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Introduction: The reasons for the decrease in blood cultures were investigated and the rate and aetiology of bacteremia and contaminated blood cultures collected from COVID and non-COVID patients were assessed. Methods: We performed a retrospective analysis in a tertiary hospital in Spain during the COVID period from 4th March 2020 to 21thJune 2020. Results: The number of blood cultures processed was 5313, representing 22.7 % and 18.8 % of decrease compared to the same months of 2019 and 2018, respectively (p=0.173). The rate of bacteremia was 1.2% higher among COVID-patients than among non-COVID patients (p

Purpose Gordonia species are known to be opportunistic human pathogens causing secondary infections. We present the second case in the world of endocarditis caused by Gordonia bronchialis and a review of all the cases of endocarditis caused by Gordonia spp. Methods The identification was performed by matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing were performed to confirm the identification. Antimicrobial susceptibility was performed by MIC test Strip on Mueller-Hinton agar supplemented with 5% defibrinated sheep blood according to Clinical and Laboratory Standards Institute. Results Pacemaker-induced endocarditis due to Gordonia bronchialis infection was determined in an 88-year old woman. The patient was treated with ceftriaxone and ciprofloxacin until completing 6 weeks from the pacemaker explant with a good evolution. Conclusion The case presented supports the pathogenic role of Gordonia bronchialis as an opportunistic pathogen and highlights the high risk of suffering infections caused by environmental bacteria.

Introduction No cases of human brucellosis caused by Brucella suis has been reported in Spain. Methods This study involved interviews with the case and his co-workers, inspection of their workplace, checking infection control measures, and typing the Brucella strain isolated in the blood culture. Results Brucella suis biovar 1 strain 1330 was isolated from a patient who worked in a waste treatment plant. Food borne transmission, contact with animals, and risk jobs were ruled out. An accidental inoculation with a contaminated needle from a research laboratory waste container was identified as the most probable mode of transmission. Conclusion There should be controls to ensure that waste containers are sealed.

IntroducciOn El objetivo de nuestro trabajo consiste en revisar el resultado de los cultivos microbiologicos de Bordetella spp. durante un periodo de 19 anos, desde enero de 1984 hasta diciembre de 2002. MEtodos Toma pernasal y siembra inmediata en placas de agar-carbon suplementadas con 10% de sangre de carnero y 40 mg/l de cefalexina. Identificacion mediante pruebas bioquimicas y aglutinacion con antisueros especificos. Recopilacion de los resultados microbiologicos y aspectos epidemiologicos (edad, sexo, paciente ingresado o ambulante, periodo estacional y distribucion anual). Resultados Se cultivaron 2.064 muestras con 269 resultados positivos (13,03%): 244, B. pertussis; 23, B. parapertussis, y 2, B. bronchiseptica. El 36,9% de los casos se dio en menores de 7 meses, el 38,9% en ninos de 7 meses a 6 anos y el 24,3% en mayores de 6 anos. Conclusiones El constante numero de casos a lo largo del periodo de estudio demuestra la circulacion continua de Bordetella spp. en nuestra area de salud. Pese al limitado numero de resultados positivos en adolescentes y adultos seria conveniente considerar la investigacion de tos ferina en estos grupos de edad. El cultivo sigue siendo el metodo diagnostico de referencia y es de especial utilidad en la confirmacion de brotes.

This retrospective study reviews the findings from cultures positive for Bordetella spp. obtained over a 19-year period, from January 1984 to December 2002.Nasopharyngeal swabs were directly plated onto charcoal agar supplemented with 10% sheep blood and cephalexin (40 mg/L). Identification of suspected colonies was done by biochemical testing and slide agglutination with specific antisera for B. pertussis and B. parapertussis. The study includes microbiological findings and epidemiological data (age, sex, yearly distribution, seasonal period and number of inpatients and outpatients).The 2064 nasopharyngeal specimens yielded 269 positive cultures: B. pertussis was isolated from 244 patients, B. parapertussis from 23 and B. bronchiseptica from two. Among the positive patients, 36.9% were younger than 7 months, 38.8% were 7 months to 6 years old and 24.3% were older than 6 years of age.The constant number of cases along the 19 years of the study shows that Bordetella spp. continues to circulate in our area. A high percentage of positive cultures were obtained from the group of children theoretically immunized by vaccination. Despite the low number of cases in young people and adults, adult pertussis seems to be a greater public health threat than was previously suspected and probably deserves to be further investigated. The gold standard for the diagnosis of Bordetella spp. infection is still recovery of the organism by culture, and it is especially useful for confirming outbreaks.

The object of our research is to analyse the microbiological results of the samples which have been obtained by means of fibronchoscopy (FB) from HIV positive patients from 1991 until 1993.Sixty fibrobronchoscopies were carried out on fifty-seven HIV positive patients. In every case, samples of bronchoaspirate (BAS), bronchoalveolar lavage (BAL) and telescoping plugged catheter (TPC) were cultured; the last two in a quantitative way. Pneumocystis carinii was investigated in BAL by means of immunofluorescence with monoclonal antibodies.Some microorganisms were isolated in forty-seven bronchoscopies. Thirteen episodes resulted negative. The most frequent etiologic agent was Pneumocystis carinii (seventeen cases). The etiology of fifteen episodes was polymicrobial. The intersticial radiological pattern was the predominant one. It was observed in twenty-seven cases. With regard to immunity, 91% of the patients showed CD4200.In our research work, the samples that have been obtained by means of FB showed a high percentage of diagnoses; that is the reason why we regard this technique as very useful for the diagnosis of pneumonia in patients with AIDS. Due to the large number of bacterian pneumonia, we consider necessary not only the use of BAL, but also that of TPC in these processes.

sugirieron que podian ser miembros de dos nuevas especies de estreptococos, S. defectivus y S. adjacens. En 1995, Kawamura et al2, basandose en secuencias del 16S rRNA de los NVS, demostraron que pertenecian a otro grupo diferente y propusieron el nuevo genero Abiotrophia, con dos especies, A. adiacens y A. defectiva. Posteriormente, se describieron dos especies mas, A. elegans3 y A. balaenopterae sp. nov4. Actualmente se ha reclasificado como genero Granulicatella con las especies: G. adiacens, G. elegans y G. balaenopterae5. Su morfologia microscopica depende del medio de cultivo, adoptando formas variables, desde cocos grampositivos, hasta cocobacilos pleomorficos gramvariable cuando no crecen en medios de cultivo Caso clinico