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Purpose: Cortisol (C) and testosterone (T) are impacted significantly by prolonged endurance exercise with inverse responses. Increases in C are witnessed concurrently with decrements in T, possibly impacting recovery. This study was conducted to assess the dynamics of C and free T (fT) concentration and recovery time following an exhaustive endurance exercise session (EES).Methods: 12 endurance-trained males (X ± SD: VO2max 66.3±4.8 ml/kg/min, age 22.8 ± 3.1 years, body fat 11.0 ± 1.4 %, training 7.1 ± 3.2 years) completed a treadmill EES at ventilatory threshold (74.7 ± 4.6 % of VO2max; 96.9 ± 10.8 min). Basal blood C and fT were collected at baseline: -48, -24 h, and immediately before (0 h) the EES as well as immediately (+0 h), +24 h, +48 h, and +72 h after the EES. Blood glucose (G) was measured to confirm no undue influence on C. Statistically data were analyzed with repeated measures ANOVA (LSD post hoc).Results: C (nmol/L) increased significantly from -48 h (321 ± 59) to +0 h (701 ± 178) (p < 0.001), and displayed a baseline overshoot with +24 h (209 ± 67) being significantly lower than -48 and +0 h (p < 0.03). fT (pmol/L) decreased significantly from -48 h (161 ± 40) to +0 h (106 ± 38) (p < 0.01) and remained lower at +24 h (110 ± 33) and +48 h (129 ± 30) (p < 0.001). G remained stable throughout. A moderately negative correlation (r = -0.636, p < 0.026) was found between C and fT at +0 h.Conclusions: EES recovery may require 48 h for C and 72 h for fT to return to baseline values. Furthermore, C and fT were only correlated immediately post-exercise. Future research should perform more frequent measurements throughout time course. [ABSTRACT FROM AUTHOR]

L-Ornithine plays an important role in ammonia metabolism via the urea cycle. This study aimed to examine the effect of L-ornithine hydrochloride ingestion on ammonia metabolism and performance after intermittent maximal anaerobic cycle ergometer exercise. Ten healthy young adults (age, 23.8 ± 3.9 year; height, 172.3 ± 5.5 cm; body mass, 67.7 ± 6.1 kg) with regular training experience ingested L-ornithine hydrochloride (0.1 g/kg, body mass) or placebo after 30 s of maximal cycling exercise. Five sets of the same maximal cycling exercise were conducted 60 min after ingestion, and maximal cycling exercise was conducted after a 15 min rest. The intensity of cycling exercise was based on each subject's body mass (0.74 N kg(-1)). Work volume (watt), peak rpm (rpm) before and after intermittent maximal ergometer exercise and the following serum parameters were measured before ingestion, immediately after exercise and 15 min after exercise: ornithine, ammonia, urea, lactic acid and glutamate. Peak rpm was significantly greater with L-ornithine hydrochloride ingestion than with placebo ingestion. Serum ornithine level was significantly greater with L-ornithine hydrochloride ingestion than with placebo ingestion immediately and 15 min after intermittent maximal cycle ergometer exercise. In conclusion, although maximal anaerobic performance may be improved by L-ornithine hydrochloride ingestion before intermittent maximal anaerobic cycle ergometer exercise, the above may not depend on increase of ammonia metabolism with L-ornithine hydrochloride. [ABSTRACT FROM AUTHOR]

The purpose of this study was to determine whether sweat sodium concentration ([Na(+)](sweat)) during exercise in the heat differs between aerobically trained and untrained individuals. On three occasions, ten endurance-trained (Tr) and ten untrained (UTr) subjects (VO2peak = 4.0 ± 0.8 vs. 3.4 ± 0.7 L min(-1), respectively; P < 0.05) cycled in a hot-ventilated environment (36 ± 1°C; 25 ± 2% humidity, airflow 2.5 m s(-1)) at three workloads (i.e., 40, 60, and 80% VO2peak). Whole-body (SR(WB)) and back sweat rates (SR(BACK)) were measured. At the conclusion of the study, Na(+) in sweat and blood samples was analyzed to calculate Na(+) secretion and reabsorption rates. SR(WB) and SR(BACK) were highly correlated in Tr and UTr (r = 0.74 and 0.79, respectively; P < 0.0001). In both groups, SR(BACK) increased with the increases in exercise intensity (P < 0.05). Likewise, [Na(+)](sweat) increased with the exercise intensity in both groups (P < 0.05) and it tended to be higher in Tr than in UTr at 60 and 80% VO2peak (~22 mmol L(-1) higher; P = 0.06). However, when normalized for SR(BACK), [Na(+)](sweat) was not different between groups. In both groups, Na(+) secretion and reabsorption rates increased with the increases in SR(BACK) (P < 0.05). However, Na(+) reabsorption rate was lower in the Tr than in the UTr (mean slope = 48 vs. 82 ηmol cm(-2) min(-1); P = 0.03). In conclusion, using a cross-sectional study design, our data suggest that aerobic fitness level does not reduce sweat Na(+) secretion or enhance Na(+) reabsorption during prolonged exercise in the heat that induced high sweat rates. [ABSTRACT FROM AUTHOR]

The purpose of this study was to determine the influence of transiently elevated endogenous hormone concentrations during exercise on strength training adaptations. Nine subjects performed four unilateral strength training session per week on the elbow flexors for 11 weeks. During two of the weekly sessions, leg exercises were performed to acutely increase the systemic anabolic hormone concentration immediately before the exercises for one of the elbow flexors (L + A). On the two other weekly training sessions, the contralateral elbow flexors were trained without prior leg exercises (A). By randomizing one arm of the subjects to serve as a control and the other as experimental, both conditions have the same nutritional and genetic environment. Serum testosterone and growth hormone was significantly increased during the L - A training session, while no hormonal changes occurred in the A session. Both A and L + A increased 1RM in biceps curl, peak power in elbow flexors at 30 and 60% of 1RM, and muscle volume of the elbow flexors (p < 0.05). However, only L + A achieved increase in CSA at the part of the arm flexors with largest cross sectional area (p < 0.001), while no changes occurred in A. L + A had superior relative improvement in 1RM biceps curl and favorable muscle adaptations in elbow flexors compared to A (p < 0.05). In conclusion, performing leg exercises prior to arm exercises, and thereby increasing the levels of serum testosterone and growth hormone, induced superior strength training adaptations compared to arm training without acute elevation of hormones. [ABSTRACT FROM AUTHOR]

Carbohydrate stores within muscle are considered essential as a fuel for prolonged endurance exercise, and regimes for enhancing such stores have proved successful in aiding performance. This study explored the effects of a hyperglycaemic-hyperinsulinemic clamp performed 18 h previously on subsequent prolonged endurance performance in cycling. Seven male subjects, accustomed to prolonged endurance cycling, performed 90 min of cycling at ~65% VO(2max) followed by a 16-km time trial 18 h after a 2-h hyperglycemic-hyperinsulinemic clamp (HCC). Hyperglycemia (10 mM) with insulin infused at 300 mU/m(2)/min over a 2-h period resulted in a total glucose uptake of 275 g (assessed by the area under the curve) of which glucose storage accounted for about 73% (i.e. 198 g). Patterns of substrate oxidation during 90-min exercise at 65% VO(2max) were not altered by HCC. Blood glucose and plasma insulin concentrations were higher during exercise after HCC compared with control (p < 0.05) while plasma NEFA was similar. Exercise performance was improved by 49 s and power output was 10-11% higher during the time trial (p < 0.05) after HCC. These data suggest that carbohydrate loading 18 h previously by means of a 2-h HCC improves cycling performance by 3.3% without any change in pattern of substrate oxidation. [ABSTRACT FROM AUTHOR]

Cardiovascular deconditioning is known to occur in astronauts exposed to microgravity. Endothelial dysfunction at microcirculatory sites might contribute to cardiovascular deconditioning induced by weightlessness. Recent studies have reported changes in the morphology and gene expression of endothelial cells exposed to conditions of simulated microgravity. The present study was aimed at examining the effects of microgravity on the apoptosis of microvascular endothelial cells and the mechanism underlying these effects. We simulated a microgravity environment and found that microgravity induced microvascular endothelial cell apoptosis and that this effect was correlated with the downregulation of the PI3K/Akt pathway, increased expression of NF-κB, and depolymerization of F-actin. These findings may provide important insights into the origin of the adverse physiological changes occurring due to exposure to microgravity conditions. [ABSTRACT FROM AUTHOR]

Nutrition is an important co-factor in exercise-induced training adaptations in muscle. We compared the effect of 6 weeks endurance training (3 days/week, 1-2 h at 75% VO(2peak)) in either the fasted state (F; n = 10) or in the high carbohydrate state (CHO, n = 10), on Ca(2+)-dependent intramyocellular signalling in young male volunteers. Subjects in CHO received a carbohydrate-rich breakfast before each training session, as well as ingested carbohydrates during exercise. Before (pretest) and after (posttest) the training period, subjects performed a 2 h constant-load exercise bout (~70% of pretest VO(2peak)) while ingesting carbohydrates (1 g/kg h(-1)). A muscle biopsy was taken from m. vastus lateralis immediately before and after the test, and after 4 h of recovery. Compared with pretest, in the posttest basal eukaryotic elongation factor 2 (eEF2) phosphorylation was elevated in CHO (P < 0.05), but not in F. In the pretest, exercise increased the degree of eEF2 phosphorylation about twofold (P < 0.05), and values returned to baseline within the 4 h recovery period in each group. However, in the posttest dephosphorylation of eEF2 was negated after recovery in CHO, but not in F. Independent of the dietary condition training enhanced the basal phosphorylation status of Phospholamban at Thr(17), 5'-AMP-activated protein kinase α (AMPKα), and Acetyl CoA carboxylase β (ACCβ), and abolished the exercise-induced increase of AMPKα and ACCβ (P < 0.05). In conclusion, training in the fasted state, compared with identical training with ample carbohydrate intake, facilitates post-exercise dephosphorylation of eEF2. This may contribute to rapid re-activation of muscle protein translation following endurance exercise. [ABSTRACT FROM AUTHOR]

Both myofibroblast differentiation and extracellular matrix (ECM) deposition are essential components of scar formation in tendons, and hepatocyte growth factor (HGF) is reported to prevent fibrogenic responses in tendons. Matrix metalloproteinases-2(MMP-2) is also involved in the healing process in tendons. Whether HGF protects healed Achilles tendons from injury-induced scar formation and the mechanisms are unknown. Daily for 2 weeks after wounding, except for the non-surgical control group, the Achilles tendons in rats were locally injected with HGF (100 ng 50 μl(-1) per mouse) or phosphate-buffered saline (PBS). Histological examination showed HGF ameliorated disorganized collagen fibers caused by surgical incisions in rats. After transforming growth factor beta-1 (TGF-β1) induced fibrogenic responses in primary Achilles tendon fibroblasts in rats, HGF treatment for 24 h reduced α-smooth muscle actin (α-SMA) (0.60 ± 0.07-fold, P < 0.05) and type III collagen expression (0.39 ± 0.07-fold, P < 0.05). Moreover, HGF elevated MMP-2 expression (1.23 ± 0.11-fold, P < 0.05). The MMP-2 inhibitor, tissue inhibitors of metalloproteinase-1 (TIMP-1), partially blocked the inhibitory effects of HGF on α-SMA expression (from 0.60 ± 0.07-fold to 0.83 ± 0.07-fold, P < 0.05) and type III collagen expression (from 0.39 ± 0.06-fold to 0.86 ± 0.08-fold, P < 0.05). These results indicate HGF attenuates TGF-β1-induced fibrogenic responses in Achilles tendon, which was mediated by MMP-2. These results will aid in developing effective therapeutic approaches for the dysfunctional repair in Achilles tendons. [ABSTRACT FROM AUTHOR]

Optimal levels of membrane fluidity are essential for numerous cell functions including cell growth, solute transport and signal transduction. Since exercise enhances free radical production, our aim was to evaluate in healthy male subjects the effects of an acute bout of maximal and submaximal exercise on the erythrocyte membrane fluidity and its possible relation to the oxidative damage overproduction due to exercise. Subjects (n = 34) performed three cycloergometric tests: a continuous progressive exercise, a strenuous exercise until exhaustion and an acute bout of exercise at an intensity corresponding to 70% of maximal work capacity for 30 min. Venous blood samples were collected before and immediately after these exercises. Erythrocyte membrane fluidity was assessed by fluorescence spectroscopy. Plasma malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations and carbonyl content of plasmatic proteins were used as an index of lipid and protein oxidation, respectively. Exercise produced a dramatic drop in the erythrocyte membrane fluidity as compared to resting time, but this was not accompanied by significant changes in the plasmatic MDA and 4-HDA concentrations. The highest erythrocyte membrane rigidity was detected immediately after strenuous exercise until exhaustion was performed. Protein carbonyl levels were higher after exhaustive exercises than at rest. Continuous progressive and strenuous exercises until exhaustion, but not submaximal workload, resulted in a significant enhanced accumulation of carbonylated proteins in the plasma. These findings are consistent with the idea that exercise exaggerates oxidative damage, which may contribute, at least partially, to explain the rigidity in the membrane of the erythrocytes due to acute exercise. [ABSTRACT FROM AUTHOR]

This investigation reports the effects of caffeinated chewing gum on fatigue and hormone response during repeated sprint performance with competitive cyclists. Nine male cyclists (mean ± SD, age 24 ± 7 years, VO(2max) 62.5 ± 5.4 mL kg(-1) min(-1)) completed four high-intensity experimental sessions, consisting of four sets of 30 s sprints (5 sprints each set). Caffeine (240 mg) or placebo was administered via chewing gum following the second set of each experimental session. Testosterone and cortisol concentrations were assayed in saliva samples collected at rest and after each set of sprints. Mean power output in the first 10 sprints relative to the last 10 sprints declined by 5.8 ± 4.0% in the placebo and 0.4 ± 7.7% in the caffeine trials, respectively. The reduced fatigue in the caffeine trials equated to a 5.4% (90% confidence limit ±3.6%, effect size 0.25; ±0.16) performance enhancement in favour of caffeine. Salivary testosterone increased rapidly from rest (~53%) and prior to treatments in all trials. Following caffeine treatment, testosterone increased by a further 12 ± 14% (ES 0.50; ± 0.56) relative to the placebo condition. In contrast, cortisol concentrations were not elevated until after the third exercise set; following the caffeine treatment cortisol was reduced by 21 ± 31% (ES -0.30; ± 0.34) relative to placebo. The acute ingestion of caffeine via chewing gum attenuated fatigue during repeated, high-intensity sprint exercise in competitive cyclists. Furthermore, the delayed fatigue was associated with substantially elevated testosterone concentrations and decreased cortisol in the caffeine trials. [ABSTRACT FROM AUTHOR]

Acute exercise alters the surface expression of toll-like receptors (TLRs) and HLA.DR on blood monocytes, which could transiently compromise immunity. As serum factors might be responsible, we examined the effects of autologous post-exercise serum exposure on TLR2, TLR4 and HLA.DR expression on resting blood monocytes and their subtypes. Eight trained cyclists completed an ergometer 60 km time trial. PBMCs and serum were obtained before, immediately after and 1 h after exercise. TLR2, TLR4 or HLA.DR expression (gMFI) was determined on blood monocyte subtypes expressing combinations of CD14 and CD16 by flow cytometry, and on resting monocytes exposed to 50% autologous serum (pre, immediately after or 1 h after exercise) for 18 h in culture. Immediately after exercise, total monocyte expression of TLR2 and TLR4 increased by 41 and 27%, respectively, while HLA.DR expression was 39% lower than baseline. TLR2 and TLR4 was 53 and 84% greater 1 h after exercise, respectively, while HLA.DR was 48% lower. Changes in TLR2 and TLR4 expression occurred on the CD14(++bright)/CD16(+dim) monocyte subtype only, while HLA.DR expression changed on the CD14(+dim)/CD16(++bright) subtype. Serum did not affect monocyte TLR2 or TLR4 expression but 1 h post serum increased expression of HLA.DR on total monocytes and the CD14(+dim)/CD16(++bright) subtype, which was in contrast to the change observed at this time after exercise. We conclude that a bout of strenuous aerobic exercise alters the surface expression of TLR2, TLR4 and HLA.DR on blood monocytes and some of their subtypes, but these changes appear to be unrelated to blood serum factors. [ABSTRACT FROM AUTHOR]

The response of calf muscle strength, resting (R (bf)) and post-occlusive (PO(bf)) blood flow were investigated following 4 weeks resistance training with and without blood flow restriction in a matched leg design. Sixteen untrained females performed unilateral plantar-flexion low-load resistance training (LLRT) at either 25% (n = 8) or 50% (n = 8) one-repetition maximum (1 RM). One limb was trained with unrestricted blood flow whilst in the other limb blood flow was restricted with the use of a pressure applied cuff above the knee (110 mmHg). Regardless of the training load, peak PO(bf), measured using venous occlusion plethysmography increased when LLRT was performed with blood flow restriction compared to no change following LLRT with unrestricted blood flow. A significant increase (P < 0.05) in the area under the blood time-flow curve was also observed following LLRT with blood flow restriction when compared LLRT with unrestricted blood flow. No changes were observed in R (bf) between groups following training. Maximal dynamic strength (1 RM), maximal voluntary contraction and isokinetic strength at 0.52 and 1.05 rad s(-1) also increased (P < 0.05) by a greater extent following resistance training with blood flow restriction. Moreover, 1 RM increased to a greater extent following training at 50% 1 RM compared to 25% 1 RM. These results suggest that 4 weeks LLRT with blood flow restriction provides a greater stimulus to increase peak PO(bf) as well as strength parameters than LLRT with unrestricted blood flow. [ABSTRACT FROM AUTHOR]

This study investigated the energy system contributions of rowers in three different conditions: rowing on an ergometer without and with the slide and rowing in the water. For this purpose, eight rowers were submitted to 2,000 m race simulations in each of the situations defined above. The fractions of the aerobic (W (AER)), anaerobic alactic (W (PCR)) and anaerobic lactic (W ([La-])) systems were calculated based on the oxygen uptake, the fast component of excess post-exercise oxygen uptake and changes in net blood lactate, respectively. In the water, the metabolic work was significantly higher [(851 (82) kJ] than during both ergometer [674 (60) kJ] and ergometer with slide [663 (65) kJ] (P < or = 0.05). The time in the water [515 (11) s] was higher (P < 0.001) than in the ergometers with [398 (10) s] and without the slide [402 (15) s], resulting in no difference when relative energy expenditure was considered: in the water [99 (9) kJ min(-1)], ergometer without the slide [99.6 (9) kJ min(-1)] and ergometer with the slide [100.2 (9.6) kJ min(-1)]. The respective contributions of the W (AER), W (PCR) and W ([La-]) systems were water = 87 (2), 7 (2) and 6 (2)%, ergometer = 84 (2), 7 (2) and 9 (2)%, and ergometer with the slide = 84 (2), 7 (2) and 9 (1)%. VO2, HR and lactate were not different among conditions. These results seem to indicate that the ergometer braking system simulates conditions of a bigger and faster boat and not a single scull. Probably, a 2,500 m test should be used to properly simulate in the water single-scull race. [ABSTRACT FROM AUTHOR]

This study investigated the effect of repeated bouts of short-term, high-intensity cycling exercise on the salivary cortisol, testosterone and immunoglobulin (A) concentrations of 15-16 year old boys. Seventeen apparently healthy schoolchildren (aged 15.5 +/- 0.4 years) participated in this study. All participants completed 6 x 8 s sprints, interspersed with 30 s recovery intervals on a cycle ergometer. Using the passive drool method, salivary samples were taken before, and 5 min after, exercise. The group mean for peak power output was 723.1 +/- 180.3 s. There were significant changes (p < or = 0.05) in both SalT and SalC, 5 min after completing 6 x 8 s cycle sprints. No significant differences (p > 0.05) were recorded for SIg(A). The increases in SalT and SalC reported in this study confirm that repeated bouts of short-term, high-intensity exercise produces significant physiological hormonal responses in adolescent boys, but does not affect mucosal immune function. [ABSTRACT FROM AUTHOR]

The aim was to test the hypothesis that one night of sleep deprivation will impair pre-loaded 30 min endurance performance and alter the cardio-respiratory, thermoregulatory and perceptual responses to exercise. Eleven males completed two randomised trials separated by 7 days: once after normal sleep (496 (18) min: CON) and once following 30 h without sleep (SDEP). After 30 h participants performed a 30 min pre-load at 60% [VO(2 max) followed by a 30 min self-paced treadmill distance test. Speed, RPE, core temperature (T(re)), mean skin temperature (T(sk)), heart rate (HR) and respiratory parameters VO(2 max), VCO(2), VE, RER pre-load only) were measured. Less distance (P = 0.016, d = 0.23) was covered in the distance test after SDEP (6037 (759) 95%CI 5527 to 6547 m) compared with CON (6224 (818) 95%CI 5674 to 6773 m). SDEP did not significantly alter T(re) at rest or thermoregulatory responses during the pre-load including heat storage (0.8 degrees C) and T(sk). With the exception of raised VO(2) at 30 min on the pre-load, cardio-respiratory parameters, RPE and speed were not different between trials during the pre-load or distance test (distance test mean HR, CON 174 (12), SDEP 170 (13) beats min(-1): mean RPE, CON 14.8 (2.7), SDEP 14.9 (2.6)). In conclusion, one night of sleep deprivation decreased endurance performance with limited effect on pacing, cardio-respiratory or thermoregulatory function. Despite running less distance after sleep deprivation compared with control, participants' perception of effort was similar indicating that altered perception of effort may account for decreased endurance performance after a night without sleep. [ABSTRACT FROM AUTHOR]

A reduction in LDL cholesterol and an increase in HDL cholesterol levels are clinically relevant parameters for the treatment of dyslipidaemia, and exercise is often recommended as an intervention. This study aimed to examine the effects of acute, high-intensity exercise ( approximately 90% VO(2max)) and varying carbohydrate levels (control, low and high) on the blood lipid profile. Six male subjects were distributed randomly into exercise groups, based on the carbohydrate diets (control, low and high) to which the subjects were restricted before each exercise session. The lipid profile (triglycerides, VLDL, HDL cholesterol, LDL cholesterol and total cholesterol) was determined at rest, and immediately and 1 h after exercise bouts. There were no changes in the time exhaustion (8.00 +/- 1.83; 7.82 +/- 2.66; and 9.09 +/- 3.51 min) and energy expenditure (496.0 +/- 224.8; 411.5 +/- 223.1; and 592.1 +/- 369.9 kJ) parameters with the three varying carbohydrate intake (control, low and high). Glucose and insulin levels did not show time-dependent changes under the different conditions (P > 0.05). Total cholesterol and LDL cholesterol were reduced after the exhaustion and 1 h recovery periods when compared with rest periods only in the control carbohydrate intake group (P < 0.05), although this relation failed when the diet was manipulated. These results indicate that acute, high-intensity exercise with low energy expenditure induces changes in the cholesterol profile, and that influences of carbohydrate level corresponding to these modifications fail when carbohydrate (low and high) intake is manipulated. [ABSTRACT FROM AUTHOR]

The present study was undertaken to evaluate the acute effect of prior resistance training of varying intensities on energy expenditure and substrate utilization during subsequent aerobic exercise. Eleven males and 21 females completed three experimental trials consisting of (1) aerobic exercise only (C), (2) aerobic exercise preceded by a high-intensity resistance training (HI), and (3) aerobic exercise preceded by a low-intensity resistance training (LO). Resistance training produced an equal volume between HI and LO and consisted of six exercises with each performed for three sets of eight repetitions at 90% of 8-RM in HI and three sets of 12 repetitions at 60% of 8-RM in LO. Aerobic exercise was performed on a cycle ergometer at 50% VO(2)peak for 20 min in all trials. Oxygen uptake (VO2), and carbohydrate and fat oxidation were determined throughout each aerobic exercise session. Fat oxidation rate was higher (P < 0.05) in HI than either LO or C in both males and females. VO2 was also higher (P < 0.05) in HI than either LO or C in females. In males, although between-trial differences in VO2 did not reach statistical significance, they were consistent with the trend seen in females. No differences in carbohydrate oxidation rates were observed across the three trials in either gender group. It appears that in training that combines both aerobic and resistance exercises, performing a comparatively higher intensity resistance exercise first would augment fat utilization and energy expenditure during subsequent aerobic exercise. [ABSTRACT FROM AUTHOR]

The purpose of this study was to investigate whether individual leg cycling could produce higher whole body peak oxygen uptake (VO(2peak)) than two legs cycling during aerobic high intensity interval training in chronic obstructive pulmonary disease (COPD) patients. Nineteen patients trained in 24 supervised cycling sessions either by one leg training (OLT) (n = 12) or by two legs training (TLT) (n = 7) at 4 x 4 min intervals at 85-95% of peak heart rate. Whole body VO(2peak) and peak work rate increased significantly by 12 and 23% in the OLT, and by 6 and 12% in the TLT from pre- to post-training, respectively, and were significantly greater in the OLT than the TLT (P < 0.05). The present study demonstrates that one leg aerobic high intensity interval cycling is superior to two legs in increasing whole body VO(2peak), indicating a muscle rather than a cardiovascular limitation to VO(2peak) in these COPD patients. [ABSTRACT FROM AUTHOR]

The reduced arterial oxygen tension at high altitude impairs the ability to work. Acetazolamide improves arterial oxygen saturation (SaO2) by increasing ventilation but is associated with an increased work and cost of breathing. Depending on the settings, sildenafil can also increases SaO2 possibly through a reduction in pulmonary hypertension and interstitial edema, which could improve ventilation–perfusion matching. The objective of this study is to determine the effects of acetazolamide and sildenafil on ventilatory control and breathing efficiency ( VE/ VCO2) during submaximal steady-state hypoxic exercise in healthy individuals. Following 18 h of hypoxic exposure in an altitude tent at an oxygen concentration of 12.5% (simulated altitude of 4,300 m), 15 participants performed 10 min of hypoxic exercise on a stationary bicycle at 40% of their sea level peak oxygen uptake ( VO2) while randomly receiving sildenafil 40 mg (SIL), acetazolamide 125 mg (ACZ) or a placebo (PLA). There was no difference in VO2 during exercise between conditions while SaO2 was greater with acetazolamide compared to both placebo and sildenafil. Acetazolamide increased ventilation (PLA 49.0 ± 3.2, SIL 47.7 ± 3.1, ACZ 52.1 ± 3.0 l/min) and reduced end-tidal CO2 ( PETCO2) (PLA 32.1 ± 0.8, SIL 32.8 ± 0.9, ACZ 29.2 ± 0.7 mmHg) compared to placebo and sildenafil. Breathing was less efficient with acetazolamide (increased VE/ VCO2) in comparison to placebo and sildenafil (PLA 41.5 ± 1.0, SIL 40.4 ± 1.3, ACZ 45.4 ± 1.0) while sildenafil did not change VE/ VCO2 during hypoxic exercise. In conclusion, acetazolamide increased ventilation and reduced breathing efficiency while sildenafil did not affect breathing efficiency despite a trend toward a blunted ventilatory response, possibly due to a reduction in pulmonary hypertension and/or ventilatory drive, during submaximal hypoxic exercise in healthy individuals. [ABSTRACT FROM AUTHOR]

We investigated the impact of 13 days of "living high-training low" (LHTL) on the antioxidant/prooxidant balance in elite endurance swimmers. Eighteen elite swimmers from the French Swimming Federation were submitted to a 13-day endurance training and divided into two groups: one group trained at 1,200 m and lived in hypoxia (2,500-3,000 m simulated altitude) and the second group trained and lived at 1,200 m. The subjects performed an acute hypoxic test (10 min at 4,800 m) before and 1 day after the training period. Plasma levels of advanced oxidation protein products (AOPP), malondialdehydes (MDA), ferric reducing antioxidant power (FRAP), and lipid-soluble antioxidants were measured before and after the 4,800 m tests. After the training, MDA and AOPP responses to the 4,800 m test were lower than before training for both groups (+10 vs. +2%; P = 0.01 for MDA and +80 vs. +14%; P = 0.01 for AOPP). Thirteen days of LHTL did not modify antioxidant status (FRAP and lipid-soluble antioxidants) despite intakes in vitamins A and E below the recommended daily allowances. The LHTL did not affect the antioxidant status in elite swimmers; however, the normoxic endurance training induced preconditioning mechanisms in response to the 4,800 m test. [ABSTRACT FROM AUTHOR]

Investigations of training effects on exercise energy cost have yielded conflicting results. The purpose of the present study was to compare quadriceps energy cost and oxidative capacity between endurance-trained and sedentary subjects during a heavy dynamic knee extension exercise. We quantified the rates of ATP turnover from oxidative and anaerobic pathways with (31)P-MRS, and we measured simultaneously pulmonary oxygen uptake in order to assess both total ATP production [i.e., energy cost (EC)] and O(2) consumption (O(2) cost) scaled to power output. Seven sedentary (SED) and seven endurance-trained (TRA) subjects performed a dynamic standardized rest-exercise-recovery protocol at an exercise intensity corresponding to 35% of maximal voluntary contraction. We showed that during a dynamic heavy exercise, the O(2) cost and EC were similar in the SED and endurance-trained groups. For a given EC, endurance-trained subjects exhibited a higher relative mitochondrial contribution to ATP production at the muscle level (84 +/- 12% in TRA and 57 +/- 12% in SED; P < 0.01) whereas the anaerobic contribution was reduced (18 +/- 12% in TRA and 44 +/- 11% in SED; P < 0.01). Our results obtained in vivo illustrate that on the one hand the beneficial effects of endurance training are not related to any reduction in EC or O(2) cost and on the other hand that this similar EC was linked to a change regarding the contribution of anaerobic and oxidative processes to energy production, i.e., a greater aerobic energy contribution associated with a concomitant reduction of the anaerobic energy supply. [ABSTRACT FROM AUTHOR]

The aims of this study were to evaluate the effects of both noncircular (ROT) and conventional (CON) chainring systems on aerobic and anaerobic cycling performances of professional cyclists, while analyzing the influence of varying the crank angle of maximum crank arm length of ROT. Fifteen professional road cyclists performed both incremental and sub-maximal aerobic tests and the Wingate anaerobic test in the laboratory. There were no statistical differences between CON and ROT in the aerobic tests, even when the best ROT position (ROT+) was selected. However, in the anaerobic test, maximal (4.2-9.1%) and mean (0.7-4.7%) power outputs were higher in ROT (P < 0.05). These differences were greater when the ROT+ was selected (11.2 and 7.0%, respectively). Our findings suggest that ROT is able to improve anaerobic but not aerobic cycling performance in professional cyclists. Nevertheless, it must be adapted to each cyclist to ensure these improvements. [ABSTRACT FROM AUTHOR]

In a placebo-controlled study, the effects of intermittent hypoxic exposures (IHE) or a placebo control for 10 days, were examined on the extent of exercise-induced hypoxemia (EIH), cerebral and muscle oxygenation (near-infrared spectroscopy) and VO(2peak). Eight athletes who had previously displayed EIH (fall in saturation of arterial oxygen (SaO(2)) of >4% from rest) during an incremental maximal exercise test, volunteered for the present research. Prior to (baseline), and 2 days following (post) the IHE or placebo, an incremental maximal exercise test was performed whilst SaO(2), heart rate, cerebral and muscle oxygenation and respiratory gas exchange were measured continuously. After IHE, but not placebo, EIH was less pronounced at VO(2peak) (IHE group, SaO(2) at VO(2peak) baseline 91.23 +/- 1.10%, post 94.10 +/- 2.19%; P < 0.01, mean +/- SD). This reduction was reflected in an increased ventilation (NS), a lower end-tidal CO(2) (P < 0.01), and lowered cerebral TOI during heavy exercise (90% VO(2peak): -6.1 +/- 6.0 Delta%, P = 0.04). Conversely, muscle tHb at maximal exercise, was increased (2.4 +/- 1.8 DeltamicroM, P = 0.01, mean +/- 95 CL) following IHE, whilst de-oxygenated Hb at 90% of VO(2peak) was reduced (-0.9 +/- 0.8 DeltamicroM, P = 0.02). These data indicate that exposure to IHE can attenuate the degree of EIH. Despite a potential compromise in cerebral oxygenation, exposure to IHE may induce some positive physiological adaptations at the muscle tissue level. We speculate that the unchanged VO(2peak) following IHE might reflect a balance between these central (cerebral) and peripheral (muscle) adaptations. [ABSTRACT FROM AUTHOR]

Hyperthermia may be a consequence of environmental conditions, bacterial or viral infections and/or thyroid storm. This study investigates the acute effect of body temperature elevation on thyroid function and on its scintigraphy studies. Thyroid scintigraphy was performed on New Zealand White rabbits weighing approximately 3-3.5 kg. Each rabbit was injected with 115 MBq (3.1 mCi) technetium-99 m pertechnetate ((99m)Tc pertechnetate). Studies were performed using Gamma camera equipped with a low energy, high resolution, pinhole collimator interfaced with a computer. Static images were acquired 20 min after administration of the radiotracer. Two days later the same protocol was repeated for the same rabbit after increasing the body temperature by 2 degrees C. The experiment was repeated again after a 2-day interval at 3 degrees C, and then after another 2-day interval at 4 degrees C. Plasma free thyroxine (FT(4)), free triiodothyronine (FT(3)) and thyroid stimulating hormone (TSH) were measured at control and at different hyperthermic temperatures (+2, 3, 4 degrees C). We recorded isometric tension of rabbit thyroid artery strips in organ baths during stepwise temperature elevation. During hyperthermia the decrease in thyroid function and thyroid scintigraphy studies was proportional to body temperature elevation. The recording of isometric tension in rabbit thyroid artery strips in organ baths showed vasoconstriction during hyperthermia which is proportional to the heating temperature. Plasma FT(4) and FT(3) level were decreased while TSH levels were not affected by acute fever. Our results indicate that hyperthermia causes a transient decrease in thyroid gland function and scintigraphic patterns on radionuclide studies. Thus, body temperatures must be measured before radionuclide studies in order to ensure that interpretation of data is not influenced by hyperthermia. [ABSTRACT FROM AUTHOR]

The purpose of this study was to investigate the muscular efficiency during cycling exercise under certain total power output (Ptot) or external power output (Pext) experimental conditions that required a large range of pedal rates from 40 to 120 rpm. Muscular efficiency estimated as a ratio of Ptot, which is sum of internal power output (Pint) and Pext, to rate of energy expenditure above a resting level was investigated in two experiments that featured different conditions on a cycle ergometer, which were carried out at the same levels of Ptot (Exp. 1) and Pext (Exp. 2). Each experiment consisted of three exercise tests with three levels of pedal rates (40, 80 and 120 rpm) lasting for 2-3 min of unloaded cycling followed by 4-5 min of loaded cycling. VO2 during unloaded cycling (approximately 430 ml min(-1) for 40 rpm, approximately 640 ml min(-1) for 80 rpm, approximately 1,600 ml min(-1) for 120 rpm) and the Pint (approximately 3 W for 40 rpm, approximately 25 W for 80 rpm, approximately 90 W for 120 rpm) in the two experiments were markedly increased with increasing pedal rates. The highest muscular efficiency was found at 80 rpm in the two experiments, whereas a remarkable reduction (19%) in muscular efficiency obtained at 120 rpm could be attributable to greater O2 cost due to higher levels of Pint accompanying the increased pedal rates. We concluded that muscular efficiency could be affected by the differences in O2 cost and Pint during cycling under the large range of pedal rates employed in this study. [ABSTRACT FROM AUTHOR]

Evidence indicates that repeated-bouts of eccentric exercise (EE) do not exacerbate the extent of muscle damage indices, as compared to a single-bout. We hypothesized that molecular adaptations, under repeated-bouts of EE, would include suppression of muscle repair inhibitory factors such as myostatin and up-regulation of muscle repair positive regulatory factors such as myogenic regulatory factors (MRFs). Fifteen males were recruited for this study. The exercise group (n=9) successfully completed six sets of 15 reps of maximum voluntary eccentric contractions, for six consecutive days, using a dynamometer (Multicont-II). Blood and muscle biopsy samples were obtained from each subject 1 week prior to exercise, 2 days post the first training session, and 24 h after the last training session. Gene expression levels were determined using real-time RT-PCR. Blood samples were analyzed for creatine kinase (CK) and lactate-dehydrogenase (LDH) activity. Repeated-bouts of EE induced a large down-regulation of myostatin mRNA (-73%) which persisted throughout the study. The responses of MRFs were mild. At day 3 only myogenin increased significantly (1.9 fold) while MyoD decreased by 45%. Surprisingly, at day 7, despite the presence of muscle damage indices, all MRFs returned to the pre-exercise levels. The results of the present study showed that repeated-bouts of EE, for six consecutive days, dramatically decreased Myostatin mRNA expression but impaired the expression patterns of MRFs such that, with the exception of myogenin that showed a moderate non-sustained increase, MyoD and MYf5 response was minimal. [ABSTRACT FROM AUTHOR]

This review discusses brain temperature as a physiological parameter, which is determined primarily by neural metabolism, regulated by cerebral blood flow, and affected by various environmental factors and drugs. First, we consider normal fluctuations in brain temperature that are induced by salient environmental stimuli and occur during motivated behavior at stable normothermic conditions. Second, we analyze changes in brain temperature induced by various drugs that affect brain and body metabolism and heat dissipation. Third, we consider how these physiological and drug-induced changes in brain temperature are modulated by environmental conditions that diminish heat dissipation. Our focus is psychomotor stimulant drugs and brain hyperthermia as a factor inducing or potentiating neurotoxicity. Finally, we discuss how brain temperature is regulated, what changes in brain temperature reflect, and how these changes may affect neural functions under normal and pathological conditions. Although most discussed data were obtained in animals and several important aspects of brain temperature regulation in humans remain unknown, our focus is on the relevance of these data for human physiology and pathology. [ABSTRACT FROM AUTHOR]

This investigation was undertaken to examine the influence of intensity fluctuation on metabolic responses during and after exercise. Twenty-four males and 24 females were randomly assigned into one of the four groups consisting of 12 subjects of equal gender. Each group performed one of four 30-min exercise protocols: (1) cycling at a constant power output of 75 W (P1), (2) cycling with power output alternating between 50 and 100 W every 5 min (P2), (3) same as P2 except power output was alternated in a reverse order (P3), and (4) same as P2 except power output was alternated between 25 and 125 W (P4). Each exercise session was followed by a 25-min recovery and all protocols yielded the same mechanical work. Oxygen uptake ( VO2), heart rate (HR), respiratory exchange ratio (RER), and plasma lactate concentrations ([La]) were measured at rest and during exercise and recovery. Ratings of perceived exertion (RPE) were recorded during exercise only. During exercise, VO2, HR and RPE did not differ across the four protocols. RER was higher ( P < 0.05) in P4 than P1 and P2. [La] was higher ( P < 0.05) in P4 than P1 and P3. During recovery, VO2 were lower ( P < 0.05) in P1 than P2, P3, and P4, while [La] was higher in P4 than P3. When the total workload was equated, intensity fluctuation exerted no added effect upon metabolic responses during exercise, but provoked greater energy expenditure following exercise. Reversing the order or increasing the magnitude of intensity fluctuation would not further alter metabolic consequences. [ABSTRACT FROM AUTHOR]

The purpose of this study was to evaluate the methodology of Margaria staircase test (MT) for assessment of muscle anaerobic power. Specifically, we hypothesized that due to the scaling effects the outcome of MT calculated using the standard formula that suggests the power output to be proportional to body mass [P = (m . g . h)/T; P = power output, m = body mass, g = 9.81 m/s(2), h = height of stairs climbed, T = running time] overestimates the effect of body size on the calculated muscle power output. Young and physically active subjects (N = 111) were tested and the relationship between P and body size (S; either body mass or height) was assessed by standard allometric model (i.e., P = a . S ( b ); where a and b were the constant multiplier and allometric parameter, respectively). The results supported the hypothesized relationship by revealing b = 0.66 (95%CI = 0.49-0.83; r = 0.59; P < 0.001) and b = 1.13 (95%CI = 0.49-0.83; r = 0.34; P < 0.001) for body mass and body height, respectively. The obtained values of b (i.e., b < 1 for body mass and b < 3 for body height) suggest that the standard formula of MT overestimates the power output of larger subjects and underestimates the power of smaller ones. Since the results were mainly in line with both the experimental findings and theoretical predictions regarding the general effects of scale on human muscle power, we propose a modified formula that provides normalized power: P ( n ) = P/m (0.67) = [(m . g . h)/T]/m (0.67) = (m (0.33) . g . h)/T. The modified formula could provide a body size independent index of muscle power that would both allow for establishing standards and enable comparison of individuals and groups of individuals of different body dimensions. [ABSTRACT FROM AUTHOR]

The objectives of this study were to (1) continuously assess oxygen uptake and heart rate; (2) quantify the extent to which maximal whole-body cardiorespiratory capacity is utilized during climbing on four routes with the same difficulty but different steepness and/or displacement. Fifteen expert climbers underwent a maximal graded exercise test (MT), on a treadmill, in order to assess their maximal physiological capacity. After MT, four sport routes, equal in difficulty rating but different in steepness and/or displacement, were climbed. Oxygen uptake and heart rate were continuously measured. Respiratory exchange ratio (RER) was calculated. Blood lactate concentration and rating of perceived exertion (RPE) were taken before and directly after climbing. Data were expressed as peak values (HRpeak, VO2peak and RERpeak) and as averages over the entire climb (HRavg, VO2avg and RERavg). During climbing, higher HRpeak and HRavg were found in routes with a vertical upward displacement in comparison to traversing routes with a horizontal displacement. The average absolute and relative oxygen uptake was significantly lower in the traversing route in comparison with the three other routes. The traverse is done at a lower percent of the running maximum. Comparing four routes with the same difficulty but different steepness and/or displacement shows that (1) routes with an upward displacement causes the highest peak and average heart rate; (2) routes with a vertical displacement on overhanging wall is physiologically the most demanding; (3) the traverse is physiologically the less demanding. [ABSTRACT FROM AUTHOR]

In older healthy men, aerobic exercise capacity is related to postischemic flow-mediated dilation of the brachial artery (FMD), but corresponding data in a younger population is not available. In addition, whether submaximal aerobic exercise performance also correlates with this kind of vasomotor reactivity is not known. Therefore, in 15 nonsmoking young healthy men [age 27 (5) years; body mass index: 24 (2) kg/m(2); mean (SD)] with different levels of ordinary physical activity, but not performing upper-extremity training, we measured FMD at 1 min after reactive hyperemia, and pulmonary oxygen uptake (VO(2)) at ventilatory anaerobic threshold (VO(2)AT) and at peak effort (peak VO(2)) during an incremental exercise on a treadmill. In our participants, FMD was 9.1 (3.4)%, VO(2)AT was 40.72 (5.92) ml/kg per min, and peak VO(2) was 52.95 (8.13) ml/kg per min. Using bivariate Pearson's correlation, and in separate multivariate regression analyses, VO(2)AT and peak VO(2) showed a significant and reasonably good correlation with FMD (r = 0.84, P < 0.001 and r = 0.77, P = 0.001, respectively), independent of age, body mass index and serum total cholesterol (beta = 0.77, P < 0.001, R(2) of the overall model = 0.79 and beta = 0.70, P < 0.005, R(2) of the overall model = 0.69, respectively). Our data provide evidence suggesting that in young healthy men a higher submaximal and maximal aerobic exercise performance is associated with a greater FMD of peripheral conduit arteries. [ABSTRACT FROM AUTHOR]

The chemoreflex system controlling ventilation consists of two subsystems, i.e., the central controller (controlling element), and peripheral plant (controlled element). We developed an integral framework to quantitatively characterize individual ventilatory regulation by experimental determination of an equilibrium diagram using a modified metabolic hyperbola and the CO2 response curve. In 13 healthy males, the steady-state arterial CO2 pressure (PaCO2) and minute ventilation (V? e) were measured. To characterize the central controller, we changed fraction of inspired CO2(0, 3.5, 5 and 6% CO2 in 80% oxygen with nitrogen balance) and measured thePaCO2-V? e relation. To characterize the peripheral plant, we alteredV? e by hyper- or hypoventilation using a visual feedback method, which made it possible to control both tidal volume and breathing frequency, and measured theV? e-PaCO2 relation. The intersection between the two relationship lines gives the operating point. The relationship betweenPaCO2 andV? e for the central controller was reasonably linear in each subject (r2=0.808~0.995). The peripheral plant approximated a modified metabolic hyperbolic curve (r2=0.962~0.996). The operating points of the system estimated from the two relationship lines were in good agreement with those measured under the closed-loop condition. The gain of the central controller was 1.9 (1.0) l min-1 mmHg-1 and that of the peripheral plant was 3.0 (0.5) mmHg l-1 min-1. The total loop gain, the product of the two gains, was 5.3 (2.5). We conclude that human ventilatory regulation by the respiratory chemoreflex system can be quantitatively characterized using an equilibrium diagram. This framework should be useful for understanding the mechanisms responsible for abnormal ventilation under various pathophysiological conditions. [ABSTRACT FROM AUTHOR]

It has been well demonstrated that the principal factor responsible for oxidative damage during exercise is the increase in oxygen consumption. However, other theoretical factors (acidosis, catecholamine autoxidation, ischemia-reperfusion syndrome, etc.) that are known to induce, in vitro, oxidative damage may also be operative during short-term supramaximal anaerobic exercise. Therefore, we hypothesized that short-term supramaximal anaerobic exercise (30-s Wingate test) could induce an oxidative stress. Lipid peroxidation markers [serum lipid radical production detected by electron spin resonance (ESR) spectroscopy and plasma malondialdehyde (MDA) levels detected by the thiobarbituric acid reactive substances (TBARS) method], as well as erythrocyte antioxidant enzyme activities [glutathione peroxidase (GPx), superoxide dismutase (SOD)] and erythrocyte glutathione (GSH) levels, were measured at rest, after the Wingate test and during the 40 min of recovery. The recovery of exercise was associated with a significant increase (x2.7) in lipid radical production detected by ESR spectroscopy, as well as with changes in the erythrocyte GSH level (-13.6%) and SOD activity (-11.7%). The paradoxical decrease in plasma TBARS (-23.7%) which was correlated with the peak power developed during the Wingate test ( r=-0.7), strongly suggests that such exercise stimulates the elimination of MDA. In conclusion, this study demonstrates that short-term supramaximal anaerobic exercise induces an oxidative stress and that the plasma TBARS level is not a suitable marker during this type of exercise. [ABSTRACT FROM AUTHOR]

Skeletal muscle adaptations (fiber-type composition, cross-sectional area, myosin heavy chain (MHC) content, and capillarity) were assessed in the vastus lateralis muscle of young men and women after 8 weeks of training with the Sportcord, an elastic resistance device. Ten men [mean (SD) age 20 (1.1) years] and 13 women [20 (1.2) years] performed two sets each to failure of single leg squats and leg extensions at ≅50 repetitions·min–1. Biopsy samples were taken from the right vastus lateralis muscle before and after training. Six fiber types (I, IC, IIC, IIA, IIAB, and IIB) were classified using myofibrillar ATPase histochemistry. Training with the Sportcord caused a small, but significant, increase in one-repetition maximum using free weights and a large increase in repetitions to failure. In addition, elastic resistance training caused an increase in the percentage of fibers classified as type IIAB for both men and women, and a decrease in the percentage of type IIB fibers in the men. MHC analysis supported these findings (a significant increase in the percentage of MHCIIa for the men). The cross-sectional areas of both the type I and IIAB+IIB fibers increased after training for the men, whereas no area changes were found for the women. The capillary:fiber ratio and capillary contacts per fiber type increased significantly for the men, and similar trends were noted for the women. Capillary density did not change in either the men or the women. These data suggest minor changes in fiber type composition (IIB→IIAB), fiber size, and capillarization following short-term training with elastic resistance. Although muscular changes did occur using the Sportcord, the extent of these changes was less than those reported previously for short-term resistance-training programs using free weights. [ABSTRACT FROM AUTHOR]

Oxygen consumption (VO2), ventilation (VE) and heart rate (HR) were studied in five recreational riders with a portable oxygen analyser (K2 Cosmed, Rome) telemetric system, during two different experimental riding sessions. The first one was a dressage session in which the rider successively rode four different horses at a walk, trot and canter. The second one was a jumping training session. Each rider rode two horses, one known and one unknown. The physiological parameters were measured during warm up at a canter in suspension and when jumping an isolated obstacle at a trot and canter. This session was concluded by a jumping course with 12 obstacles. The data show a progressive increase in VO2 during the dressage session from a mean value of 0.70 (0.18) l · min-1 [mean (SD)] at a walk, to 1.47 (0.28) l · min-1 at a trot, and 1.9 (0.3) l · min-1 at a canter. During the jumping session, rider VO2 was 2 (0.33) l · min-1 with a mean HR of 155 beats · min-1 during canter in suspension, obstacle trot and obstacle canter. The jumping course significantly enhanced VO2 and HR up to mean values of 2.40 (0.35) l · min-1 and 176 beats · min-1, respectively. The comparison among horses and riders during the dressage session shows differences in energy expenditure according to the horse for the same rider and between riders. During the jumping session, there was no statistical difference between riders riding known and unknown horses. In conclusion these data confirm that riding induces a significant increase in energy expenditure. During jumping, a mean value of 75% VO2max was reached. Therefore, a good aerobic capacity seems to be a factor determining riding performance in competitions. Regular riding practice and additional physical training are recommended to enhance the physical fitness of competitive riders. [ABSTRACT FROM AUTHOR]

The relationships between cardiac dimensions and physical activity and submaximal working capacity were examined in 198 boys and 154 girls, aged 9–18 years, who were participants in the first phase of the Québec Family Study. The sample was divided into three age groups, 9–12 years, 13–15 years, and 16–18 years. Indicators of physical activity included estimated daily energy expenditure (EE) and time spent in moderate-to-vigorous physical activity (median metabolic equivalents of energy expenditure above resting metabolic rate ≥4.8). Submaximal physical working capacity (PWC150) was determined using a submaximal exercise test on a Monark cycle ergometer. Echocardiographically determined dimensions included posterior wall thickness, septal wall thickness, and left ventricular mass (LVM). The analyses were based on partial correlation and analysis of covariance, controlling for age and body surface area. Relationships between EE/physical activity variables and cardiac dimensions were low and, at best, moderate (r < 0.45). With subjects grouped into tertiles by indicators of physical activity, LVM was significantly different only among 16- to 18-year-old girls (157 g vs 134 g in the highest and lowest quartiles, respectively; P < 0.05). Correlations between cardiac dimensions and PWC150 were also low (r < 0.30), with few significant relationships. In general, cardiac dimensions were not related to habitual physical activity and PWC150 in young subjects aged 9–18 years. However, significant correlations were positive, as expected. LVM may be related to submaximal power output in boys since it accounts for 3% of the variance, after adjusting for age and BSA. [ABSTRACT FROM AUTHOR]

Drug metabolizing capacity is generally reduced in the elderly, and physical exercise has been reported to increase drug oxidative metabolism. The purpose of this investigation was to study the effects of engagement in a program of regular physical exercise on the clearance and metabolite excretion of antipyrine, a marker of oxidative metabolism, in elderly subjects. The saliva clearance of antipyrine and the production clearances of antipyrine metabolites were studied in 37 elderly women (mean age 66 years). Subjects attended 60-min sessions three times a week for 12 weeks. Each session consisted of both aerobic (training of cardiorespiratory capacity) and nonaerobic (training of muscular strength/endurance and flexibility/coordination) exercises performed at 50–75% of maximum oxygen uptake. Antipyrine was administered orally and pharmacokinetic parameters were obtained from saliva and urine samples. After 3 months of participation in the exercise program, salivary antipyrine clearance was significantly increased by 17% mean (SEM) 0.42 (0.02) vs 0.36 (0.02) ml/min/kg; P < 0.05) and the half-life of antipyrine was significantly reduced by 18% (17.9 (1.1) vs 22.3 (1.3) h; P < 0.05). No significant change with exercise was observed in the renal clearance of antipyrine or in the norantipyrine formation clearance, but significant increases were found for hydroxymethylantipyrine [42 (5) vs 32 (4) μl/kg/min; P < 0.05; +31%] and 4-hydroxyantipyrine [243 (18) vs 194 (17) μl/kg/min; P < 0.05; +25%] formation clearances. These findings indicate that regular exercise leads to increased disposition of antipyrine in the elderly and that the main metabolic pathways of the compound are changed differentially. [ABSTRACT FROM AUTHOR]

The aim of this study was to examine whether the increase in the energy cost of running (Cr), previously reported to occur at the end of a prolonged run, could be influenced by the ingestion of either an artificially sweetened placebo (Pl) or a 5.5% carbohydrate (CHO) solution. Ten well-trained triathletes completed three testing sessions within a 3-week period. The aim of the first session was to determine maximal oxygen uptake (VO2max) and the velocity associated with ventilatory threshold (νVT). The second and the third sessions were composed of two submaximal treadmill runs (20 min long, 0% grade, performed at νVT), before and after an 80-min overground run, also conducted at νVT. During these submaximal tests, the subjects ingested (in a random order) either a Pl or CHO solution prior to the first submaximal run and every 20 min after that. During the first session, ventilatory threshold (VT) occurred at [mean (SD)] 81.2 (2.5)% VO2max and 16.5 (0.6) km · h-1. A significant effect of exercise duration was found on Cr (ΔCr) at the end of the run, whatever the solution ingested (ΔCr= 5.7% and 7.01% for CHO and Pl, respectively). A reduction in the respiratory exchange ratio (from 0.98 to 0.90) was observed only at the end of the Pl trial. In this study, Cr seems to be affected only to a minor extent by substrate turnover. Moreover, the increase in the demand for oxygen, estimated from the increase in ventilation, accounted for only a minor proportion of the increase in Cr (11% and 17% for CHO and Pl, respectively). No correlation was found between the changes in Cr and the changes in the other physiological parameters recorded. These results suggest, indirectly, that Cr increases during a 2-h run at 80% VO2max in well-trained subjects can be explained mainly by alterations in neuromuscular performance, which lead to a decrease in muscle efficiency. [ABSTRACT FROM AUTHOR]