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Ultraviolet radiation has negative effects on different living organisms. The effects of ultraviolet radiation have been widely studied on some insects, such as stored‐product pests. However, few studies have examined the effects of ultraviolet radiation on mosquitoes. In this study, we report the effect of ultraviolet radiation type C, or ultraviolet C, on the immature stages of Culex quinquefasciatus. Our results suggest that early larval instars were more susceptible to ultraviolet C than late instars, and longer ultraviolet C exposure times were more lethal to larvae regardless of age. Ultraviolet C caused 100% mortality in pupae after 11 minutes of exposure. No egg hatching was observed after 15 minutes of exposure. Mortality in immature stages occurred over time, especially during larval molting and adult emergence. [ABSTRACT FROM AUTHOR]