1. [Untitled]
- Author
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Le Thi Muoi, Le Tran Binh, and Dinh Thi Phong
- Subjects
Oryza sativa ,fungi ,food and beverages ,Plant Science ,Horticulture ,Biology ,Somaclonal variation ,RAPD ,Genetic distance ,Genetic marker ,Callus ,Botany ,Genetics ,Genetic variability ,Desiccation ,Agronomy and Crop Science - Abstract
Genetic modification from selfed progenies of 18 rice (Oryza sativa L.) plants regenerated from callus tissues which survived desiccation, were investigated at the DNA level using the random amplified polymorphic DNA (RAPD) method. Twelve 10-mer random primers were used to amplify DNA of progenies from the regenerated plants, and a total of 228 PCR products and 1780 DNA fragments were obtained by primers, generating between four to thirteen major bands. The size of the amplified fragments ranged from 0.2 to 2.55 kb. The results showed that 10 out of 12 primers produced polymorphic bands, two primers (RA31 and RA185) showed no polymorphism among plants tested. A dendrogram of the genetic distance was constructed based on their polymorphism, demonstrating that somaclonal variation exists in rice plants regenerated from callus which survived the desiccation treatment. Part of this variation can be useful in rice breeding.
- Published
- 2001
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