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THE ORIGINAL RESEARCH of Britton and Silvette in 1932 definitely established the relationship of the adrenal to carbohydrate metabolism. These authors showed that extracts of this gland were capable not only of restoring the blood sugar and liver and muscle glycogen stores in adrenalectomized animals but also would influence carbohydrate metabolism in the normal animal. At that time they suggested that the increase in hepatic glycogen might form the basis of a test for measuring the potency of adrenal extracts. It was not, however, until 1942, that Reinecke and Kendall published a quantitative method for the assay of adrenal hormones based upon the ability of these substances to cause deposition of glycogen in the livers of adrenalectomized fasted rats. More recently, in 1944, Olsen and his associates have evaluated the Reinecke Kendall test and have suggested certain modifications such as the pre-feeding of a high protein diet to increase the total response of the animal to a given dose of adrenal cortic...