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1. Citrate Synthesis from Fatty Acids and Amino Acids in Rat Ventral Prostate

Author

Toghrol F, Costello Lc, and Franklin Rb

Subjects
Male, Oxaloacetates, Transamination, Palmitates, Fatty Acids, Nonesterified, Biochemistry, chemistry.chemical_compound, Acetyl Coenzyme A, Prostate, Aspartic acid, medicine, Animals, Citrate synthase, Citrates, Amino Acids, Beta oxidation, chemistry.chemical_classification, Aspartic Acid, biology, Acetyl-CoA, Fatty acid, Rats, Amino acid, medicine.anatomical_structure, chemistry, biology.protein, Ketoglutaric Acids
Abstract

The source of the remarkably high citrate level of prostate was investigated in prostate fragments. L-Aspartate and alpha-keto-glutarate formed oxaloacetate through transamination. Neither pyruvate nor acetate was as effective as palmitate in increasing the citrate level. The result suggested that the source of citrate in rat ventral prostate is oxaloacetate from amino acid transamination and acetyl CoA from fatty acid oxidation. Furthermore, the result indicated that carbohydrates contribute little to citrate production in ventral prostate.

Published
1980
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2. Citrate uptake and oxidation by fragments of rat ventral prostate

Author

Franklin Rb, Littleton Gk, and Costello Lc

Subjects
Ventral prostate, Male, medicine.medical_specialty, Kidney, Isocitrates, Chemistry, Malates, Prostate, Biological Transport, In Vitro Techniques, Biochemistry, In vitro, Rats, Kinetics, Endocrinology, medicine.anatomical_structure, Organ Specificity, Internal medicine, medicine, Animals, Citrates, Oxidation-Reduction, Prostatic tissue
Abstract

Citrate oxidation was studied utilizing an in vitro preparation of rat ventral prostate which was very similar, with respect to citrate metabolish, to the intact prostate. The rate of citrate oxidation was very slow in comparison to kidney, although citrate entered prostatic tissue and accumulated intracellularly. Citrate was converted to isocitrate at a rate which resulted in a constant citrate/isocitrate ratio over a 10-fold variation in medium citrate concentration. The prostate oxidized significantly more alpha-ketoglutarate and malate than citrate. These results suggested that limited citrate oxidation could account for the accumulation of high prostatic citrate levels.

Published
1977

3. Aconitase activity, citrate oxidation, and zinc inhibition in rat ventral prostate.

Author

Costello LC and Franklin RB

Subjects
Animals, Citric Acid, Kidney enzymology, Male, Mitochondria enzymology, Oxidation-Reduction drug effects, Rats, Aconitate Hydratase antagonists & inhibitors, Citrates metabolism, Prostate enzymology, Zinc pharmacology
Abstract

Aconitase activity and citrate oxidation were determined in mitochondrial preparations from rat ventral prostate. Aconitase activity in prostate mitochondria was approximately 10% of the activity observed in kidney preparations. The prostate aconitase activity was completely inhibited by the addition of 1.0 mmol/l zinc and kidney aconitase was inhibited by approximately 50%. The presence of 1.0 mmol/l zinc also markedly inhibited the formation of isocitrate from cis-aconitate. The reduction of NADP by citrate was markedly inhibited (approx. 95%) by 1.0 mmol/l zinc as compared with a 70 and 40% inhibition of aconitate--and isocitrate--stimulated reduction, respectively. These results indicate that zinc might be a strong inhibitor of aconitase activity with an inhibitory effect on isocitrate dehydrogenase also. The effect of 1.0 mmol/l zinc on citrate oxidation was studied by determining 14CO2 production from 6-14C-citrate. 14CO2 was significantly inhibited by zinc. These results further corroborate the conclusion that citrate accumulation in prostate might be the result of limited citrate oxidation which results from a combination of low aconitase activity and possibly zinc inhibition.

Published
1981
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4. Net citrate production by isolated prostate epithelial cells.

Author

Costello LC, Akuffo V, and Franklin RB

Subjects
Acetyl Coenzyme A metabolism, Animals, Aspartic Acid metabolism, Citric Acid, Epithelium enzymology, Ketoglutaric Acids metabolism, Male, Mitochondria enzymology, Oxaloacetates metabolism, Pyruvates metabolism, Pyruvic Acid, Rats, Rats, Inbred Strains, Aspartate Aminotransferases metabolism, Citrates biosynthesis, Glutamate Dehydrogenase metabolism, Prostate enzymology
Abstract

The net production of citrate from exogenous substrates by rat ventral prostate was investigated. The preparation of isolated prostate epithelial cells was described. These cells were capable of oxidizing pyruvate (5 mmol/l) as a source of acetyl coenzyme A. The addition of aspartate + alpha-ketoglutarate (5 mmol/l) in the presence of pyruvate resulted in significant net production of citrate and excess oxalacetate. In the presence of aspartate and glutamate, the cells were capable of producing citrate without excessive oxalacetate production. Neither glucose alone nor glucose plus pyruvate resulted in net citrate production. The results demonstrated that aspartate could serve as a 4-carbon source of oxalacetate for citrate synthesis. Furthermore, the results indicate the intramitochondrial operation of a glutamate-aspartate-citrate pathway involving mitochondrial aspartate aminotransferase and glutamic dehydrogenase activities in prostate epithelial cells.

Published
1988
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