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Your search keyword '"Laboratoire de Physiologie et Génomique des Poissons ( LPGP )"' showing total 3 results
Start Over Author "Laboratoire de Physiologie et Génomique des Poissons ( LPGP )" Journal environmental science and pollution research
3 results on '"Laboratoire de Physiologie et Génomique des Poissons ( LPGP )"'

1. Long-term disruption of growth, reproduction, and behavior after embryonic exposure of zebrafish to PAH-spiked sediment

Author

Marie-Laure Bégout, Jérémy Potier, Xavier Cousin, Jérôme Cachot, Karyn Le Menach, Laura Lyphout, Hélène Budzinski, Marie Hélène Devier, Caroline Vignet, Laboratoire d'Écotoxicologie, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ressources Halieutiques (LRH), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ANR ConPhyPop (2009-002), CPER A2E, European Regional Development Fund, Region Poitou-Charentes, Ifremer, ANR-09-CESA-0002,CONPHYPOP,Contamination et physiologie des poissons aux polluants(2009), and Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Geologic Sediments, Lifecycle, Embryo, Nonmammalian, animal structures, Offspring, Health, Toxicology and Mutagenesis, media_common.quotation_subject, Polycyclic aromatic hydrocarbon, Biology, Toxicology, chemistry.chemical_compound, polycyclic aromatic hydrocarbon, Animals, Environmental Chemistry, Ecotoxicology, 14. Life underwater, Polycyclic Aromatic Hydrocarbons, Zebrafish, media_common, chemistry.chemical_classification, Larva, Danio rerio, Behavior, Animal, offspring, Reproduction, [SDV.BA]Life Sciences [q-bio]/Animal biology, anxiety-like behavior, fungi, Anxiety-like behavior, Sediment, General Medicine, Phenanthrene, Pollution, lifecycle, chemistry, danio rerio, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Environmental chemistry, Pyrene, Female, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Water Pollutants, Chemical
Abstract

A natural sediment spiked with three individual polycyclic aromatic hydrocarbons (PAHs; pyrene, phenanthrene and benzo[a]pyrene) was used to expose zebrafish embryos and larvae during 4 days. The total PAH concentration was 4.4 μg g(-1) which is in the range found in sediment from contaminated areas. Quantification of metabolites in the larvae after exposure confirmed the actual contamination of the larvae and indicated an active metabolism especially for pyrene and benzo[a]pyrene. After a transfer in a clean medium, the larvae were reared to adulthood and evaluated for survival, growth, reproduction, and behavior. Measured endpoints revealed a late disruption of growth (appearing at 5 months) and a trend toward a lower reproductive ability. Adults of embryos exposed to sediment spiked with PAHs displayed lethargic and/or anxiety-like behaviors. This latter behavior was also identified in offspring at larval stage. All together, these effects could have detrimental consequences on fish performances and contribution to recruitment.

Published
2014
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2. Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish - part I: Survival and growth

Author

José-Luis Zambonino-Infante, Marie Hélène Devier, Laura Frère, Xavier Cousin, David Mazurais, Florane Le Bihanic, Julie Lucas, Karyn Le Menach, Hélène Budzinski, Laura Lyphout, Marie-Laure Bégout, Caroline Vignet, Prescilla Perrichon, Laboratoire d'Écotoxicologie, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ressources Halieutiques (LRH), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ANR ConPhyPop (2009-002), CPER A2E, European Regional Development Fund, Région Poitou-Charentes, Ifremer, Conseil Général de Charente-Maritime, Ministère de l'Enseignement Supérieur de la Recherche, ANR-09-CESA-0002,CONPHYPOP,Contamination et physiologie des poissons aux polluants(2009), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés - UMR 7266 (LIENSs), and Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, Health, Toxicology and Mutagenesis, Polycyclic aromatic hydrocarbon, digestion, 010501 environmental sciences, 01 natural sciences, jaw, Polycyclic Aromatic Hydrocarbons, Zebrafish, chemistry.chemical_classification, 0303 health sciences, Meal, Larva, Light crude oil, biology, Chemistry, Reproduction, [SDV.BA]Life Sciences [q-bio]/Animal biology, General Medicine, Heavy oil, Contamination, Pollution, Petroleum, danio rerio, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Environmental chemistry, Female, Digestion, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Environmental Monitoring, light crude oil, Life cycle, 03 medical and health sciences, polycyclic aromatic hydrocarbon, Animals, Environmental Chemistry, Ecotoxicology, 14. Life underwater, 030304 developmental biology, 0105 earth and related environmental sciences, Danio rerio, alkylated PAH, Alkylated PAH, ACL, biology.organism_classification, Animal Feed, lifecycle, Jaw, heavy oil, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Water Pollutants, Chemical
Abstract

The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6-6.7 μg g(-1) for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel (HO) and a light crude oil (LO). Fish growth was inhibited by all PAH fractions and the effects were sex specific: as determined with 9-month-old adults, exposure to the highest PY inhibited growth of females; exposure to the highest HO and LO inhibited growth of males; also, the highest HO dramatically reduced survival. Morphological analysis indicated a disruption of jaw growth in larvae and malformations in adults. Intestinal and pancreatic enzyme activities were abnormal in 2-month-old exposed fish. These effects may contribute to poor growth. Finally, our results indicate that PAH mixtures of different compositions, representative of situations encountered in the wild, can promote lethal and sublethal effects which are likely to be detrimental for fish recruitment.

Published
2014
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3. Development of a reference artificial sediment for chemical testing adapted to the MELA sediment contact assay

Author

Laure Landi, Prescilla Perrichon, Karyn Le Menach, Jérôme Cachot, Christelle Clérandeau, Xavier Cousin, Hélène Budzinski, Florane Le Bihanic, Université Sciences et Technologies - Bordeaux 1, UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Ecotoxicology Laboratory, Place Gaby Coll, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), ANR ConPhyPop (2009-002), Ministère de l'Enseignement Supérieur et de la Recherche, Conseil Général de Charente-Maritime, Ifremer, and Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Spiked sediment, Geologic Sediments, embryotoxicity, 010504 meteorology & atmospheric sciences, Health, Toxicology and Mutagenesis, Oryzias, Polycyclic aromatic hydrocarbon, 010501 environmental sciences, Artificial sediment, 01 natural sciences, japanese medaka embryos, chemistry.chemical_compound, Teratogenicity, PAHs, spiked sediment, Animals, Environmental Chemistry, Ecotoxicology, artificial sediment, 14. Life underwater, Polycyclic Aromatic Hydrocarbons, 0105 earth and related environmental sciences, chemistry.chemical_classification, Fluoranthene, Pollutant, Japanese medaka embryos, [SDV.BA]Life Sciences [q-bio]/Animal biology, Sediment, Sorption, General Medicine, Contamination, Pollution, chemistry, 13. Climate action, Larva, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Environmental chemistry, Embryotoxicity, Pyrene, Biological Assay, teratogenicity
Abstract

Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo-larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial sediment was tested using a model polycyclic aromatic hydrocarbon (PAH), fluoranthene. The sediment was then validated with two other model PAHs, benz[a]anthracene and benzo[a]pyrene. Various developmental end points were recorded, including survival, embryonic heartbeat, hatching delay, hatching success, larval biometry and abnormalities. The final artificial sediment composition was set at 2.5 % dry weight (dw) Sphagnum peat, 5 % dw kaolin clay and 92.5 % dw silica of 0.2- to 0.5-mm grain size. In contrast with natural sediments, the chemical components of this artificial matrix are fully defined and readily identifiable. It is totally safe for fish embryos and presents relatively high sorption capacities for hydrophobic compounds. Studies with other hydrophobic and metallic contaminants and mixtures should be performed to further validate this artificial sediment.

Published
2014
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