1. Aporrectodea caliginosa, a suitable earthworm species for field based genotoxicity assessment?
- Author
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Anamaria Štambuk, Maja Šrut, Goran Klobučar, Luka Traven, Želimira Cvetković, Martina Merkas, and Ivana Husnjak
- Subjects
Eisenia fetida ,Pentachlorophenol ,Croatia ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,Toxicology ,medicine.disease_cause ,01 natural sciences ,chemistry.chemical_compound ,11. Sustainability ,medicine ,Animals ,Soil Pollutants ,Oligochaeta ,Vehicle Emissions ,0105 earth and related environmental sciences ,biology ,Copper toxicity ,Earthworm ,04 agricultural and veterinary sciences ,General Medicine ,medicine.disease ,biology.organism_classification ,Pollution ,Soil contamination ,Comet assay ,DNA damage ,NRRT assay ,coelomocytes ,Aporrectodea caliginosa ,chemistry ,Neutral Red ,Environmental chemistry ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Biological Assay ,Comet Assay ,Copper ,Genotoxicity ,Cadmium ,Environmental Monitoring - Abstract
There is a growing interest for the application of biomakers to field-collected earthworms. Therefore we have evaluated the usability of native populations of endogeic, widely distributed earthworm Aporrectodea caliginosa in the assessment of soil genotoxicity using the Comet assay. Validation of the Comet assay on earthworm coelomocytes has been established using commercially available Eisenia fetida exposed to copper, cadmium, and pentachlorophenol, along with A. caliginosa exposed to copper in a filter paper contact test. Neutral red retention time (NRRT) assay was conducted on copper exposed and field-collected earthworms. Significant DNA and lysosomal damage was measured using Comet and NRRT assays in native populations of A. caliginosa sampled from the polluted soils in the urban area in comparison to the earthworms from the reference site. The results of this study confirm the employment of A. caliginosa as a suitable species for the in situ soil toxicity and genotoxicity field surveys.
- Published
- 2011
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