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Comfort temperature is important to investigate because the chosen office indoor temperatures affect the energy used in a building, and a thermally comfortable environment makes the occupants be more productive. The effects of temperature on comfort are broadly recognized for thermal comfort. Japanese office buildings are well equipped with air-conditioning systems to improve the thermal comfort of the occupants. The main objectives of this research were to compare the winter comfort temperature in mixed mode (MM) and heating, ventilation and air-conditioning (HVAC) office buildings and to investigate the relationship between the comfort temperature and the indoor air temperature. This study measured the thermal environmental conditions of the office buildings and surveyed the thermal comfort of the occupants. The field survey was conducted during winter in seven office buildings located in the Aichi prefecture of Japan. In total, 4466 subjective votes were collected from 46 occupants. The result suggested that the occupants were found to be more satisfied with the thermal environment of MM buildings than that of HVAC office buildings. Overall, 95% of comfort temperatures were in the range 22~28 °C in MM and HVAC buildings, which were higher than the indoor temperature of 20 °C recommended by the Japanese government. The comfort temperature was highly correlated to the indoor air temperature of the MM buildings than to that of HVAC buildings. This indicated that the occupants were more adapted towards the given thermal environment of MM buildings. [ABSTRACT FROM AUTHOR]