10 results on '"Norio Ohashi"'
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2. Spotted Fever Group Rickettsiae in Inner Mongolia, China, 2015–2016
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Gaowa, Wulantuya, Xuhong Yin, Shengchun Guo, Chunlian Ding, Minzhi Cao, Hiroki Kawabata, Kozue Sato, Shuji Ando, Hiromi Fujita, Fumihiko Kawamori, Hongru Su, Masahiko Shimada, Yuko Shimamura, Shuichi Masuda, and Norio Ohashi
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Rickettsia ,Rickettsia raoultii ,Rickettsia aeschlimannii ,Hyalomma asiaticum ,Dermacentor nuttalli ,Hyalomma marginatum ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We found Rickettsia raoultii infection in 6/261 brucellosis-negative patients with fever of unknown origin in brucellosis-endemic Inner Mongolia, China. We further identified Hyalomma asiaticum ticks associated with R. raoultii, H. marginatum ticks associated with R. aeschlimannii, and Dermacentor nuttalli ticks associated with both rickettsiae species in the autonomous region.
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- 2018
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3. Human Granulocytic Anaplasmosis, Japan
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Norio Ohashi, Gaowa, Wuritu, Fumihiko Kawamori, Dongxing Wu, Yuko Yoshikawa, Seizou Chiya, Kazutoshi Fukunaga, Toyohiko Funato, Masaaki Shiojiri, Hideki Nakajima, Yoshiji Hamauzu, Ai Takano, Hiroki Kawabata, Shuji Ando, and Toshio Kishimoto
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Anaplasma phagocytophilum ,anaplasmosis ,Rickettsia japonica ,rickettsiosis ,p44/msp2 ,16S rDNA ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We retrospectively confirmed 2 cases of human Anaplasma phagocytophilum infection. Patient blood samples contained unique p44/msp2 for the pathogen, and antibodies bound to A. phagocytophilum antigens propagated in THP-1 rather than HL60 cells. Unless both cell lines are used for serodiagnosis of rickettsiosis-like infections, cases of human granulocytic anaplasmosis could go undetected.
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- 2013
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4. Anaplasma phagocytophilum–infected Ticks, Japan
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Norio Ohashi, Megumi Inayoshi, Kayoko Kitamura, Fumihiko Kawamori, Daizoh Kawaguchi, Yuusaku Nishimura, Hirotaka Naitou, Midori Hiroi, and Toshiyuki Masuzawa
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Anaplasma phagocytophilum ,anaplasmosis ,Ixodes ,Japan ,p44 protein ,16S ribosomal RNA ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We report Anaplasma phagocytophilum infection of Ixodes persulcatus and I. ovatus ticks in Japan. Unique p44/msp2 paralogs (and/or 16S rRNA genes) were detected in tick tissues, salivary glands, and spleens of experimentally infected mice. These findings indicate the public health threat of anaplasmosis in Japan.
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- 2005
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5. Rickettsiae in Ticks, Japan, 2007–2011
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Gaowa, Norio Ohashi, Minami Aochi, Wuritu, Dongxing Wu, Yuko Yoshikawa, Fumihiko Kawamori, Toshiro Honda, Hiromi Fujita, Nobuhiro Takada, Yosaburo Oikawa, Hiroki Kawabata, Shuji Ando, and Toshio Kishimoto
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Rickettsiales ,Rickettsia ,rickettsiae ,Anaplasma phagocytophilum ,Ehrlichia ,tick-borne infections ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2013
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6. Anaplasma phagocytophilum Antibodies in Humans, Japan, 2010–2011
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Yuko Yoshikawa, Norio Ohashi, Dongxing Wu, Fumihiko Kawamori, Asaka Ikegaya, Takuya Watanabe, Kazuhito Saitoh, Daisuke Takechi, Yoichi Murakami, Daisuke Shichi, Katsumi Aso, and Shuji Ando
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Anaplasma phagocytophilum ,anaplasmosis ,serodiagnosis ,p44/msp2 ,P44 immunodominant protein ,Japan ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2014
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7. Anaplasma phagocytophilum–infected Ticks, Japan
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Kayoko Kitamura, Yuusaku Nishimura, Fumihiko Kawamori, Daizoh Kawaguchi, Norio Ohashi, Midori Hiroi, Megumi Inayoshi, Hirotaka Naitou, and Toshiyuki Masuzawa
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DNA, Bacterial ,Male ,Microbiology (medical) ,Ehrlichiosis ,Epidemiology ,animal diseases ,Molecular Sequence Data ,lcsh:Medicine ,Biology ,Ixodes persulcatus ,Tick ,Salivary Glands ,lcsh:Infectious and parasitic diseases ,Microbiology ,Immunocompromised Host ,Mice ,Japan ,parasitic diseases ,medicine ,Animals ,lcsh:RC109-216 ,anaplasmosis ,Cyclophosphamide ,Phylogeny ,Ixodes ,lcsh:R ,fungi ,Dispatch ,Sequence Analysis, DNA ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,16S ribosomal RNA ,Virology ,Anaplasma phagocytophilum ,Infectious Diseases ,Bacterial Outer Membrane Proteins ,Female ,p44 protein ,Anaplasmosis - Abstract
We report Anaplasma phagocytophilum infection of Ixodes persulcatus and I. ovatus ticks in Japan. Unique p44/msp2 paralogs (and/or 16S rRNA genes) were detected in tick tissues, salivary glands, and spleens of experimentally infected mice. These findings indicate the public health threat of anaplasmosis in Japan.
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- 2005
8. Rickettsiae in Ticks, Japan, 2007–2011
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Dongxing Wu, Yuko Yoshikawa, Gaowa, Shuji Ando, Hiromi Fujita, Toshiro Honda, Wuritu, Nobuhiro Takada, Minami Aochi, Toshio Kishimoto, Fumihiko Kawamori, Norio Ohashi, Hiroki Kawabata, and Yosaburo Oikawa
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Letter ,tickborne infections ,Rickettsiales ,lcsh:Medicine ,Genes, Insect ,p28/omp-1 ,Polymerase Chain Reaction ,Salivary Glands ,p44/msp2 ,Japan ,RNA, Ribosomal, 16S ,rickettsiae ,Ehrlichia chaffeensis ,Rickettsia ,bacteria ,Genetics ,biology ,Phylogenetic tree ,Ehrlichia ,RNA, Bacterial ,Infectious Diseases ,Epidemiological Monitoring ,surveillance ,epidemiology ,ompA ,Bacterial Outer Membrane Proteins ,Anaplasma phagocytophilum ,Microbiology (medical) ,Nymph ,Risk ,Tick ,lcsh:Infectious and parasitic diseases ,16S rDNA ,Animals ,lcsh:RC109-216 ,Letters to the Editor ,Rickettsia japonica ,spotted fever group ,Ixodes ,Japanese spotted fever ,lcsh:R ,DNA ,Haemaphysalis ,biology.organism_classification ,Virology ,Spotted fever ,Insect Vectors ,tick-borne infections ,Candidatus ,gltA ,Multilocus Sequence Typing - Abstract
To the Editor: Japanese spotted fever (JSF), caused by Rickettsia japonica, is the most prevalent tickborne infectious disease in Japan (1), occurring most frequently in central and western regions (http://idsc.nih.go.jp/idwr/CDROM/Main.html [in Japanese]). Cases of unknown fever with rickettsiosis-like symptoms not associated with JSF have been reported in JSF-endemic regions of Japan (2). Several spotted fever group (SFG) rickettsiae (R. japonica, R. heilongjiangensis, R. helvetica, R. tamurae, R. asiatica, Candidatus R. tarasevichiae) and other related Rickettsia spp. have been identified in Japan (1,3–6). Human infections with R. heilongjiangensis and R. tamurae have been confirmed (3,5), and Anaplasma phagocytophilum and Ehrlichia chaffeensis, known human pathogens, have been detected in ticks and deer in Japan. We conducted this study to determine the risk in central and western Japan for human exposure to ticks harboring SFG rickettsiae, A. phagocytophilum, or Ehrlichia spp. In 2007–2011, we collected 827 Haemaphysalis, Amblyomma, and Ixodes spp. ticks (392 adults, 435 nymphs) by flagging vegetation in the prefectures of Shizuoka, Mie, Wakayama, Kagoshima, Nagasaki (Goto Island), and Okinawa (the main island and Yonaguni Island) (Technical Appendix Figure 1). We extracted DNA from the salivary glands of each tick and performed PCR to amplify gltA, 16S rDNA, and ompA of SFG rickettsiae. To detect A. phagocytophilum and Ehrlichia spp., we performed nested PCR targeting the p44/msp2 and p28/omp-1 multigenes, respectively. PCR gltA screening revealed SFG rickettsiae in 181 (21.9%) of the 827 ticks (Table). We obtained nearly full-length (1.1-kb) gltA sequences and classified them into 5 groups by phylogenetic analyses (Technical Appendix Figure 2). Sequences for groups 1 (prevalence 1.0%) and 2 (prevalence 3.2%) were identified as R. japonica YH (GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AP011533","term_id":"348592266","term_text":"AP011533"}}AP011533) and R. tamurae (GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AF394896","term_id":"21360604","term_text":"AF394896"}}AF394896), respectively (Table). Group 3 (prevalence 15.1%) sequences were identical to that of Rickettsia sp. LON (GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AB516964","term_id":"256353425","term_text":"AB516964"}}AB516964). The sequence for group 4 (prevalence 1.6%) was closely related to that for R. raoultii strain Khabarovsk (98.8% similarity), and a part of the sequence (342 bp) was identical to that of Rickettsia sp. Hf 151 (GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"AB114815","term_id":"33146352","term_text":"AB114815"}}AB114815). Group 5 consisted of 4 newly identified rickettsiae (Technical Appendix Figure 2). Of these 4 rickettsiae, 3 (Mie311, Goto13, and Mie334) were closely related to R. raoultii strain Khabarovsk (98.0% identity) and 1 (Mie201) was similar to Candidatus R. principis (99.7% identity). Table PCR survey results for Haemaphysalis, Amblyomma, and Ixodes spp. ticks tested for rickettsiae, central and western Japan, 2007–2011* We further analyzed the 16S rDNA and ompA in gltA-positive tick samples. The 16S rDNA and ompA for group 1 samples shared 100% identity with 16S rDNA and ompA of R. japonica YH ({"type":"entrez-nucleotide","attrs":{"text":"AP011533","term_id":"348592266","term_text":"AP011533"}}AP011533). The 16S rDNA of group 2 was identical to that of R. tamurae ({"type":"entrez-nucleotide","attrs":{"text":"AY049981","term_id":"21360334","term_text":"AY049981"}}AY049981). In groups 3–5, some of the specific amplicons in 16S rDNA or ompA could be detected; their sequences were confirmed to be similar (but not identical) to those of several known rickettsial sequences. We amplified the p44/msp2 amplicons of A. phagocytophilum from 25 (3%) of 827 ticks (Table). By cloning (TA Cloning Kit; Life Technologies, Carlsbad, CA, USA) and sequencing these amplicons, we obtained and identified 60 new TA-clone sequences (366–507 bp) for p44/msp2 (GenBank accession nos. {"type":"entrez-nucleotide-range","attrs":{"text":"JQ697880-JQ697950","start_term":"JQ697880","end_term":"JQ697950","start_term_id":"383088493","end_term_id":"383088630"}}JQ697880-JQ697950); these sequences may include a potentially novel Anaplasma species. (7). Ehrlichia p28/omp-1 was detected from 2 (0.2%) of the 827 ticks. Of 5 TA-clone sequences (284–315 bp) obtained from the 2 ticks, 2 from an A. testudinarium tick (GenBank accession nos. {"type":"entrez-nucleotide","attrs":{"text":"JQ697886","term_id":"383088503","term_text":"JQ697886"}}JQ697886 and {"type":"entrez-nucleotide","attrs":{"text":"JQ697887","term_id":"383088505","term_text":"JQ697887"}}JQ697887) shared 83.3%–86.7% similarity with E. ruminantium Gardel Map-1 (GenBank accession no. YP196842), and 3 from an H. longicornis tick (GenBank accession nos. {"type":"entrez-nucleotide-range","attrs":{"text":"JQ697888-JQ697890","start_term":"JQ697888","end_term":"JQ697890","start_term_id":"383088507","end_term_id":"383088511"}}JQ697888-JQ697890) showed the closest relationship to E. ewingii omp-1–15 (67%–73% similarity; GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"EF116932","term_id":"133711094","term_text":"EF116932"}}EF116932). We identified the tick species associated with R. japonica as H. formosensis, H. hystricis, and H. cornigera, and another study reported an association with Dermacentor taiwanensis, H. flava, H. longicornis, and I. ovatus (4). In our study and previous studies, the tick species associated with A. phagocytophilum in Japan were identified as H. formosensis, H. longicornis, H. megaspinosa, A. testudinarium, I. ovatus, and I. persulcatus (8). Thus, it appears that 3 tick species (H. formosensis, H. longicornis, and I. ovatus) are associated with R. japonica and A. phagocytophilum. In addition, in an H. formosensis tick, we detected an SFG rickettsia that is closely related to R. raoultii, the etiologic agent of Dermacentor-borne necrosis erythema and lymphadenopathy in Europe and Russia (9). We detected Candidatus R. principis in H. flava in Japan; this species was previously detected in H. japonica douglasi and H. danieli ticks in Russia and China, respectively, (10). And, we found a high prevalence of R. tamurae in A. testidinarium ticks; Imaoka et al. (5) recently reported that R. tamurae causes local skin inflammation without general JSP-like symptoms. We did not detect the human pathogen E. chaffeensis, but we identified 2 potentially new Ehrlichia species. Our findings contribute to the known risks for exposure to Rickettsia-related pathogens in central and western Japan. Further studies may be required for the surveillance of additional pathogens, such as Candidatus Neoehrlichia mikurensis (2), which was recently recognized as a human pathogen. Technical Appendix: Phylogenetic classification of Rickettsia spp. gltA sequences detected in ticks during 2007–2011 in central and western Japan and locations of tick collection sites. Click here to view.(154K, pdf)
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- 2013
9. Anaplasma phagocytophilum Antibodies in Humans, Japan, 2010–2011
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Dongxing Wu, Asaka Ikegaya, Yuko Yoshikawa, Daisuke Takechi, Katsumi Aso, Kazuhito Saitoh, Fumihiko Kawamori, Shuji Ando, Norio Ohashi, Takuya Watanabe, Daisuke Shichi, Gaowa, and Yoichi Murakami
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Microbiology (medical) ,Letter ,Human granulocytic anaplasmosis ,P44 immunodominant protein ,Epidemiology ,animal diseases ,vector-borne infections ,lcsh:Medicine ,Biology ,p44/msp2 ,lcsh:Infectious and parasitic diseases ,Serology ,Antigen ,Japan ,parasitic diseases ,medicine ,lcsh:RC109-216 ,anaplasmosis ,Letters to the Editor ,bacteria ,serodiagnosis ,lcsh:R ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Virology ,Anaplasma phagocytophilum ,Infectious Diseases ,Cell culture ,biology.protein ,Anaplasmosis ,Antibody ,Bacterial outer membrane - Abstract
To the Editor: Human granulocytic anaplasmosis (HGA) is an emerging tick-borne infectious disease caused by Anaplasma phagocytophilum, an obligatory intracellular bacterium (1). Recently, 2 cases of HGA were identified by a retrospective study in Japan (2). For serodiagnosis of HGA, A. phagocytophilum propagated in HL60 cells is usually used as an antigen, especially by indirect immunofluorescent assay (IFA) (3). However, the serum from these 2 patients in Japan reacted with antigens of A. phagocytophilum cultured in THP-1 cells rather than in HL60 cells in IFA (2). In A. phagocytophilum, a p44/msp2 multigene family encoding multiple 44-kDa immunodominant major outer membrane protein species (so-called P44) exists on the genome, and these multigenes are similar, but not identical, to each other, and the bacterium generates antigenic variations because of gene conversion (4). The previous studies showed that A. phagocytophilum expresses predominantly 2 species of p44/msp2 transcripts in THP-1 cells, but it produces the variation of P44 protein species in HL60 cells (2,5). This finding strongly suggested that A. phagocytophilum grown in THP-1 cells differs serologically from that in HL60 cells. Our serologic analysis found 4 recent cases of HGA in Japan by using infected THP-1 and HL60 cells as antigens, and some P44 immunoreactive protein species of A. phagocytophilum that were associated with the respective cell line cultures, binding to antibodies from the 4 patients’ serum, also were identified.
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- 2014
10. Anaplasma phagocytophilum Antibodies in Humans, Japan, 2010-2011.
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Gaowa, Yuko Yoshikawa, Norio Ohashi, Dongxing Wu, Fumihiko Kawamori, Asaka Ikegaya, Takuya Watanabe, Kazuhito Saitoh, Daisuke Takechi, Yoichi Murakami, Daisuke Shichi, Katsumi Aso, and Ando, Shuji
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ANAPLASMOSIS ,TICK-borne diseases ,ANAPLASMA phagocytophilum - Abstract
A letter to the editor about cases of human granulocytic anaplasmosis (HGA), a tick-borne infectious disease caused by Anaplasma phagocytophilum, is presented.
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- 2014
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