1. Activity Patterns in the Neuropil of Striatal Cholinergic Interneurons in Freely Moving Mice Represent Their Collective Spiking Dynamics
- Author
-
Wei-Hua Chiu, Gilad A. Jacobson, Gabriela J. Martins, Yara Atamna, Lior Tiroshi, Joshua A. Goldberg, Rotem Rehani, and Jose de Jesus Aceves Buendia
- Subjects
Male ,Dorsum ,Neuropil ,Somatic cell ,Action Potentials ,gradient reflective index (GRIN) lens ,Mice, Transgenic ,Striatum ,Motor Activity ,Tissue Culture Techniques ,03 medical and health sciences ,Spatio-Temporal Analysis ,0302 clinical medicine ,Interneurons ,Dopamine ,medicine ,Animals ,Calcium Signaling ,channelrhodopsin-2 ,Multiphoton imaging ,030304 developmental biology ,0303 health sciences ,Microscopy, Confocal ,Chemistry ,local field potentials (LFPs) ,General Neuroscience ,Dynamics (mechanics) ,General Medicine ,New Research ,Corpus Striatum ,pacemaker ,medicine.anatomical_structure ,two-photon laser scanning microscopy ,nervous system ,spatiotemporal patterns ,8.1 ,Sensory and Motor Systems ,Cholinergic ,Calcium ,Female ,Neuroscience ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Visual Abstract, Cholinergic interneurons (CINs) are believed to form synchronous cell assemblies that modulate the striatal microcircuitry and possibly orchestrate local dopamine release. We expressed GCaMP6s, a genetically encoded calcium indicator (GECIs), selectively in CINs, and used microendoscopes to visualize the putative CIN assemblies in the dorsal striatum of freely moving mice. The GECI fluorescence signal from the dorsal striatum was composed of signals from individual CIN somata that were engulfed by a widespread fluorescent neuropil. Bouts of synchronous activation of the cholinergic neuropil revealed patterns of activity that preceded the signal from individual somata. To investigate the nature of the neuropil signal and why it precedes the somatic signal, we target-patched GECI-expressing CINs in acute striatal slices in conjunction with multiphoton imaging or wide-field imaging that emulates the microendoscopes’ specifications. The ability to detect fluorescent transients associated with individual action potential was constrained by the long decay constant of GECIs (relative to common inorganic dyes) to slowly firing (
- Full Text
- View/download PDF