1. tRNA prefers to kiss.
- Author
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Scarabino D, Crisari A, Lorenzini S, Williams K, and Tocchini-Valentini GP
- Subjects
- Anticodon, Base Sequence, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Complementary, RNA, Fungal chemistry, RNA, Fungal metabolism, RNA, Transfer, Phe chemistry, RNA, Transfer, Phe metabolism, Saccharomyces cerevisiae genetics
- Abstract
Six RNA aptamers that bind to yeast phenylalanine tRNA were identified by in vitro selection from a random-sequence pool. The two most abundantly represented aptamers interact with the tRNA anticodon loop, each through a sequence block with perfect Watson-Crick complementarity to the loop. It was possible to truncate one of these aptamers to a simple hairpin loop that forms a classical 'kissing complex' with the anticodon loop. Three other aptamers have nearly complete complementarity to the anticodon loop. The sixth aptamer has two sequence blocks, one complementary to the tRNA T loop and the other to the D loop; this aptamer binds better to a mutant tRNA that disrupts the normal D-loop/T-loop tertiary interaction than to the wild-type tRNA. Selection of complements to tRNA loops occurred despite an attempt to direct binding to tertiary structural features of tRNA. This serves as a reminder of how special the RNA-RNA interactions are that are not based on complementarity. Nonetheless, these aptamers must present the tRNA complement in some special structural context; the simple single-strand complement of the anticodon loop did not bind tRNA effectively.
- Published
- 1999
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