1. Detection of blaIMP4 and blaNDM1 harboring Klebsiella pneumoniae isolates in a university hospital in Malaysia
- Author
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Rosliza Abdul Rahman, Chan Yean Yean, Nurul Izzati Hamzan, Zaidah Abdul Rahman, and Habsah Hasan
- Subjects
Epidemiology ,Klebsiella pneumoniae ,medicine.drug_class ,Health, Toxicology and Mutagenesis ,030231 tropical medicine ,Antibiotics ,Gene Expression ,Microbial Sensitivity Tests ,Carbapenem-resistant enterobacteriaceae ,beta-Lactamases ,Microbiology ,Hospitals, University ,carbapenemase ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,Bacterial Proteins ,Drug Resistance, Multiple, Bacterial ,Drug Resistance, Bacterial ,medicine ,Humans ,Modified Hodge test ,Infection control ,Original Research Article ,030212 general & internal medicine ,Etest ,biology ,Health Policy ,Malaysia ,Public Health, Environmental and Occupational Health ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,Virology ,Enterobacteriaceae ,Anti-Bacterial Agents ,Klebsiella Infections ,Molecular Typing ,Cross-Sectional Studies ,carbapenem-resistant Enterobacteriaceae ,Carbapenems ,Colistin ,medicine.drug - Abstract
Background : Antibiotic resistance among Enterobacteriaceae posts a great challenge to the health care service. The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) is attracting significant attention due to its rapid and global dissemination. The infection is associated with significant morbidity and mortality, thus creating challenges for infection control and managing teams to curb the infection. In Southeast Asia, there have been limited reports and subsequent research regarding CRKP infections. Thus, the study was conducted to characterize CRKP that has been isolated in our setting. Methods : A total of 321 K. pneumoniae were included in the study. Each isolate went through an identification process using an automated identification system. Phenotypic characterization was determined using disk diffusion, modified Hodge test, Epsilometer test, and inhibitor combined disk test. Further detection of carbapenemase genes was carried out using polymerase chain reaction and confirmed by gene sequence analysis. Results : All together, 13 isolates (4.05%) were CRKP and the majority of them were resistant to tested antibiotics except colistin and tigercycline. Among seven different carbapenemase genes studied ( bla KPC , bla IMP , bla SME , bla NDM , bla IMI, bla VIM , and bla OXA ), only two, bla IMP4 (1.87%) and bla NDM1 (2.18%), were detected in our setting. Conclusion : Evidence suggests that the prevalence of CRKP in our setting is low, and knowledge of Carbapenem-resistant Enterobacteriaceae and CRKP has improved and become available among clinicians. Keywords: carbapenem-resistant Enterobacteriaceae; carbapenemase ; Klebsiella pneumoniae; Modified Hodge test (Published: 10 March 2015) Citation: Emerg Health Threats J 2015, 8 : 26011 - http://dx.doi.org/10.3402/ehtj.v8.26011
- Published
- 2015