1. Pds5 promotes cohesin acetylation and stable cohesin-chromosome interaction
- Author
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Vaur, Sabine, Feytout, Amélie, Vazquez, Stéphanie, Javerzat, Jean-Paul, Javerzat, Jean‐Paul, Institut de biochimie et génétique cellulaires (IBGC), and Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)
- Subjects
G2 Phase ,MESH: Mutation ,Chromosomal Proteins, Non-Histone ,[SDV]Life Sciences [q-bio] ,Mitosis ,MESH: Carrier Proteins ,Cell Cycle Proteins ,Chromatids ,Biochemistry ,S Phase ,03 medical and health sciences ,0302 clinical medicine ,MESH: Cell Cycle Proteins ,MESH: Chromosomal Proteins, Non-Histone ,Schizosaccharomyces ,Genetics ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,biology ,Cohesin ,Scientific Reports ,MESH: S Phase ,Chromosome ,MESH: Chromatids ,MESH: Schizosaccharomyces pombe Proteins ,Acetylation ,MESH: Mitosis ,biology.organism_classification ,Cell biology ,Schizosaccharomyces pombe Proteins ,MESH: G2 Phase ,MESH: Schizosaccharomyces ,Carrier protein ,Mutation ,Chromatid ,biological phenomena, cell phenomena, and immunity ,Carrier Proteins ,030217 neurology & neurosurgery ,MESH: Acetylation - Abstract
International audience; Pds5 and Wpl1 act as anti-establishment factors preventing sister-chromatid cohesion until counteracted in S-phase by the cohesin acetyl-transferase Eso1. However, Pds5 is also required to maintain sister-chromatid cohesion in G2. Here, we show that Pds5 is essential for cohesin acetylation by Eso1 and ensures the maintenance of cohesion by promoting a stable cohesin interaction with replicated chromosomes. The latter requires Eso1 only in the presence of Wapl, indicating that cohesin stabilization relies on Eso1 only to neutralize the anti-establishment activity. We suggest that Eso1 requires Pds5 to counteract anti-establishment. This allows both cohesion establishment and Pds5-dependent stable cohesin binding to chromosomes.
- Published
- 2012