be published in the forthcoming issues of the "European Journal of Biochemistry." "A Small Diffusion Pore in the Outer Portion of Pseudomonas aeruginosa," "The Nicotine Acetylcholine Receptor and its Iron Channel," and many others.

Presents scientific papers to be published in the "European Journal of Biochemistry." Detection and distribution of myosin isozymes in vertebrate smooth muscle," by G. Beckers-Bleukx and G. Marechal; "Experimental evidence for multistability in a photobiochemical system"; "Reductive mobilization of ferritin iron," by F. Funk, J.-P. Lenders, R. R. Crichton and W. Schneider. "

Presents a list of forthcoming papers subsequent to the July 1985 issue of the "European Journal of Biochemistry."

Introduces the research papers to be published in the "European Journal of Biochemistry," succeeding the May 15, 1985. "Primer-independent abortive initiation by wheat-germ RNA polymerase B," by H. Mosig et al; "Protein structure and gene organization of mouse lactate dehydrogenase-A isozyme," S.S.-L. Li et al; "A model for intracellular complexation between gene-5 protein and bacteriophage fd DNA," by G.D. Bayer et al.

Presents the forthcoming papers to be published in the "European Journal of Biochemistry."

Lists papers due for publication in the "European Journal of Biochemistry" after the January 16, 1984 issue. "Labelling and cross-linking of Escherichia coli penicillin-binding proteins with bis-Beta-lactam antibiotics"; "Sorbitol dehydrogenase. The primary structure of the sheep-liver enzyme"; "Extensive variations and basic features in the alcohol dehydrogenase-sorbitol dehydrogenase family".

Presents several forthcoming papers published on the December 1, 1983 issue of the "European Journal of Biochemistry." "Purification and Properties of a Thiol Protease From Rat-Liver Nuclei"; Changes in Proteoglycan Compositiion of F9 Teratocarcinoma Cells Upon Differentiation"; "Supercoiling and the Mechanism of Restriction Endonucleases"; Identification of the Gene for DNA Helicase II of Escherichia Coli."

Presents a list of research papers on biochemistry to be published in the "European Journal of Biochemistry."

Presents the forthcoming papers subsequent to the August 1, 1983 issue of the "European Journal of Biochemistry."

Presents a list of research papers to be published in the "European Journal of Biochemistry."

Lists the titles of research papers to be published in the future issues of the "European Journal of Biochemistry." "Relationship between oligosaccharide-lipid synthesis and protein synthesis in mouse LM cells," by S.R. Grant and W.J. Lennarz; "Characterization of a cell line from Chinese Hamster Ovary Resistant to Uncoupler," by K.B. Freeman, et al; "The initiation of glycogen biosynthesis in rat heart," by M.L. Blumenfeld, et al.

The article presents a list of papers that are to be published in future issues of the European Journal of Biochemistry. Some of the articles are: Properties and Reconstruction of a Cytochrome Oxidase Deficient in Subunit III by T. Pentillä The Effects of Octylglucoside on the Interactions of Chloroplast Coupling factor 1 (CF1) With Adenine Nucleotides by U. Pick and S. Bassilian; Localization of Ellipticine Derivatives Interacting With Membranes. A Fluoroscence-Quenching Study by F. Terce, J.-F. Tocanne and G. Laneelle; and Improved Purification and Further Characterization of Acetyl-CoA Carboxylase From Cultures of Cells of Parsley by B. Egin-Bühler and J. Ebel.

The article presents information on various papers to be published in the "European Journal of Biochemistry." Some of them are, "Lipid Domains in Plasma Membranes From Rat Liver," by F. Schroeder, "Calcium Modulates Fatty Acid Dynamics in Rat Liver Plasma Membranes," by F. Schroeder, and C.S. Argilaga, and others.