1. High-throughput miRNA sequencing of the human placenta: expression throughout gestation
- Author
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Gonzalez, Tania L, Eisman, Laura E, Joshi, Nikhil V, Flowers, Amy E, Wu, Di, Wang, Yizhou, Santiskulvong, Chintda, Tang, Jie, Buttle, Rae A, Sauro, Erica, Clark, Ekaterina L, DiPentino, Rosemarie, Jefferies, Caroline A, Chan, Jessica L, Lin, Yayu, Zhu, Yazhen, Afshar, Yalda, Tseng, Hsian-Rong, Taylor, Kent, Williams, John, and Pisarska, Margareta D
- Subjects
Genetics ,Human Genome ,Biotechnology ,Reproductive health and childbirth ,Adult ,Biomarkers ,Computational Biology ,Female ,Gene Expression Profiling ,Gene Expression Regulation ,Gestational Age ,High-Throughput Nucleotide Sequencing ,Humans ,Male ,MicroRNAs ,Placenta ,Pregnancy ,Pregnancy Outcome ,Transcriptome ,chorionic villi ,gestational differences ,human transcriptome ,miRNA ,miRNA atlas ,miRNome ,placenta ,pregnancy ,stable miRNAs ,uncomplicated pregnancies ,Clinical Sciences - Abstract
Aim: To understand miRNA changes across gestation in healthy human placentae. This is essential before miRNAs can be used as biomarkers or prognostic indicators during pregnancy. Materials & methods: Using next-generation sequencing, we characterize the normative human placenta miRNome in first (n = 113) and third trimester (n = 47). Results & conclusion: There are 801 miRNAs expressed in both first and third trimester, including 182 with similar expression across gestation (p ≥ 0.05, fold change ≤2) and 180 significantly different (false discovery rate 2). Of placenta-specific miRNA clusters, chromosome 14 miRNA cluster decreases across gestation and chromosome 19 miRNA cluster is overall highly expressed. Chromosome 13 clusters are upregulated in first trimester. This work provides a rich atlas of healthy pregnancies to direct functional studies investigating the epigenetic differences in first and third trimester placentae.
- Published
- 2021