1. Mutagenesis assays on urines produced by patients administered adriamycin and cyclophosphamide.
- Author
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Newman MA, Hee SS, and Schoeny RS
- Subjects
- Animals, Cyclophosphamide urine, Doxorubicin urine, Drug Therapy, Combination, Glucuronidase metabolism, Humans, Kidney Neoplasms drug therapy, Liver Neoplasms drug therapy, Male, Microsomes drug effects, Microsomes metabolism, Mutagenicity Tests, Rats, Rats, Inbred Strains, Salmonella drug effects, Sulfatases metabolism, Cyclophosphamide adverse effects, Doxorubicin adverse effects, Mutation
- Abstract
The XAD-2 resin concentration/elution system for concentration of mutagens contained in urines was optimized for cancer patients who had been administered such antineoplastic agents as adriamycin (ADR; doxorubicin), cyclophosphamide (CP), methotrexate, vincristine, and 5-fluorouracil. In the reverse mutation assay, Salmonella typhimurium strains TA1535 and TA98 differentiated between CP (with S9 fraction) and ADR (without S9), respectively. No dose-response for CP was observed. There was a dose-response to ADR by TM677 in the presence of S9 using a forward mutation assay. However, while the reverse mutation assays successfully detected ADR and CP administration in the presence of each other in terms of urine mutagenicity, the forward mutation assay did not, since unidentified CP metabolites were also detected in the latter. None of these systems detected mutagenic urines from tobacco smokers, although reaction of these urines with beta-glucuronidase allowed this type of source to be detected also.
- Published
- 1990
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