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1. The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii.

Author

Remacle C, Cline S, Boutaffala L, Gabilly S, Larosa V, Barbieri MR, Coosemans N, and Hamel PP

Subjects
Algal Proteins genetics, Amino Acid Sequence, Animals, Arginine metabolism, Chlamydomonas reinhardtii genetics, Chlamydomonas reinhardtii metabolism, Molecular Sequence Data, Ornithine-Oxo-Acid Transaminase genetics, Plastids genetics, Plastids metabolism, Protein Transport, Algal Proteins metabolism, Chlamydomonas reinhardtii enzymology, Ornithine-Oxo-Acid Transaminase metabolism, Plastids enzymology
Abstract

Here we report the characterization of the Chlamydomonas reinhardtii gene ARG9, encoding the plastid resident N-acetyl ornithine aminotransferase, which is involved in arginine synthesis. Integration of an engineered ARG9 cassette in the plastid chromosome of the nuclear arg9 mutant restores arginine prototrophy. This suggests that ARG9 could be used as a new selectable marker for plastid transformation.

Published
2009
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