1. Directly molecular identification of Brucella melitensis in previously aborted Ewes by Amniocentesis.
- Author
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Abdulla, Alaa Kamil, Daham, Abbas Fadhil, Ulaiwi Al-Karagoly, Hassan Khalaf, and Jassim Al-Delemi, Dhia Hussein
- Subjects
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BRUCELLA melitensis , *AMNIOCENTESIS , *AMNIOTIC liquid , *ENZYME-linked immunosorbent assay , *POLYMERASE chain reaction - Abstract
The main purpose of this study was to detect B. melitensis in amniotic fluids of the pregnant ewes by ELISA and real time PCR, the investigated animals were divided into two groups, group A (n=36) have not abortion history and group B (n= 32) have a previous abortion history. The results of serum samples showed a positive reaction to ELISA for B. melitensis in both groups (A&B) were 19.45%, 37.5%, respectively, while in fetal fluid samples of qPCR result was 30.56% in group A and 56.25% in group B. In conclusion, the real time PCR technique can be successfully used for early diagnosis of B. melitensis by amniocentesis in both known previously aborted ewes as well as the unknown once. [ABSTRACT FROM AUTHOR]
- Published
- 2020