1. Degradation of crude oil by mixed cultures of bacteria isolated from the Qinghai-Tibet plateau and comparative analysis of metabolic mechanisms
- Author
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Wei Zhang, Shi-Weng Li, Guangxiu Liu, Binglin Zhang, Gaosen Zhang, Ruiqi Yang, Yong-Na Wu, Xiukun Wu, Tuo Chen, Y.P. Li, and Faegheh Moazeni
- Subjects
Health, Toxicology and Mutagenesis ,010501 environmental sciences ,Tibet ,01 natural sciences ,Bioremediation ,Environmental Chemistry ,Ecotoxicology ,Soil Pollutants ,Food science ,Incubation ,Soil Microbiology ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,biology ,Bacteria ,Chemistry ,General Medicine ,Biodegradation ,Crude oil ,biology.organism_classification ,Pollution ,Hydrocarbon ,Biodegradation, Environmental ,Petroleum ,Degradation (geology) - Abstract
This study investigates the biodegradation of crude oil by a mixed culture of bacteria isolated from the Qinghai-Tibet plateau using gas chromatography-mass spectrometer (GC-MS) and the gravimetric method. The results showed that a mixed culture has a stronger ability to degrade hydrocarbon than pure cultures. Once both Nocardia soli Y48 and Rhodococcus erythropolis YF28-1 (8) were present in a culture, the culture demonstrated the highest crude oil removal efficiency of almost 100% after 10 days of incubation at 20 °C. Moreover, further analysis of the degradation mechanisms used by the above strains, which revealed utilization of different n-alkane substrates, indicated the diversity of evolution and variations in different strains, as well as the importance of multiple metabolic mechanisms for alkane degradation. Therefore, it is concluded that a mixed culture of Y48 and YF28-1 (8) strains can provide a more effective method for bioremediation of hydrocarbon-contaminated soil in permafrost regions.
- Published
- 2018