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Start Over Author "Yun, Chi" Journal entomological research
13 results on '"Yun, Chi"'

2. Characterization of Tissue-Ferritin Purified from Wax Moth, Galleria mellonella

Author

Park Jong-Bae, Yuk Ji-Eun, Choi Hye-Kyoung, Choi Chi-Won, Han Jikhyon, Yun Chi-Young, Hwang Seock-Yeon, Koh Sang Kyun, and Seo Dong-Hwan

Subjects
Gene isoform, Wax, Malpighian tubule system, animal structures, Protein subunit, fungi, Subunit mrna, Biology, biology.organism_classification, Ferritin, Galleria mellonella, Biochemistry, Insect Science, visual_art, biology.protein, visual_art.visual_art_medium, Integument
Abstract

Tissue-ferritin was homogeneously purified from the hemolymph-free whole body of Galleria mellonella larvae. Tissue-ferritin was composed of four subunits, 26, 30, 32 and 34 kDa. The 34 kDa subunit among them was specifically found in tissue ferritin while its N-terminal sequence of 19 amino acid residues was identical to that of 32 kDa hemolymph-ferritin subunit. It was observed that the 32 and 34 kDa subunit of tissue-ferritin were glycosylated like 32 kDa subunit of hemolymph-ferritin. It suggests that the 34 kDa subunit is an isoform of 32kDa ferritin subunit according post-transcriptional modification. By semi quantitative RT-PCR, the distribution of 32 kDa ferritin subunit mRNA was observed in fat body, Malpighian tubules, integument, and muscle, but found in a very small amount in silk gland or not found in gut. On the other hand, 26 kDa subunit mRNA was found in all organs tested although it was in a small quantity found in gut and silk gland. By dietary Hg treatment, 34 kDa subunit of tissue-ferritin was clearly up-regulated.

Published
2005
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12. Comparison between hemolymph and tissue ferritin in response to heavy metal feeding in the wax moth Galleria mellonella.

Author

Choi, Chi-Won, Seo, Dong-Hwan, Yuk, Ji-Eun, and Yun, Chi-Young

Subjects
GREATER wax moth, HEMOLYMPH, FERRITIN, BLOOD, CARRIER proteins, GALLERIA
Abstract

The effects of seven heavy metals on hemolymph and tissue ferritin in Galleria mellonella were examined by western blotting. Hemolymph ferritin was clearly upregulated by Hg, while tissue ferritin was dramatically upregulated by Cd, Cu, Mn, Zn and Ni. The level of hemolymph ferritin for Hg and Cd increased approximately 1.5-fold over normal controls, while the level of tissue ferritin for Cd, Cu, Mn, Zn and Ni increased more than twofold, indicating that the regulation of hemolymph and tissue ferritin differ with the different heavy metals. Compared to normal control insects not fed CdCl2, ferritin subunit mRNA from the Malpighian tubules, muscle, the gut and the integument was clearly downregulated, as determined by semi-quantitative reverse transcription–polymerase chain reaction, but ferritin subunit mRNA from the silk gland and the fat body did not show any change. These results suggest that in G. mellonella, tissue ferritin is responsible for Cd stress and is thus important for survival of the moth, and that tissue and hemolymph ferritin have different ferritin-inducible mechanisms in response to dietary CdCl2. [ABSTRACT FROM AUTHOR]

Published
2006
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