1. The Sg-6 saponins, new components in wild soybean ( Glycine soja Sieb. and Zucc.): polymorphism, geographical distribution and inheritance.
- Author
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Krishnamurthy, Panneerselvam, Tsukamoto, Chigen, Singh, Ram, Lee, Jeong-Dong, Kim, Hong-Sig, Yang, Seung-Hwan, and Chung, Gyuhwa
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PHYTOGEOGRAPHY , *SAPONINS , *SOYBEAN , *GENETIC polymorphisms in plants , *CHEMICAL composition of plants , *THIN layer chromatography , *GLYCOSIDES - Abstract
Hypocotyl saponin composition of 1,198 accessions of wild soybean ( Glycine soja) collected from China, Korea, Japan and Russia Far East was analyzed by thin-layer chromatography to determine polymorphic variation and geographical distribution. Eight common distinguishable saponin phenotypes were identified: Aa, Ab, AaBc, AbBc, Aa+α, Ab+α, AaBc+α and AbBc+α. The latter four +α type were new. All eight types were identified in China. Type Ab+α was absent in Korea. Types Ab+α and AbBc+α, and Aa+α and Ab+α were not identified in Japan and Russia far east, respectively. Six new triterpene saponins were detected in +α type via LC-PDA/MS/MS analyses. They were, tentatively, designated as H-αg, H-αa, I-αg, I-αa, J-αg and J-αa. These saponins were inherited together by a single dominant allele. A gene symbol Sg- 6 was assigned. Hence, the new saponins were collectively named as Sg-6 saponins. The frequency of Sg- 6 allele was 17.6 % in Chinese, 10.0 % in Korean and 1.0 % in Japanese wild soybean. The wild soybeans having Sg-6 saponins can be utilized in soybean breeding programs as well as in saponin biosynthesis studies in soybean. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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