1. H-score of 11β-hydroxylase and aldosterone synthase in the histopathological diagnosis of adrenocortical tumors
- Author
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Shumin Yang, Yunfeng He, Youde Cao, Ying Song, Bin Peng, Ming Xiao, Yao Zhang, Wenwen He, Qifu Li, Yi Yang, Linqiang Ma, Qingfeng Cheng, Jinbo Hu, Jun Yang, Yi Tang, and Ting Luo
- Subjects
Adenoma ,Adult ,Male ,Aldosterone synthase ,Receptors, Steroid ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Internal medicine ,Diabetes mellitus ,Adrenocortical Carcinoma ,medicine ,Cytochrome P-450 CYP11B2 ,Humans ,Adrenocortical carcinoma ,Steroid 11-beta-hydroxylase ,Aldosterone ,Aged ,Retrospective Studies ,Cell Nucleus ,Receiver operating characteristic ,biology ,business.industry ,Reproducibility of Results ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Adrenal Cortex Neoplasms ,Staining ,030220 oncology & carcinogenesis ,biology.protein ,Steroid 11-beta-Hydroxylase ,Female ,business - Abstract
To assess the diagnostic performance of the H-score of 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) in the histopathological diagnosis of adrenocortical tumors (ACT). We retrospectively evaluated 199 cases of ACT, of which 85 were diagnosed as aldosterone-producing adenoma (APA), 66 as cortisol-producing adenoma (CPA), 9 as aldosterone–cortisol co-secreting adenoma, 30 as nonhyperfunctioning adenoma, and 9 as adrenocortical carcinoma (ACC). Immunohistochemical staining was performed using anti-CYP11B1 and anti-CYP11B2 monoclonal antibodies. The staining was quantified by the McCarty’s H-score system. The diagnostic performance was assessed by the receiver operating characteristic curve (ROC). The H-score of CYP11B1 is highest in the CPA group and lowest in the ACC group. The H-score of CYP11B2 in the APA group is significantly higher than other ACT groups. The area under ROC (AUC) of an increased H-score of CYP11B2 (>65) for the diagnosis of APA was 0.971 (95%CI 0.937–0.990). The AUC of an increased H-score of CYP11B1 (>204) for the diagnosis of CPA was 0.725 (95%CI 0.658–0.786). The AUC of a decreased H-score of CYP11B1 (
- Published
- 2019
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