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4 results on '"Philip V'kovski"'

1. Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2

Author

Mitra Gultom, Matthias Licheri, Laura Laloli, Manon Wider, Marina Strässle, Philip V’kovski, Silvio Steiner, Annika Kratzel, Tran Thi Nhu Thao, Lukas Probst, Hanspeter Stalder, Jasmine Portmann, Melle Holwerda, Nadine Ebert, Nadine Stokar-Regenscheit, Corinne Gurtner, Patrik Zanolari, Horst Posthaus, Simone Schuller, Amanda Vicente-Santos, Andres Moreira-Soto, Eugenia Corrales-Aguilar, Nicolas Ruggli, Gergely Tekes, Veronika von Messling, Bevan Sawatsky, Volker Thiel, and Ronald Dijkman

Subjects
animal experimentation ethics, coronavirus disease, disease reservoirs, epidemics, epithelial cells, outbreaks, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally, and the number of worldwide cases continues to rise. The zoonotic origins of SARS-CoV-2 and its intermediate and potential spillback host reservoirs, besides humans, remain largely unknown. Because of ethical and experimental constraints and more important, to reduce and refine animal experimentation, we used our repository of well-differentiated airway epithelial cell (AEC) cultures from various domesticated and wildlife animal species to assess their susceptibility to SARS-CoV-2. We observed that SARS-CoV-2 replicated efficiently only in monkey and cat AEC culture models. Whole-genome sequencing of progeny viruses revealed no obvious signs of nucleotide transitions required for SARS-CoV-2 to productively infect monkey and cat AEC cultures. Our findings, together with previous reports of human-to-animal spillover events, warrant close surveillance to determine the potential role of cats, monkeys, and closely related species as spillback reservoirs for SARS-CoV-2.

Published
2021
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2. Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-Recommended Hand Rub Formulations and Alcohols

Author

Annika Kratzel, Daniel Todt, Philip V’kovski, Silvio Steiner, Mitra Gultom, Tran Thi Nhu Thao, Nadine Ebert, Melle Holwerda, Jörg Steinmann, Daniela Niemeyer, Ronald Dijkman, Günter Kampf, Christian Drosten, Eike Steinmann, Volker Thiel, and Stephanie Pfaender

Subjects
ethanol, 2-propanol, hand sanitizer, World Health Organization, coronavirus disease, respiratory infections, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Infection control instructions call for use of alcohol-based hand rub solutions to inactivate severe acute respiratory syndrome coronavirus 2. We determined the virucidal activity of World Health Organization–recommended hand rub formulations, at full strength and multiple dilutions, and of the active ingredients. All disinfectants demonstrated efficient virus inactivation.

Published
2020
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3. Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2

Author

Philip V'kovski, Tran Thi Nhu Thao, Andres Moreira-Soto, Bevan Sawatsky, Nicolas Ruggli, Patrik Zanolari, Simone Schuller, Manon Wider, Volker Thiel, Eugenia Corrales-Aguilar, Annika Kratzel, Horst Posthaus, Melle Holwerda, Corinne Gurtner, Nadine Stokar-Regenscheit, Amanda Vicente-Santos, Lukas Probst, Hanspeter Stalder, Nadine Ebert, Gergely Tekes, Matthias Licheri, Veronika von Messling, Laura Laloli, Jasmine Portmann, Ronald Dijkman, Marina Strässle, Silvio Steiner, and Mitra L. Gultom

Subjects
Microbiology (medical), Disease reservoir, Epidemiology, Susceptibility of Well-Differentiated Airway Epithelial Cell Cultures from Domestic and Wild Animals to Severe Acute Respiratory Syndrome Coronavirus 2, viruses, Respiratory System, severe acute respiratory syndrome 2, Animals, Wild, Infectious and parasitic diseases, RC109-216, Biology, epidemics, 03 medical and health sciences, respiratory infections, medicine, Animals, Humans, Respiratory system, Animal testing, skin and connective tissue diseases, 030304 developmental biology, Original Research, Whole genome sequencing, 0303 health sciences, whole genome sequencing, CATS, 030306 microbiology, SARS-CoV-2, Research, fungi, Outbreak, COVID-19, disease reservoirs, respiratory system, Virology, Epithelium, epithelial cells, 3. Good health, zoonoses, respiratory tract diseases, Infectious Diseases, medicine.anatomical_structure, coronavirus disease, outbreaks, animal experimentation ethics, Medicine, Airway
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally, and the number of worldwide cases continues to rise. The zoonotic origins of SARS-CoV-2 and its intermediate and potential spillback host reservoirs, besides humans, remain largely unknown. Because of ethical and experimental constraints and more important, to reduce and refine animal experimentation, we used our repository of well-differentiated airway epithelial cell (AEC) cultures from various domesticated and wildlife animal species to assess their susceptibility to SARS-CoV-2. We observed that SARS-CoV-2 replicated efficiently only in monkey and cat AEC culture models. Whole-genome sequencing of progeny viruses revealed no obvious signs of nucleotide transitions required for SARS-CoV-2 to productively infect monkey and cat AEC cultures. Our findings, together with previous reports of human-to-animal spillover events, warrant close surveillance to determine the potential role of cats, monkeys, and closely related species as spillback reservoirs for SARS-CoV-2.

Published
2021

4. Inactivation of Severe Acute Respiratory Syndrome Coronavirus 2 by WHO-Recommended Hand Rub Formulations and Alcohols

Author

Jörg Steinmann, Annika Kratzel, Silvio Steiner, Stephanie Pfaender, Ronald Dijkman, Eike Steinmann, Günter Kampf, Volker Thiel, Daniela Niemeyer, Melle Holwerda, Philip V'kovski, Tran Thi Nhu Thao, Christian Drosten, Nadine Ebert, Daniel Todt, and Mitra Gultom

Subjects
Epidemiology, Expedited, coronavirus, Medizin, lcsh:Medicine, Inactivation of SARS-CoV-2 by WHO-Recommended Hand Rub Formulations and Alcohols, medicine.disease_cause, 0302 clinical medicine, Infection control, 030212 general & internal medicine, Coronavirus, integumentary system, Dispatch, hand sanitizer, 3. Good health, Infectious Diseases, coronavirus disease, Coronavirus Infections, severe acute respiratory syndrome coronavirus 2, Hand Disinfection, Microbiology (medical), 2019-20 coronavirus outbreak, Virus inactivation, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Pneumonia, Viral, 030231 tropical medicine, World Health Organization, World health, lcsh:Infectious and parasitic diseases, 2019 novel coronavirus disease, Microbiology, respiratory infections, Betacoronavirus, 03 medical and health sciences, Hand sanitizer, 2-propanol, medicine, Humans, lcsh:RC109-216, viruses, Pandemics, SARS, Hand rub, SARS-CoV-2, lcsh:R, COVID-19, zoonoses, Alcohols, Virus Inactivation, ethanol, Disinfectants
Abstract

Infection control instructions call for use of alcohol-based hand rub solutions to inactivate severe acute respiratory syndrome coronavirus 2. We determined the virucidal activity of World Health Organization-recommended hand rub formulations, at full strength and multiple diluÂtions, and of the active ingredients. All disinfectants demAônstrated efficient virus inactivation. CA extern

Published
2020
Full Text
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